PMID:6287430
| Citation |
Ovchinnikov YuA, , Monastyrskaya, GS, Gubanov, VV, Guryev, SO, Salomatina, IS, Shuvaeva, TM, Lipkin, VM and Sverdlov, ED (1982) The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit. Nucleic Acids Res. 10:4035-44 |
|---|---|
| Abstract |
The primary structure of the E. coli rpoC gene (5321 base pairs) coding the beta'-subunit of RNA polymerase as well as its adjacent segment have been determined. The structure analysis of the peptides obtained by cleavage of the protein with cyanogen bromide and trypsin has confirmed the amino acid sequence of the beta'-subunit deduced from the nucleotide sequence analysis. The beta'-subunit of E. coli RNA polymerase contains 1407 amino acid residues. Its translation is initiated by codon GUG and terminated by codon TAA. It has been detected that the sequence following the terminating codon is strikingly homologous to known sequences of rho-independent terminators. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Codon/genetics; DNA Restriction Enzymes; DNA-Directed RNA Polymerases/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Macromolecular Substances; Operon; Protein Biosynthesis |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.
