rpsM:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rpsM

Mnemonic

Ribosomal protein, small

Synonyms

ECK3285, b3298, JW3260[1], JW3260

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.15 minutes 

MG1655: 3440493..3440137
<gbrowseImage> name=NC_000913:3440137..3440493 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3370376..3370020
<gbrowseImage> name=NC_012967:3370020..3370376 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3327294..3327650
<gbrowseImage> name=NC_012759:3327294..3327650 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4197945..4198301
<gbrowseImage> name=NC_007779:4197945..4198301 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3538238..3537882
<gbrowseImage> name=NC_010473:3537882..3538238 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3440140

Edman degradation

PMID:330375[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rpsM

Deletion

rpsM

Deletion

rpsMN105H

N105H

(in PW095; partially suppresses a rimM deletion)

Strain variation; seeded from UniProt:P0A7S9

rpsMN105K

N105K

(in PW097; partially suppresses a rimM deletion)

Strain variation; seeded from UniProt:P0A7S9

rpsM

Deletion

rpsM

Deletion

Ksgd rpsM MV34

Antibiotic Dependence

Kasugamycin Dependence

PMID:363701[3]

See figure 2 for portions of two-dimensional gel electropherogram of 70s ribosome protien.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3260

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGCCCGTATAGCAGGCATTAA

Primer 2:CCTTTCTTGATCGGTTTGCGCGG

E1H9

Kohara Phage

Genobase

PMID:3038334[5]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[6]

est. P1 cotransduction: 67% [7]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[6]

est. P1 cotransduction: 3% [7]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10912

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10912

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000903

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947791

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0905

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010809

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Lindemann, H & Wittmann-Liebold, B (1977) Primary structure of protein S13 from the small subunit of escherichia coli ribosomes. Hoppe-Seyler's Z. Physiol. Chem. 358 843-63 PubMed
  3. Dabbs, ER (1978) Kasugamycin-dependent mutants of Escherichia coli. J. Bacteriol. 136 994-1001 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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