rssB:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
rssB |
|---|---|
| Mnemonic |
Regulation of Sigma S |
| Synonyms |
ECK1230, b1235, JW1223, hnr, mviA, sprE, ychI, ychL[1], verA, ychL |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
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MG1655 |
27.79 minutes |
MG1655: 1289465..1290478 |
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NC_012967: 1290036..1291049 |
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NC_012759: 1179050..1179976 |
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W3110 |
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W3110: 1291819..1292832 |
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DH10B: 1329706..1330719 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔrssB (Keio:JW1223) |
deletion |
deletion |
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ΔsprE::kan |
deletion |
Biolog:respiration |
unable to respire D-Galactoniate |
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ΔsprE::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
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ΔsprE::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
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ΔsprE::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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ΔsprE::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
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ΔsprE::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
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ΔsprE::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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ΔsprE::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
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ΔsprE::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
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ΔrssB1273 |
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ΔrssB744::kan |
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overproduction |
Growth Phenotype |
toxic |
Suppressed by pcnA and hfq mutants PMID:19767441[5] | ||||
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CAG45114 rssB::Tet |
insertion |
SigmaE activity |
Increase in sigmaE activity |
Paternal Strain: CAG45114 Experimental Strain: SMR15271 |
See table S11 for full experimental data. | ||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1223 |
Plasmid clone |
Status:Clone OK Primer 1:GCCACGCAGCCATTGGTCGGAAA Primer 2:CCTTCTGCAGACAACATCAAGCG | |
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Kohara Phage |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 7% [9] | ||
|
Linked marker |
est. P1 cotransduction: 61% [9] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Carabetta, VJ et al. (2009) The response regulator SprE (RssB) modulates polyadenylation and mRNA stability in Escherichia coli. J. Bacteriol. 191 6812-21 PubMed
- ↑ Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 8.0 8.1 8.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
