tehB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

tehB

Gene Synonym(s)

ECK1423, b1430, JW1426[1], JW1426

Product Desc.

tellurite resistance protein[2][3]

Multidrug resistance; K+-tellurite, ethidium and proflavin transport; binds SAM; dimer[4]

Product Synonyms(s)

predicted S-adenosyl-L-methionine-dependent methyltransferase[1], B1430[2][1], TehB[2][1] , ECK1423, JW1426, b1430

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): tehAB[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tehB

Mnemonic

Tellurite resistance

Synonyms

ECK1423, b1430, JW1426[1], JW1426

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

32.32 minutes 

MG1655: 1499586..1500179
<gbrowseImage> name=NC_000913:1499586..1500179 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1471671..1472264
<gbrowseImage> name=NC_012967:1471671..1472264 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1391645..1392238
<gbrowseImage> name=NC_012759:1391645..1392238 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1503276..1503869
<gbrowseImage> name=NC_007779:1503276..1503869 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1590181..1590774
<gbrowseImage> name=NC_010473:1590181..1590774 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔtehB (Keio:JW1426)

deletion

deletion

PMID:16738554

Shigen
CGSC9248[5]

tehB::Tn5KAN-2 (FB20322)

Insertion at nt 163 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20322

contains pKD46

tehB::Tn5KAN-2 (FB20323)

Insertion at nt 163 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20323

does not contain pKD46

ΔtehB790::kan

PMID:16738554

CGSC:103124


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1426

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCATCATTCGTGACGAAAACTA

Primer 2:CCTTTTTTACGTGCCAGCATCGT

1A6

Kohara Phage

Genobase

PMID:3038334

10D6

Kohara Phage

Genobase

PMID:3038334

trg-2::Tn10

Linked marker

CAG12026 = CGSC7376[5]

est. P1 cotransduction: 83% [6]

zdd-235::Tn10

Linked marker

CAG18461 = CGSC7378[5]

est. P1 cotransduction: 23% [6]
Synonyms:zdc-235::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11884

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11884

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001814

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945979

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1830

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004772

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

TehB

Synonyms

predicted S-adenosyl-L-methionine-dependent methyltransferase[1], B1430[2][1], TehB[2][1] , ECK1423, JW1426, b1430

Product description

tellurite resistance protein[2][3]

Multidrug resistance; K+-tellurite, ethidium and proflavin transport; binds SAM; dimer[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004537

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0008757

S-adenosylmethionine-dependent methyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004537

F

Seeded from EcoCyc (v14.0)

complete

GO:0046677

response to antibiotic

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0046

P

Seeded from EcoCyc (v14.0)

complete

GO:0046690

response to tellurium ion

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004537

P

Seeded from EcoCyc (v14.0)

complete

GO:0046690

response to tellurium ion

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0778

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

yibL

PMID:16606699

Experiment(s):EBI-1139830

Protein

groL

PMID:16606699

Experiment(s):EBI-1139830

Protein

rplB

PMID:16606699

Experiment(s):EBI-1139830

Protein

mqo

PMID:16606699

Experiment(s):EBI-1139830

Protein

rpmB

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MIIRDENYFT DKYELTRTHS EVLEAVKVVK PGKTLDLGCG NGRNSLYLAA NGYDVDAWDK
NAMSIANVER IKSIENLDNL HTRVVDLNNL TFDRQYDFIL STVVLMFLEA KTIPGLIANM
QRCTKPGGYN LIVAAMDTAD YPCTVGFPFA FKEGELRRYY EGWERVKYNE DVGELHRTDA
NGNRIKLRFA TMLARKK
Length

197

Mol. Wt

22.531 kDa

pI

7.4 (calculated)

Extinction coefficient

25,900 - 26,275 (calc based on 10 Y, 2 W, and 3 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..193

PF03848 Tellurite resistance protein TehB

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tehB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129389

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945979

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004772

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P25397

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11884

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11884

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945979

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001814

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1830

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

4.94E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

11.537+/-0.115

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.049747049

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

1103

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

768

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

870

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

tehAB

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1499566..1499606 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1430 (EcoliWiki Page)

NCBI GEO profiles for tehB

microarray

GenExpDB:b1430 (EcoliWiki Page)

Summary of data for tehB from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to tehB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11884

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1830

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1430

EcoGene

EcoGene:EG11884

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001814

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004772

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Apis mellifera

  • ENSAPMP00000028172 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

TEHB

From SHIGELLACYC

E. coli O157

TEHB

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Panther (EcoliWiki Page)

PTHR10108:SF216

Superfamily (EcoliWiki Page)

SUPERFAMILY:53335

Pfam (EcoliWiki Page)

PF03848 Tellurite resistance protein TehB

EcoCyc

EcoCyc:EG11884

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11884

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001814

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1830

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004772

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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