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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	tas
Gene Synonym(s)	ECK2830, b2834, JW2802, ygdS^[1], ygdS
Product Desc.	putative NAD(P)-linked reductase that acts in starvation-associated mutation^[2]^[3] Suppresses tyrosine requirement of tyrA14 O6 strain; probable aldo-keto reductase, NADP(H)-dependent^[4]
Product Synonyms(s)	predicted oxidoreductase, NADP(H)-dependent aldo-keto reductase^[1], B2834^[2]^[1], YgdS^[2]^[1], Tas^[2]^[1] , ECK2830, JW2802, ygdS, b2834
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): tas^[2]
Regulation/Activity
Quick Links
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Notes

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The natural substrate and physiological role for Tas is unknown. NADP(H) is found bound to the crystals, but no enzymatic activity for Tas has been demonstrated.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	tas
Mnemonic	Tyrosine auxotrophy suppressor
Synonyms	ECK2830, b2834, JW2802, ygdS^[1], ygdS
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	64 minutes	MG1655: 2969619..2970659 <gbrowseImage> name=NC_000913:2969619..2970659 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2866217..2867257 <gbrowseImage> name=NC_012967:2866217..2867257 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2856767..2857807 <gbrowseImage> name=NC_012759:2856767..2857807 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2970253..2971293 <gbrowseImage> name=NC_007779:2970253..2971293 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3063489..3064529 <gbrowseImage> name=NC_010473:3063489..3064529 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
Δtas (Keio:JW2802)	deletion	deletion	PMID:16738554	Shigen CGSC10189^[5]
tas-1(del-ins)::kan			PMID:9560382	CGSC:64368
Δtas-759::kan			PMID:16738554	CGSC:103090
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2802	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCAATATCACCGTATACCCCA Primer 2:CCTGGTGCCGGATAAGTATAAAC
5A10	Kohara Phage	Genobase	PMID:3038334
8H3	Kohara Phage	Genobase	PMID:3038334
recD1901::Tn10	Linked marker	CAG12135 = CGSC7429^[5]	est. P1 cotransduction: 51% ^[6]
speA210::Tn10	Linked marker	CAG12168 = CGSC7431^[5]	est. P1 cotransduction: % ^[6] Synonyms:zgd-210::Tn10, zgf-210::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7462	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13093	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004025	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947306	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2898	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009298	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Tas
Synonyms	predicted oxidoreductase, NADP(H)-dependent aldo-keto reductase^[1], B2834^[2]^[1], YgdS^[2]^[1], Tas^[2]^[1] , ECK2830, JW2802, ygdS, b2834
Product description	putative NAD(P)-linked reductase that acts in starvation-associated mutation^[2]^[3] Suppresses tyrosine requirement of tyrA14 O6 strain; probable aldo-keto reductase, NADP(H)-dependent^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0016491	oxidoreductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001395	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020471	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001395	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020471	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
	GO:0034198	cellular response to amino acid starvation	PMID:9560382	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0004033	aldo-keto reductase activity	PMID:14517983	TAS: Traceable Author Statement		F		complete
	GO:0005575	cellular component	127.0.0.1 14:56, 17 February 2015 (CST)	ND: No biological Data available		C		Missing: reference
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	groL	PMID:16606699	Experiment(s):EBI-1144115
Protein	otsB	PMID:16606699	Experiment(s):EBI-1144115
Protein	mnmG	PMID:19402753	LCMS(ID Probability):99.6
Protein	pbpG	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MQYHRIPHSS LEVSTLGLGT MTFGEQNSEA DAHAQLDYAV AQGINLIDVA EMYPVPPRPE TQGLTETYVG NWLAKHGSRE KLIIASKVSG PSRNNDKGIR PDQALDRKNI REALHDSLKR LQTDYLDLYQ VHWPQRPTNC FGKLGYSWTD SAPAVSLLDT LDALAEYQRA GKIRYIGVSN ETAFGVMRYL HLADKHDLPR IVTIQNPYSL LNRSFEVGLA EVSQYEGVEL LAYSCLGFGT LTGKYLNGAK PAGARNTLFS RFTRYSGEQT QKAVAAYVDI ARRHGLDPAQ MALAFVRRQP FVASTLLGAT TMDQLKTNIE SLHLELSEDV LAEIEAVHQV YTYPAP
Length	346
Mol. Wt	38.499 kDa
pI	6.7 (calculated)
Extinction coefficient	43,320 - 43,570 (calc based on 18 Y, 3 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	15..339	PF00248 Aldo/keto reductase family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tas taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130738	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947306	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009298	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A9T4	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7462	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13093	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947306	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004025	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2898	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.89E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	882	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	872	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	942	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
tas
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2969599..2969639 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2834 (EcoliWiki Page)	NCBI GEO profiles for tas
microarray	GenExpDB:b2834 (EcoliWiki Page)	Summary of data for tas from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to tas Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7462	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2898	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2834
EcoGene	EcoGene:EG13093	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004025	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009298	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT1G04420 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q75H72 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	S3042	From SHIGELLACYC
E. coli O157	Z4152	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00248 Aldo/keto reductase family
Superfamily (EcoliWiki Page)	SUPERFAMILY:51430
Panther (EcoliWiki Page)	PTHR11732:SF115
EcoCyc	EcoCyc:G7462	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13093	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004025	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2898	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009298	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

tas:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to tas Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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