tdcB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tdcB

Mnemonic

Threonine dehydratase, catabolic

Synonyms

ECK3106, b3117, JW3088, tdc[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

70.33 minutes 

MG1655: 3264050..3263061
<gbrowseImage> name=NC_000913:3263061..3264050 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3199062..3198073
<gbrowseImage> name=NC_012967:3198073..3199062 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3150209..3151198
<gbrowseImage> name=NC_012759:3150209..3151198 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3265883..3264894
<gbrowseImage> name=NC_007779:3264894..3265883 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3361795..3360806
<gbrowseImage> name=NC_010473:3360806..3361795 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3263061

Edman degradation

PMID:6751404[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔtdcB (Keio:JW3088)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10352[4]

tdcB::Tn5KAN-I-SceI (FB21028)

Insertion at nt 718 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB21028

contains pKD46

ΔtdcB733::kan

PMID:16738554[3]

CGSC:103112


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3088

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCCATATTACATACGATCTGCC

Primer 2:CCAGCGTCAACGAAACCGGTGAT

8F8

Kohara Phage

Genobase

PMID:3038334[7]

aer-3075::Tn10

Linked marker

CAG12152 = CGSC7438[4]

est. P1 cotransduction: 11% [8]
Synonyms:zgh-3075::Tn10, zgj-3075::Tn10

sfsB203::Tn10

Linked marker

CAG12072 = CGSC7440[4]

est. P1 cotransduction: 2% [8]
Synonyms:zgj-203::Tn10, zha-203::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10990

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10990

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000979

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947633

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0983

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010249

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Kim, SS & Datta, P (1982) Chemical characterization of biodegradative threonine dehydratases from two enteric bacteria. Biochim. Biophys. Acta 706 27-35 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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