rpmC:Gene

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

<protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rpmC

Mnemonic

Ribosomal protein, large

Synonyms

ECK3299, b3312, JW3274[1], JW3274

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.29 minutes 

MG1655: 3446781..3446590
<gbrowseImage> name=NC_000913:3446590..3446781 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3376664..3376473
<gbrowseImage> name=NC_012967:3376473..3376664 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3333747..3333938
<gbrowseImage> name=NC_012759:3333747..3333938 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4191657..4191848
<gbrowseImage> name=NC_007779:4191657..4191848 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3544526..3544335
<gbrowseImage> name=NC_010473:3544335..3544526 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3446590

Edman degradation

PMID:1092361[2]
PMID:10739205[3]


<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Erm Dep rpmC

Protein L29 was removed

Growth Phenotype

Loss of erythromycin translation

PMID:387748[4]

It is unknown whether the loss of the protein causes erythromycin sensitivity. A known mutant is AM111(see table 1.). See figure 1

<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3274

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAAGCAAAAGAGCTGCGTGA

Primer 2:CCgGCACCCGCCTTCTCGTTCAG

E1H9

Kohara Phage

Genobase

PMID:3038334[6]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[7]

est. P1 cotransduction: 53% [8]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[7]

est. P1 cotransduction: 6% [8]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10887

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10887

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000878

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947807

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0880

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010842

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Bitar, KG (1975) The primary structure of the ribosomal protein L29 from Escherichia coli. Biochim. Biophys. Acta 386 99-106 PubMed
  3. Hoving, S et al. (2000) A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal. Chem. 72 1006-14 PubMed
  4. Dabbs, ER (1979) Selection for Escherichia coli mutants with proteins missing from the ribosome. J. Bacteriol. 140 734-7 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories