rsgA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

rsgA

Gene Synonym(s)

ECK4157, b4161, JW4122, yjeQ[1], engC, yjeQ

Product Desc.

ribosome small subunit-dependent GTPase A[2][3]

Ribosome-stimulated GTPase, function unknown; low adundance protein; putative RNA binding protein[4]

Product Synonyms(s)

ribosome small subunit-dependent GTPase A[1], B4161[2][1], YjeQ[2][1], RsgA[2][1] , ECK4157, engC, JW4122, yjeQ, b4161

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): rsgA[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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rsgA(yjeQ) has been reported to be an essential gene, but deletion mutants have also been reported to grow slowly. RsgA:ribosomes is 1:200 in vivo. Guanine nucleotide-dependent 30S subunit binding greatly stimulates GTPase activity. OtherPDB (S.typhimurium):2RCN. RsgA may be involved in ribosome biogenesis.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rsgA

Mnemonic

Ribosome small subunit-dependent GTPase A

Synonyms

ECK4157, b4161, JW4122, yjeQ[1], engC, yjeQ

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

94.59 minutes 

MG1655: 4389532..4388480
<gbrowseImage> name=NC_000913:4388480..4389532 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4368612..4369664
<gbrowseImage> name=NC_012967:4368612..4369664 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4327215..4328267
<gbrowseImage> name=NC_012759:4327215..4328267 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4396189..4395137
<gbrowseImage> name=NC_007779:4395137..4396189 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4489894..4488842
<gbrowseImage> name=NC_010473:4488842..4489894 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrsgA (Keio:JW4122)

deletion

deletion

PMID:16738554

Shigen
CGSC10968[5]

rsgAS221A

S221A

Reduction in GTPase activity. Strong reduction; when associated with A- 220

seeded from UniProt:P39286

rsgAK220A

K220A

Reduction in GTPase activity. Strong reduction; when associated with A- 221

seeded from UniProt:P39286

ΔrsgA787::kan

PMID:16738554

CGSC:102814


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4122

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGTAAAAATAAACTCTCCAA

Primer 2:CCGTCATCCGTATCAGAAAAGTT

3H6

Kohara Phage

Genobase

PMID:3038334

3A1

Kohara Phage

Genobase

PMID:3038334

cadB2231::Tn10

Linked marker

CAG18488 = CGSC7479[5]

est. P1 cotransduction: 28% [6]
Synonyms:zjd-2231::Tn10

cycA30::Tn10

Linked marker

CAG12073 = CGSC7482[5]

est. P1 cotransduction: 21% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7841

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12479

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004337

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948674

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2372

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013626

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RsgA

Synonyms

ribosome small subunit-dependent GTPase A[1], B4161[2][1], YjeQ[2][1], RsgA[2][1] , ECK4157, engC, JW4122, yjeQ, b4161

Product description

ribosome small subunit-dependent GTPase A[2][3]

Ribosome-stimulated GTPase, function unknown; low adundance protein; putative RNA binding protein[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0003924

GTPase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01820

F

Seeded from EcoCyc (v14.0)

complete

GO:0003924

GTPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004881

F

Seeded from EcoCyc (v14.0)

