rpoC:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
rpoC |
|---|---|
| Mnemonic |
RNA polymerase |
| Synonyms |
ECK3979, b3988, JW3951, tabD, tabB[1], tabB |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
90.17 minutes |
MG1655: 4183373..4187596 |
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NC_012967: 4164961..4169184 |
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NC_012759: 4073053..4077276 |
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W3110 |
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W3110: 3451331..3447108 |
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DH10B: 4283069..4287292 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
4183373 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
rpoC110(ts) |
Growth Phenotype |
temperature sensitive |
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rpoCE925A |
Glu 925 substituted with Ala |
None |
mutation in predicted ppGpp binding pocket doesn't affect response to ppGpp in vitro (Fig. 2) | ||||
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rpoC4(ts) |
Growth Phenotype |
temperature sensitive |
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rpoC397 |
16 bp deletion that produces a frameshift and truncation of the protein |
Growth Phenotype |
temperature-sensitive for growth at 43.5C |
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rpoC397 |
16 bp deletion that produces a frameshift and truncation of the protein |
Resistant to |
blocks growth of phage P2 at 33C |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3951 |
Plasmid clone |
Status:Clone OK Primer 1:gccAAAGATTTATTAAAGTTTCTGAAAGCGCAGAC Primer 2:ccCTCGTTATCAGAACCGCCCAGACCTGCGTTCAG | |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 30% [10] | ||
|
Linked marker |
est. P1 cotransduction: 69% [10] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Fujiki, H & Zurek, G (1975) The subunits of DNA-dependent RNA polymerase from E. coli: I. Amino acid analysis and primary structure of the N-terminal regions. FEBS Lett. 55 242-4 PubMed
- ↑ Ovchinnikov, YA et al. (1981) The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit. Eur. J. Biochem. 116 621-9 PubMed
- ↑ Ovchinnikov YuA, et al. (1982) The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit. Nucleic Acids Res. 10 4035-44 PubMed
- ↑ Vrentas, CE et al. (2008) Still looking for the magic spot: the crystallographically defined binding site for ppGpp on RNA polymerase is unlikely to be responsible for rRNA transcription regulation. J. Mol. Biol. 377 551-64 PubMed
- ↑ 6.0 6.1 Christie, GE et al. (1996) Escherichia coli rpoC397 encodes a temperature-sensitive C-terminal frameshift in the beta' subunit of RNA polymerase that blocks growth of bacteriophage P2. J. Bacteriol. 178 6991-3 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 CGSC: The Coli Genetics Stock Center
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
