PMID:387748

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Citation

Dabbs, ER (1979) Selection for Escherichia coli mutants with proteins missing from the ribosome. J. Bacteriol. 140:734-7

Abstract

Antibiotic-independent revertants of an erythromycin-dependent strain of Escherichia coli were isolated by spontaneous selection. Their ribosomal proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. In contrast to most ribosomally targeted selections, the specific absence of a certain protein from the ribosome, rather than alterations in ribosomal proteins, was observed. Mutants were found with protein S20, L11, L15, L28, L29, or L30 missing.

Links

PubMed PMC216704

Keywords

Bacterial Proteins/analysis; Bacterial Proteins/genetics; Escherichia coli/analysis; Escherichia coli/genetics; Genes; Mutation; Ribosomal Proteins/analysis; Ribosomal Proteins/genetics

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