rpsH:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rpsH

Mnemonic

Ribosomal protein, small

Synonyms

ECK3293, b3306, JW3268[1], JW3268

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.23 minutes, 74.23 minutes 

MG1655: 3444567..3444175
<gbrowseImage> name=NC_000913:3444175..3444567 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3374450..3374058
<gbrowseImage> name=NC_012967:3374058..3374450 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3331332..3331724
<gbrowseImage> name=NC_012759:3331332..3331724 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4193871..4194263
<gbrowseImage> name=NC_007779:4193871..4194263 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3542312..3541920
<gbrowseImage> name=NC_010473:3541920..3542312 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3444178

Edman degradation

PMID:365698[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rpsH::Tn5KAN-I-SceI (FB21105)

Insertion at nt 300 in Plus orientation

PMID:15262929[3]

E. coli Genome Project:FB21105

does not contain pKD46

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3268

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAGCATGCAAGATCCGATCGC

Primer 2:CCGGCTACGTAGCAGATAATTTC

E1H9

Kohara Phage

Genobase

PMID:3038334[5]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[6]

est. P1 cotransduction: 58% [7]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[6]

est. P1 cotransduction: 5% [7]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10907

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10907

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000898

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947802

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0900

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010829

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Allen, G & Wittmann-Liebold, B (1978) The amino acid sequence of the ribosomal protein S8 of Escherichia coli. Hoppe-Seyler's Z. Physiol. Chem. 359 1509-25 PubMed
  3. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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