tatE:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

tatE

Gene Synonym(s)

ECK0620, b0627, JW0622, ybeC[1], ybeC

Product Desc.

TatE[2][3];

Component of TatABCE protein export complex[2][3]

Protein translocase, Sec-independent; mediates export of folded and ligand-bound proteins[4]

Product Synonyms(s)

TatABCE protein translocation system subunit[1], B0627[2][1], YbeC[2][1], TatE[2][1] , ECK0620, JW0622, ybeC, b0627

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): tatE[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tatE

Mnemonic

Twin arginine translocation

Synonyms

ECK0620, b0627, JW0622, ybeC[1], ybeC

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

14.19 minutes 

MG1655: 658170..658373
<gbrowseImage> name=NC_000913:658170..658373 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 641530..641733
<gbrowseImage> name=NC_012967:641530..641733 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 560930..561133
<gbrowseImage> name=NC_012759:560930..561133 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 659369..659572
<gbrowseImage> name=NC_007779:659369..659572 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 597502..597705
<gbrowseImage> name=NC_010473:597502..597705 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 710762..710965
<gbrowseImage> name=NC_010473:710762..710965 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔtatE (Keio:JW0622)

deletion

deletion

PMID:16738554

Shigen
CGSC8729[5]

ΔtatE766::kan

PMID:16738554

CGSC:103098


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0622

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGGTGAGATTAGTATTACCAA

Primer 2:CCCTCTTTATGAGAGAGCTTTTC

1G6

Kohara Phage

Genobase

PMID:3038334

crcA280::Tn10

Linked marker

CAG12077 = CGSC7347[5]

est. P1 cotransduction: 88% [6]
Synonyms:zbe-280::Tn10

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[5]

est. P1 cotransduction: 16% [6]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11305

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11305

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001278

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945228

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1282

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002153

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

TatE

Synonyms

TatABCE protein translocation system subunit[1], B0627[2][1], YbeC[2][1], TatE[2][1] , ECK0620, JW0622, ybeC, b0627

Product description

TatE[2][3];

Component of TatABCE protein export complex[2][3]

Protein translocase, Sec-independent; mediates export of folded and ligand-bound proteins[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0009306

protein secretion

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00236

P

Seeded from EcoCyc (v14.0)

complete

GO:0009306

protein secretion

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006312

P

Seeded from EcoCyc (v14.0)

complete

GO:0015031

protein transport

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00236

P

Seeded from EcoCyc (v14.0)

complete

GO:0015031

protein transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003369

P

Seeded from EcoCyc (v14.0)

complete

GO:0015031

protein transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006312

P

Seeded from EcoCyc (v14.0)

complete

GO:0015031

protein transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0653

P

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006312

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0008320

protein transmembrane transporter activity

PMID:9649434

IGI: Inferred from Genetic Interaction

EcoliWiki:tatA


F

replaced original annotation to GO:0008565 because the term was obsoleted

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9904

C

Seeded from EcoCyc (v14.0)

complete

GO:0033281

TAT protein transport complex

PMID:9649434

IMP: Inferred from Mutant Phenotype

C

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of TatABCE protein export complex

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MGEISITKLL VVAALVVLLF GTKKLRTLGG DLGAAIKGFK KAMNDDDAAA KKGADVDLQA
EKLSHKE
Length

67

Mol. Wt

7.024 kDa

pI

9.5 (calculated)

Extinction coefficient

0 (calc based on 0 Y, 0 W, and 0 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

4..62

PF02416 mttA/Hcf106 family

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tatE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128610

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945228

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002153

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A843

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11305

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11305

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945228

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001278

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1282

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

27.157+/-0.209

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

Protein

E. coli K-12 MG1655

3465

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1286

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1614

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

tatE

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:658150..658190 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0627 (EcoliWiki Page)

NCBI GEO profiles for tatE

microarray

GenExpDB:b0627 (EcoliWiki Page)

Summary of data for tatE from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (657967..658216) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ13; Well:F9[7]

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Notes

Accessions Related to tatE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11305

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1282

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0627

EcoGene

EcoGene:EG11305

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001278

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002153

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000028218 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

YBEC

From SHIGELLACYC

E. coli O157

YBEC

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF02416 mttA/Hcf106 family

EcoCyc

EcoCyc:EG11305

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11305

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001278

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1282

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002153

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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