complete

GO:0003924

GTPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010914

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:16606699

IPI: Inferred from Physical Interaction

UniProtKB:P23524

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01820

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004881

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010914

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0342

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

aceE

PMID:15690043

Experiment(s):EBI-886442

Protein

eutA

PMID:15690043

Experiment(s):EBI-886442

Protein

dnaK

PMID:15690043

Experiment(s):EBI-886442

Protein

fusA

PMID:15690043

Experiment(s):EBI-886442

Protein

gapA

PMID:15690043

Experiment(s):EBI-886442

Protein

groL

PMID:15690043

Experiment(s):EBI-886442

Protein

rplC

PMID:15690043

Experiment(s):EBI-886442

Protein

rplD

PMID:15690043

Experiment(s):EBI-886442

Protein

rplL

PMID:15690043

Experiment(s):EBI-886442

Protein

rplM

PMID:15690043

Experiment(s):EBI-886442

Protein

rplV

PMID:15690043

Experiment(s):EBI-886442

Protein

rplW

PMID:15690043

Experiment(s):EBI-886442

Protein

rpmG

PMID:15690043

Experiment(s):EBI-886442

Protein

rpsA

PMID:15690043

Experiment(s):EBI-886442

Protein

rpsB

PMID:15690043

Experiment(s):EBI-886442

Protein

rpsC

PMID:15690043

Experiment(s):EBI-886442

Protein

rpsG

PMID:15690043

Experiment(s):EBI-886442

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-886442

Protein

rpsM

PMID:15690043

Experiment(s):EBI-886442

Protein

rpsN

PMID:15690043

Experiment(s):EBI-886442

Protein

rpsP

PMID:15690043

Experiment(s):EBI-886442

Protein

tufA

PMID:15690043

Experiment(s):EBI-886442

Protein

wcaJ

PMID:15690043

Experiment(s):EBI-886442

Protein

yaiW

PMID:15690043

Experiment(s):EBI-886442

Protein

ybaL

PMID:15690043

Experiment(s):EBI-886442

Protein

ybeR

PMID:15690043

Experiment(s):EBI-886442

Protein

ydaY

PMID:15690043

Experiment(s):EBI-886442

Protein

ydiA

PMID:15690043

Experiment(s):EBI-886442

Protein

pabB

PMID:15690043

Experiment(s):EBI-886442

Protein

garK

PMID:16606699

Experiment(s):EBI-1147594

Protein

groS

PMID:19402753

LCMS(ID Probability):99.6

Protein

gapA

PMID:19402753

LCMS(ID Probability):99.6

Protein

groL

PMID:19402753

LCMS(ID Probability):99.6

Protein

fusA

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpmG

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplW

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplD

PMID:19402753

LCMS(ID Probability):99.6

Protein

ppsR

PMID:19402753

LCMS(ID Probability):99.0

Protein

ybaL

PMID:19402753

LCMS(ID Probability):99.0

Protein

eutA

PMID:19402753

LCMS(ID Probability):99.0

Protein

pabB

PMID:19402753

LCMS(ID Probability):99.0

Protein

wcaJ

PMID:19402753

LCMS(ID Probability):99.0

Protein

ybeR

PMID:19402753

LCMS(ID Probability):99.0

Protein

yaiW

PMID:19402753

LCMS(ID Probability):99.0

Protein

rplL

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MNANHQRRLK TSKEKPDYDD NLFGEPDEGI VISRFGMHAD VESADGDVHR CNIRRTIRSL
VTGDRVVWRP GKPAAEGVNV KGIVEAVHER TSVLTRPDFY DGVKPIAANI DQIVIVSAIL
PELSLNIIDR YLVACETLQI EPIIVLNKID LLDDEGMAFV NEQMDIYRNI GYRVLMVSSH
TQDGLKPLEE ALTGRISIFA GQSGVGKSSL LNALLGLQKE ILTNDISDNS GLGQHTTTAA
RLYHFPHGGD VIDSPGVREF GLWHLEPEQI TQGFVEFHDY LGLCKYRDCK HDTDPGCAIR
EAVEEGKIAE TRFENYHRIL ESMAQVKTRK NFSDTDD
Length

337

Mol. Wt

37.682 kDa

pI

5.2 (calculated)

Extinction coefficient

24,410 - 25,035 (calc based on 9 Y, 2 W, and 5 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

173..334

PF03193 Protein of unknown function, DUF258

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rsgA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111696

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948674

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013626

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P39286

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G7841

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12479

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948674

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004337

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2372

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

19.643+/-0.097

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

Protein

E. coli K-12 MG1655

1289

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

331

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

887

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

rsgA

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4389512..4389552 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b4161 (EcoliWiki Page)

NCBI GEO profiles for rsgA

microarray

GenExpDB:b4161 (EcoliWiki Page)

Summary of data for rsgA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to rsgA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7841

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2372

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4161

EcoGene

EcoGene:EG12479

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004337

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013626

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Arabidopsis thaliana

  • AT1G67440 (score: 1.000; bootstrap: 100%)
  • AT1G67460 (score: 0.506)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000004298 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000051137 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

YJEQ

From SHIGELLACYC

E. coli O157

YJEQ

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF03193 Protein of unknown function, DUF258

Panther (EcoliWiki Page)

PTHR11649:SF48

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

EcoCyc

EcoCyc:G7841

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12479

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004337

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2372

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013626

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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