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Expression|

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Standard Name	secG
Gene Synonym(s)	ECK3164, b3175, JW3142, prlH^[1], prlH
Product Desc.	SecG^[2]^[3]; Component of SecEGY-Secretion Complex^[2]^[3]; Sec Protein Secretion Complex^[2]^[3] SecG inner membrane secretion protein; complexes with and assists the SecYE core translocon to interact with SecA to export proteins^[4]
Product Synonyms(s)	preprotein translocase membrane subunit^[1], EG12095^[2]^[1], B3175^[2]^[1], PrlH^[2]^[1], SecG^[2]^[1] , ECK3164, JW3142, prlH, b3175
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s):
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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SecYEG complex interacts with SecDFyajC to form a hexameric holocomplex. N-terminus is blocked.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	secG
Mnemonic	Secretory
Synonyms	ECK3164, b3175, JW3142, prlH^[1], prlH
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	71.56 minutes	MG1655: 3320527..3320195 <gbrowseImage> name=NC_000913:3320195..3320527 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3257739..3257407 <gbrowseImage> name=NC_012967:3257407..3257739 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3207343..3207675 <gbrowseImage> name=NC_012759:3207343..3207675 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3322360..3322028 <gbrowseImage> name=NC_007779:3322028..3322360 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3418272..3417940 <gbrowseImage> name=NC_010473:3417940..3418272 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔsecG (Keio:JW3142)	deletion	deletion		PMID:16738554	Shigen CGSC10387^[5]
secG::Tn5KAN-I-SceI (FB21057)	Insertion at nt 95 in Plus orientation			PMID:15262929	E. coli Genome Project:FB21057	contains pKD46
secG::Tn5KAN-I-SceI (FB21058)	Insertion at nt 95 in Plus orientation			PMID:15262929	E. coli Genome Project:FB21058	does not contain pKD46
secGT41P		T41P	Affects activity			seeded from UniProt:P0AG99
secGL42P		L42P	Affects activity			seeded from UniProt:P0AG99
secGF43S,Y		F43S,Y	Affects activity			seeded from UniProt:P0AG99
ΔsecG783::kan				PMID:16738554	CGSC:102904
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3142	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTATGAAGCTCTTTTAGTAGT Primer 2:CCGTTCGGGATATCGCTGGTCGG
E4G11S	Kohara Phage	Genobase	PMID:3038334
18H7	Kohara Phage	Genobase	PMID:3038334
14F11	Kohara Phage	Genobase	PMID:3038334
aer-3075::Tn10	Linked marker	CAG12152 = CGSC7438^[5]	est. P1 cotransduction: % ^[6] Synonyms:zgh-3075::Tn10, zgj-3075::Tn10
sfsB203::Tn10	Linked marker	CAG12072 = CGSC7440^[5]	est. P1 cotransduction: 68% ^[6] Synonyms:zgj-203::Tn10, zha-203::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12095	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12095	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002000	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947720	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2019	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010436	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	SecG
Synonyms	preprotein translocase membrane subunit^[1], EG12095^[2]^[1], B3175^[2]^[1], PrlH^[2]^[1], SecG^[2]^[1] , ECK3164, JW3142, prlH, b3175
Product description	SecG^[2]^[3]; Component of SecEGY-Secretion Complex^[2]^[3]; Sec Protein Secretion Complex^[2]^[3] SecG inner membrane secretion protein; complexes with and assists the SecYE core translocon to interact with SecA to export proteins^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009306	protein secretion	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004692	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015450	P-P-bond-hydrolysis-driven protein transmembrane transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004692	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004692	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	PMID:7650029	IDA: Inferred from Direct Assay		C		complete
	GO:0043952	protein transport by the Sec complex	PMID:7650029	IDA: Inferred from Direct Assay		P		complete
	GO:0065002	intracellular protein transmembrane transport	PMID:7650029	IDA: Inferred from Direct Assay		P		complete
	GO:0006886	intracellular protein transport	PMID:7650029	IDA: Inferred from Direct Assay		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References
Protein	Subunits of SecEGY-Secretion Complex	could be indirect	PMID:7650029
Protein	Subunits of Sec Protein Secretion Complex	could be indirect	PMID:7650029
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane	C-terminus localized to the periplasm with 3 predicted transmembrane domains	Daley et al. (2005) ^[7]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MYEALLVVFL IVAIGLVGLI MLQQGKGADM GASFGAGASA TLFGSSGSGN FMTRMTALLA TLFFIISLVL GNINSNKTNK GSEWENLSAP AKTEQTQPAA PAKPTSDIPN
Length	110
Mol. Wt	11.365 kDa
pI	6.3 (calculated)
Extinction coefficient	6,990 (calc based on 1 Y, 1 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	2..75	PF03840 Preprotein translocase SecG subunit		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=secG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131065	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947720	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010436	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AG99	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12095	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12095	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947720	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002000	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2019	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	12154	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	2964	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	4058	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3320507..3320547 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3175 (EcoliWiki Page)	NCBI GEO profiles for secG
microarray	GenExpDB:b3175 (EcoliWiki Page)	Summary of data for secG from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to secG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12095	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2019	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3175
EcoGene	EcoGene:EG12095	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002000	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010436	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000049318 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	SECG	From SHIGELLACYC
E. coli O157	SECG	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03840 Preprotein translocase SecG subunit
EcoCyc	EcoCyc:EG12095	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12095	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002000	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2019	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010436	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

secG:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to secG Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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