rplB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rplB

Mnemonic

Ribosomal protein, large

Synonyms

ECK3304, b3317, JW3279[1], JW3279

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.33 minutes, 74.33 minutes 

MG1655: 3449386..3448565
<gbrowseImage> name=NC_000913:3448565..3449386 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3379269..3378448
<gbrowseImage> name=NC_012967:3378448..3379269 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3335722..3336543
<gbrowseImage> name=NC_012759:3335722..3336543 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4189052..4189873
<gbrowseImage> name=NC_007779:4189052..4189873 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3547131..3546310
<gbrowseImage> name=NC_010473:3546310..3547131 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3448568

Edman degradation

PMID:3892488[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rplBH230Q

H230Q

Loss of peptidyltransferase activity in reconstituted ribosomes. No change in rRNA binding or assembly into ribosomes

seeded from UniProt:P60422

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3279

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCGCAGTTGTTAAATGTAAACC

Primer 2:CCTTTGCTACGGCGACGTACGAT

E1H9

Kohara Phage

Genobase

PMID:3038334[4]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[5]

est. P1 cotransduction: 49% [6]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[5]

est. P1 cotransduction: 7% [6]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10865

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10865

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000856

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947820

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0858

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010854

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Zurawski, G & Zurawski, SM (1985) Structure of the Escherichia coli S10 ribosomal protein operon. Nucleic Acids Res. 13 4521-6 PubMed
  3. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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