thiI:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

thiI

Gene Synonym(s)

ECK0417, b0423, JW0413, nuvC, thiJ, yajK, nuvA, yajJ[1], yajJ

Product Desc.

THII[2][3]

Thiamin biosynthesis, required for adenylation/thiocarboxylation of ThiS C-terminus in vivo; also required for 4-thiouridine modification of tRNA; sulfurtransferase[4]

Product Synonyms(s)

sulfurtransferase required for thiamine and 4-thiouridine biosynthesis[1], B0423[2][1], NuvA[2][1], YajK[2][1], NuvC[2][1], ThiI[2][1] , ECK0417, JW0413, nuvA, yajJ, yajK, b0423

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): thiI[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

thiI

Mnemonic

Thiamin (and thiazole)

Synonyms

ECK0417, b0423, JW0413, nuvC, thiJ, yajK, nuvA, yajJ[1], yajJ

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

9.5 minutes 

MG1655: 440773..442221
<gbrowseImage> name=NC_000913:440773..442221 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 409971..411419
<gbrowseImage> name=NC_012967:409971..411419 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 343532..344980
<gbrowseImage> name=NC_012759:343532..344980 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 440773..442221
<gbrowseImage> name=NC_007779:440773..442221 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 380104..381552
<gbrowseImage> name=NC_010473:380104..381552 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

thiI(del) (Keio:JW0413)

deletion

deletion

PMID:16738554

Shigen

CGSC8580[5]

thiI780(del)::kan

PMID:16738554

CGSC:103140

lac valS(TS) relA+ nuvC

point mutation

UV resistance

Near-UV irradiation resistance, table 1.

PMID:6178725

Experimental strain: RYH101

nuvA -

Resistant to

Near UV-irradiation (3 to 400 nm)

PMID:357427

Also referred to as s4U -

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0413

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAGTTTATCATTAAATTGTT

Primer 2:CCCGGGCGATATACCTTCACATT

19F6

Kohara Phage

Genobase

PMID:3038334

2H5

Kohara Phage

Genobase

PMID:3038334

tsx-247::Tn10

Linked marker

CAG12148 = CGSC7337[5]

est. P1 cotransduction: 73% [6]

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[5]

est. P1 cotransduction: 51% [6]
Synonyms:zba-3054::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6238

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13273

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002849

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945067

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3058

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001470

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ThiI

Synonyms

sulfurtransferase required for thiamine and 4-thiouridine biosynthesis[1], B0423[2][1], NuvA[2][1], YajK[2][1], NuvC[2][1], ThiI[2][1] , ECK0417, JW0413, nuvA, yajJ, yajK, b0423

Product description

THII[2][3]

Thiamin biosynthesis, required for adenylation/thiocarboxylation of ThiS C-terminus in vivo; also required for 4-thiouridine modification of tRNA; sulfurtransferase[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003723

RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003720

F

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020536

F

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0694

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00021

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003720

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020536

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0009228

thiamin biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00021

P

Seeded from EcoCyc (v14.0)

complete

GO:0009228

thiamin biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003720

P

Seeded from EcoCyc (v14.0)

complete

GO:0009228

thiamin biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020536

P

Seeded from EcoCyc (v14.0)

complete

GO:0009228

thiamin biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0784

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rplB

PMID:16606699

Experiment(s):EBI-1136559

Protein

livF

PMID:16606699

Experiment(s):EBI-1136559

Protein

rplA

PMID:16606699

Experiment(s):EBI-1136559

Protein

rpsN

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKFIIKLFPE ITIKSQSVRL RFIKILTGNI RNVLKHYDET LAVVRHWDNI EVRAKDENQR
LAIRDALTRI PGIHHILEVE DVPFTDMHDI FEKALVQYRD QLEGKTFCVR VKRRGKHDFS
SIDVERYVGG GLNQHIESAR VKLTNPDVTV HLEVEDDRLL LIKGRYEGIG GFPIGTQEDV
LSLISGGFDS GVSSYMLMRR GCRVHYCFFN LGGAAHEIGV RQVAHYLWNR FGSSHRVRFV
AINFEPVVGE ILEKIDDGQM GVILKRMMVR AASKVAERYG VQALVTGEAL GQVSSQTLTN
LRLIDNVSDT LILRPLISYD KEHIINLARQ IGTEDFARTM PEYCGVISKS PTVKAVKSKI
EAEEEKFDFS ILDKVVEEAN NVDIREIAQQ TEQEVVEVET VNGFGPNDVI LDIRSIDEQE
DKPLKVEGID VVSLPFYKLS TKFGDLDQNK TWLLWCERGV MSRLQALYLR EQGFNNVKVY RP
Length

482

Mol. Wt

54.973 kDa

pI

6.6 (calculated)

Extinction coefficient

41,370 - 41,995 (calc based on 13 Y, 4 W, and 5 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

175..369

PF02568 Thiamine biosynthesis protein (ThiI)

PMID:19920124

Domain

75..164

PF02926 THUMP domain

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=thiI taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128408

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945067

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001470

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P77718

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6238

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13273

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945067

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002849

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3058

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

4.53E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

3830

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

483

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1255

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

thiI

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:440753..440793 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0423 (EcoliWiki Page)

NCBI GEO profiles for thiI

microarray

GenExpDB:b0423 (EcoliWiki Page)

Summary of data for thiI from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (440478..440812) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ10; Well:G6[7]

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Notes

Accessions Related to thiI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6238

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3058

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0423

EcoGene

EcoGene:EG13273

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002849

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001470

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

YAJK

From SHIGELLACYC

E. coli O157

YAJK

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:52402

Superfamily (EcoliWiki Page)

SUPERFAMILY:52821

Superfamily (EcoliWiki Page)

SUPERFAMILY:143437

Pfam (EcoliWiki Page)

PF02568 Thiamine biosynthesis protein (ThiI)

Pfam (EcoliWiki Page)

PF02926 THUMP domain

EcoCyc

EcoCyc:G6238

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13273

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002849

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3058

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001470

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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