PMID:9649434
| Citation |
Sargent, F, Bogsch, EG, Stanley, NR, Wexler, M, Robinson, C, Berks, BC and Palmer, T (1998) Overlapping functions of components of a bacterial Sec-independent protein export pathway. EMBO J. 17:3640-50 |
|---|---|
| Abstract |
We describe the identification of two Escherichia coli genes required for the export of cofactor-containing periplasmic proteins, synthesized with signal peptides containing a twin arginine motif. Both gene products are homologous to the maize HCF106 protein required for the translocation of a subset of lumenal proteins across the thylakoid membrane. Disruption of either gene affects the export of a range of such proteins, and a complete block is observed when both genes are inactivated. The Sec protein export pathway was unaffected, indicating the involvement of the gene products in a novel export system. The accumulation of active cofactor-containing proteins in the cytoplasm of the mutant strains suggests a role for the gene products in the translocation of folded proteins. One of the two HCF106 homologues is encoded by the first gene of a four cistron operon, tatABCD, and the second by an unlinked gene, tatE. A mutation previously assigned to the hcf106 homologue encoded at the tatABCD locus, mttA, lies instead in the tatB gene. |
| Links |
PubMed PMC1170700 Online version:10.1093/emboj/17.13.3640 |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/physiology; Base Sequence; Biological Transport; Cloning, Molecular; Cytoplasm/metabolism; DNA, Bacterial; Enzyme Activation; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/physiology; Formate Dehydrogenases/metabolism; Genes, Bacterial; Hydrogenase/metabolism; Membrane Proteins/chemistry; Molecular Sequence Data; Mutagenesis; Oxidoreductases, N-Demethylating/genetics; Oxidoreductases, N-Demethylating/metabolism; Phenotype; Plant Proteins; Protein Precursors/metabolism; Protein Processing, Post-Translational; Recombinant Fusion Proteins/metabolism |
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