slmA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

SlmA

Synonyms

division inhibitor[1], B3641[2][1], YicB[2][1], Ttk[2][1], SlmA[2][1] , ECK3631, JW5641, ttk, yicB, b3641

Product description

cell division inhibitor[2][3]

Division inhibitor that binds FtsZ and blocks septal rings; DNA-associated[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000917

barrier septum formation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01839

P

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01839

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012287

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001647

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01839

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001647

P

Seeded from EcoCyc (v14.0)

complete

GO:0007049

cell cycle

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0131

P

Seeded from EcoCyc (v14.0)

complete

GO:0009295

nucleoid

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0187

C

Seeded from EcoCyc (v14.0)

complete

GO:0030528

transcription regulator activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011075

F

Seeded from EcoCyc (v14.0)

complete

GO:0045449

regulation of transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011075

P

Seeded from EcoCyc (v14.0)

complete

GO:0045449

regulation of transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012287

P

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

PMID:15916962[5]

IDA: Inferred from Direct Assay

F

fluorescence microscopy- colocalization with DNA

complete

GO:0045449

regulation of transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0805

P

Seeded from EcoCyc (v14.0)

complete

GO:0051301

cell division

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0132

P

Seeded from EcoCyc (v14.0)

complete

GO:0010974

negative regulation of barrier septum formation

PMID:21321206[6]

IDA: Inferred from Direct Assay

P

Figure 3 and S3- SlmA destabilizes FtsZ polymers

complete

GO:0043547

positive regulation of GTPase activity

PMID:21321206[6]

IDA: Inferred from Direct Assay

P

Figure 4A- SlmA bound to consensus DNA increases FtsZ's GTPase activity

complete

GO:0003677

DNA binding

PMID:21321206[6]

IDA: Inferred from Direct Assay

F

Figure S7- EMSA

complete

GO:0032272

negative regulation of protein polymerization

PMID:21321206[6]

IDA: Inferred from Direct Assay

P

Figures 1, 3 and S3- interferes with FtsZ polymerization

complete

GO:0000918

barrier septum formation

PMID:15916962[5]

IMP: Inferred from Mutant Phenotype

P

Figure 3

complete

GO:0010974

cell division

PMID:15916962[5]

IGI: Inferred from Genetic Interaction

EcoliWiki:minB


P

Figure 1 and 2

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

FtsZ

PMID:21321206[6]

Bacterial 2 hybrid

Protein

FtsZ

PMID:15916962[5]

DNA

SlmA binding sequence

consensus binding dramatically enhances SlmA's ability to interfere with FtsZ polymerization

PMID:21321206[6]

Figure 2 and S7- EMSA

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Nucleoid

Fluorescence microscopy

PMID:15916962[5]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MPPGKCLFSG VFCNMAEKQT AKRNRREEIL QSLALMLESS DGSQRITTAK LAASVGVSEA
ALYRHFPSKT RMFDSLIEFI EDSLITRINL ILKDEKDTTA RLRLIVLLLL GFGERNPGLT
RILTGHALMF EQDRLQGRIN QLFERIEAQL RQVLREKRMR EGEGYTTDET LLASQILAFC
EGMLSRFVRS EFKYRPTDDF DARWPLIAAQ LQ
Length

212

Mol. Wt

24.319 kDa

pI

8.8 (calculated)

Extinction coefficient

9,970 - 10,345 (calc based on 3 Y, 1 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

16..62

PF00440 Bacterial regulatory proteins, tetR family

PMID:19920124[7]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=slmA taxon=562,83333 </beststructure>

SlmA-FtsZ structure

Models

View models at:

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

Purified protein

SlmA R73D- aberrant FtsZ binding

PMID:21321206[6]

Purified protein

SlmA T33A- aberrant DNA binding

PMID:21321206[6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111625

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948158

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011902

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0C093

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11191

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11191

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948158

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001172

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1177

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 Bernhardt, TG & de Boer, PA (2005) SlmA, a nucleoid-associated, FtsZ binding protein required for blocking septal ring assembly over Chromosomes in E. coli. Mol. Cell 18 555-64 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 Cho, H et al. (2011) Nucleoid occlusion factor SlmA is a DNA-activated FtsZ polymerization antagonist. Proc. Natl. Acad. Sci. U.S.A. 108 3773-8 PubMed
  7. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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