PMID:387748
Citation |
Dabbs, ER (1979) Selection for Escherichia coli mutants with proteins missing from the ribosome. J. Bacteriol. 140:734-7 |
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Abstract |
Antibiotic-independent revertants of an erythromycin-dependent strain of Escherichia coli were isolated by spontaneous selection. Their ribosomal proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. In contrast to most ribosomally targeted selections, the specific absence of a certain protein from the ribosome, rather than alterations in ribosomal proteins, was observed. Mutants were found with protein S20, L11, L15, L28, L29, or L30 missing. |
Links | |
Keywords |
Bacterial Proteins/analysis; Bacterial Proteins/genetics; Escherichia coli/analysis; Escherichia coli/genetics; Genes; Mutation; Ribosomal Proteins/analysis; Ribosomal Proteins/genetics |
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