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Gene |

Product(s) |

Expression|

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Standard Name	sucA
Gene Synonym(s)	ECK0714, b0726, JW0715, lys, met, lys+met^[1]
Product Desc.	2-oxoglutarate dehydrogenase, E1 component; yields succinyl-CoA and CO(2)^[2]
Product Synonyms(s)	2-oxoglutarate decarboxylase, thiamin-requiring^[1] , ECK0714, JW0715, lys, b0726
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): sdhCDAB-sucABCD^[3], OP00142, sdhCDAB, sucABCD^[3], OP00143, suc
Regulation/Activity
Quick Links
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Notes

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sucAB is a synthetic lethal with sucCD.^[2]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	sucA
Mnemonic	Succinate
Synonyms	ECK0714, b0726, JW0715, lys, met, lys+met^[1]
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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	16.34 minutes	MG1655: 757929..760730 <gbrowseImage> name=NC_000913:757929..760730 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 739497..742298 <gbrowseImage> name=NC_012967:739497..742298 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 660689..663490 <gbrowseImage> name=NC_012759:660689..663490 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 759128..761929 <gbrowseImage> name=NC_007779:759128..761929 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 810521..813322 <gbrowseImage> name=NC_010473:810521..813322 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
ΔsucA (Keio:JW0715)	deletion	deletion			PMID:16738554	Shigen CGSC8786^[4]
ΔsucA::kan	deletion		Biolog:respiration	unable to respire Dextrin	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire D-Galactose	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire b-Methyl-D-glucoside	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire a-D-Glucose-1-phosphate	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire DL-a-Glycerol	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire D-Galacturonate	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire D-Glucuronate	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire a-Hydroxybutyrate	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire a-Ketobutyrate	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire a-Ketoglutarate	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire DL-Lactate	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire Succinate	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire Bromosuccinate	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire Glucuronamide	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire L-Alanine	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire L-Alanyl-glycine	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire L-Asparagine	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire L-Aspartate	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire Glycyl-L-aspartate	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire L-Serine	PMID:16095938
ΔsucA::kan	deletion		Biolog:respiration	unable to respire Uridine	PMID:16095938
sucA8						CGSC:11091
ΔsucA775::kan					PMID:16738554	CGSC:103050
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0715	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCAGAACAGCGCTTTGAAAGC Primer 2:CCTTCGACGTTCAGCGCGTCATT
7E10	Kohara Phage	Genobase	PMID:3038334
18F11	Kohara Phage	Genobase	PMID:3038334
asnB3057::Tn10	Linked marker	CAG18433 = CGSC7349^[4]	est. P1 cotransduction: 6% ^[5] Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.
nadA57::Tn10	Linked marker	CAG12147 = CGSC7351^[4]	est. P1 cotransduction: 45% ^[5]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10979	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10979	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000968	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945303	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0972	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002478	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	SucA
Synonyms	2-oxoglutarate decarboxylase, thiamin-requiring^[1] , ECK0714, JW0715, lys, b0726
Product description	2-oxoglutarate dehydrogenase, E1 component; yields succinyl-CoA and CO(2)^[2]
EC number (for enzymes)	1.2.4.2^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0006099	tricarboxylic acid cycle				P	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0004591	oxoglutarate dehydrogenase (succinyl-transferring) activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011603	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004591	oxoglutarate dehydrogenase (succinyl-transferring) activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.2.4.2	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005829	cytosol	PMID:16858726^[6]	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0006096	glycolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011603	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006096	glycolysis	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0324	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001017	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016624	oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001017	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030976	thiamin pyrophosphate binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011603	F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	hflK		PMID:15690043^[7]	Experiment(s):EBI-880717
Protein	lipA		PMID:15690043^[7]	Experiment(s):EBI-880717
Protein	lpdA		PMID:15690043^[7]	Experiment(s):EBI-880717, EBI-886788
Protein	sucB		PMID:15690043^[7]	Experiment(s):EBI-880717, EBI-880739, EBI-886788
Protein	groL		PMID:16606699^[8]	Experiment(s):EBI-1137802
Protein	dnaE		PMID:16606699^[8]	Experiment(s):EBI-1137802
Protein	oxc		PMID:16606699^[8]	Experiment(s):EBI-1137802
Protein	rplC		PMID:15690043^[7]	Experiment(s):EBI-886788
Protein	lipA		PMID:19402753^[9]	MALDI(Z-score):25.667279
Protein	sucB		PMID:19402753^[9]	LCMS(ID Probability):99.6 MALDI(Z-score):33.552055
Small Molecule	TTTPP	selectively inhibits pyruvate or 2-oxoglutarate dehydrogenase (El).
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	MRITIILVAP ARAENIGAAA RAMKTMGFSD LRIVDSQAHL EPATRWVAHG SGDIIDNIKV FPTLAESLHD VDFTVATTAR SRAKYHYYAT PVELVPLLEE KSSWMSHAAL VFGREDSGLT NEELALADVL TGVPMVADYP SLNLGQAVMV YCYQLATLIQ QPAKSDATAD QHQLQALRER AMTLLTTLAV ADDIKLVDWL QQRLGLLEQR DTAMLHRLLH DIEKNITK
Length	228
Mol. Wt	25.259 kDa
pI	5.9 (calculated)
Extinction coefficient	25,440 - 25,565 (calc based on 6 Y, 3 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	589..785	PF02779 Transketolase, pyrimidine binding domain	PMID:19920124^[10]
Domain	207..516	PF00676 Dehydrogenase E1 component	PMID:19920124^[10]
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<motif_map/>

Structure
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Structures

<beststructure> gene=sucA taxon=562,83333 </beststructure>

Models

View models at:

PSI-PRED (P0AFG3)
Genomes TO Protein (P0AFG3) (GTOP)

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128701	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945303	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002478	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AFG3	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10979	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10979	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945303	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000968	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0972	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.33E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	158.495+/-0.802	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.211353088	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	3431	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	2121	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	2158	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
sdhCDAB-sucABCD
sucABCD
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Notes

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Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:757909..757949 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

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Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0726 (EcoliWiki Page)	NCBI GEO profiles for sucA
microarray	GenExpDB:b0726 (EcoliWiki Page)	Summary of data for sucA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to sucA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10979	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0972	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0726
EcoGene	EcoGene:EG10979	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000968	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002478	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Shigella flexneri	SUCA	From SHIGELLACYC
E. coli O157	SUCA	From ECOO157CYC
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Notes

Families

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See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02779 Transketolase, pyrimidine binding domain
Pfam (EcoliWiki Page)	PF00676 Dehydrogenase E1 component
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
↑ ^7.0 ^7.1 ^7.2 ^7.3 ^7.4 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
↑ ^8.0 ^8.1 ^8.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
↑ ^9.0 ^9.1 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
↑ ^10.0 ^10.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

sucA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to sucA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Notes

Families

Notes

Links

References

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