sdhA:Gene

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

<protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

sdhA

Mnemonic

Succinate dehydrogenase

Synonyms

ECK0712, b0723, JW0713, dhsA[1]

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

16.28 minutes 

MG1655: 755130..756896
<gbrowseImage> name=NC_000913:755130..756896 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 736698..738464
<gbrowseImage> name=NC_012967:736698..738464 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 657890..659656
<gbrowseImage> name=NC_012759:657890..659656 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 756329..758095
<gbrowseImage> name=NC_007779:756329..758095 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 807722..809488
<gbrowseImage> name=NC_010473:807722..809488 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

755130

Edman degradation

PMID:9298646[2]
PMID:9600841[3]
PMID:9740056[4]


<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔsdhA (Keio:JW0713)

deletion

deletion

PMID:16738554[5]

Shigen
CGSC8302[6]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire D-Galactose

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire b-Methyl-D-glucoside

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire DL-a-Glycerol

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire D-Galacturonate

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire D-Glucuronate

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire DL-Lactate

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire Glucuronamide

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[7]

ΔsdhA::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938[7]

ΔsdhA773::kan

PMID:16738554[5]

CGSC:99833


<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0713

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCAAATTGCCAGTCAGAGAATT

Primer 2:CCGTAAGTACGAATCTTCGGCGG

7E10

Kohara Phage

Genobase

PMID:3038334[9]

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[6]

est. P1 cotransduction: 7% [10]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[6]

est. P1 cotransduction: 40% [10]

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10931

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10931

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000920

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945402

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0924

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002466

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  3. Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
  4. Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
  5. 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
  7. 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 7.16 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories