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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	slmA
Gene Synonym(s)	ttk, ECK3631, b3641, JW5641, yicB^[1]^[2], yicB
Product Desc.	cell division inhibitor^[2]^[3] Division inhibitor that binds FtsZ and blocks septal rings; DNA-associated^[4]
Product Synonyms(s)	division inhibitor^[1], B3641^[2]^[1], YicB^[2]^[1], Ttk^[2]^[1], SlmA^[2]^[1] , ECK3631, JW5641, ttk, yicB, b3641
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): dut-slmA^[2]
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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TetR family.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	slmA
Mnemonic	Synthetic lethal with min
Synonyms	ttk, ECK3631, b3641, JW5641, yicB^[1]^[2], yicB
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	82.17 minutes	MG1655: 3812517..3813113 <gbrowseImage> name=NC_000913:3812517..3813113 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3752849..3753445 <gbrowseImage> name=NC_012967:3752849..3753445 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3700843..3701439 <gbrowseImage> name=NC_012759:3700843..3701439 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3825921..3825325 <gbrowseImage> name=NC_007779:3825325..3825921 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	Reference	Availability	Comments
ttk::Tn5KAN-I-SceI (FB21357)	Insertion at nt 315 in Minus orientation	PMID:15262929	E. coli Genome Project:FB21357	does not contain pKD46
ttk::Tn5KAN-I-SceI (FB21358)	Insertion at nt 315 in Minus orientation	PMID:15262929	E. coli Genome Project:FB21358	contains pKD46
ΔslmA747::kan		PMID:16738554	CGSC:106346
slmA:::EZTn kan		PMID: 15916962
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID:16769691 Status: Primer 1: Primer 2:
2A6	Kohara Phage	Genobase	PMID:3038334
4D2	Kohara Phage	Genobase	PMID:3038334
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11191	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11191	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001172	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948158	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1177	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011902	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	SlmA
Synonyms	division inhibitor^[1], B3641^[2]^[1], YicB^[2]^[1], Ttk^[2]^[1], SlmA^[2]^[1] , ECK3631, JW5641, ttk, yicB, b3641
Product description	cell division inhibitor^[2]^[3] Division inhibitor that binds FtsZ and blocks septal rings; DNA-associated^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000917	barrier septum formation	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01839	P	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01839	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012287	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003700	transcription factor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001647	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01839	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006350	transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0804	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001647	P	Seeded from EcoCyc (v14.0)	complete
	GO:0007049	cell cycle	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0131	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009295	nucleoid	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0187	C	Seeded from EcoCyc (v14.0)	complete
	GO:0030528	transcription regulator activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011075	F	Seeded from EcoCyc (v14.0)	complete
	GO:0045449	regulation of transcription	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011075	P	Seeded from EcoCyc (v14.0)	complete
	GO:0045449	regulation of transcription	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012287	P	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	PMID:15916962	IDA: Inferred from Direct Assay		F	fluorescence microscopy- colocalization with DNA	complete
	GO:0045449	regulation of transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0805	P	Seeded from EcoCyc (v14.0)	complete
	GO:0051301	cell division	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0132	P	Seeded from EcoCyc (v14.0)	complete
	GO:0010974	negative regulation of barrier septum formation	PMID:21321206	IDA: Inferred from Direct Assay		P	Figure 3 and S3- SlmA destabilizes FtsZ polymers	complete
	GO:0043547	positive regulation of GTPase activity	PMID:21321206	IDA: Inferred from Direct Assay		P	Figure 4A- SlmA bound to consensus DNA increases FtsZ's GTPase activity	complete
	GO:0003677	DNA binding	PMID:21321206	IDA: Inferred from Direct Assay		F	Figure S7- EMSA	complete
	GO:0032272	negative regulation of protein polymerization	PMID:21321206	IDA: Inferred from Direct Assay		P	Figures 1, 3 and S3- interferes with FtsZ polymerization	complete
	GO:0000918	barrier septum formation	PMID:15916962	IMP: Inferred from Mutant Phenotype		P	Figure 3	complete
	GO:0010974	cell division	PMID:15916962	IGI: Inferred from Genetic Interaction	EcoliWiki:minB	P	Figure 1 and 2	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	FtsZ		PMID:21321206	Bacterial 2 hybrid
Protein	FtsZ		PMID:15916962
DNA	SlmA binding sequence	consensus binding dramatically enhances SlmA's ability to interfere with FtsZ polymerization	PMID:21321206	Figure 2 and S7- EMSA
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Nucleoid		Fluorescence microscopy	PMID:15916962
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MPPGKCLFSG VFCNMAEKQT AKRNRREEIL QSLALMLESS DGSQRITTAK LAASVGVSEA ALYRHFPSKT RMFDSLIEFI EDSLITRINL ILKDEKDTTA RLRLIVLLLL GFGERNPGLT RILTGHALMF EQDRLQGRIN QLFERIEAQL RQVLREKRMR EGEGYTTDET LLASQILAFC EGMLSRFVRS EFKYRPTDDF DARWPLIAAQ LQ
Length	212
Mol. Wt	24.319 kDa
pI	8.8 (calculated)
Extinction coefficient	9,970 - 10,345 (calc based on 3 Y, 1 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	16..62	PF00440 Bacterial regulatory proteins, tetR family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=slmA taxon=562,83333 </beststructure>

SlmA-FtsZ structure

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
Purified protein	SlmA R73D- aberrant FtsZ binding	PMID:21321206
Purified protein	SlmA T33A- aberrant DNA binding	PMID:21321206
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111625	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948158	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011902	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0C093	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11191	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11191	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948158	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001172	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1177	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	2323	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	568	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	952	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
dut-slmA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3812497..3812537 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3641 (EcoliWiki Page)	NCBI GEO profiles for slmA
microarray	GenExpDB:b3641 (EcoliWiki Page)	Summary of data for slmA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to slmA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11191	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1177	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3641
EcoGene	EcoGene:EG11191	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001172	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011902	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	TTK	From SHIGELLACYC
E. coli O157	TTK	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00440 Bacterial regulatory proteins, tetR family
Superfamily (EcoliWiki Page)	SUPERFAMILY:46689
Superfamily (EcoliWiki Page)	SUPERFAMILY:48498
EcoCyc	EcoCyc:EG11191	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11191	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001172	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1177	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011902	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.

slmA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to slmA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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