rnt:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

rnt

Gene Synonym(s)

ECK1648, b1652, JW1644[1], JW1644

Product Desc.

Rnt[2][3];

Component of RNase T, degrades tRNA[3]

RNase T; RNA processing[4]

Product Synonyms(s)

ribonuclease T (RNase T)[1], B1652[2][1] , ECK1648, JW1644, b1652

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): rnt-lhr[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rnt

Mnemonic

RNase T

Synonyms

ECK1648, b1652, JW1644[1], JW1644

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

37.21 minutes 

MG1655: 1726371..1727018
<gbrowseImage> name=NC_000913:1726371..1727018 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1705541..1706188
<gbrowseImage> name=NC_012967:1705541..1706188 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1618430..1619077
<gbrowseImage> name=NC_012759:1618430..1619077 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1730061..1730708
<gbrowseImage> name=NC_007779:1730061..1730708 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1816942..1817589
<gbrowseImage> name=NC_010473:1816942..1817589 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δrnt (Keio:JW1644)

deletion

deletion

PMID:16738554

Shigen
CGSC9399[5]

Δrnt::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938

Δrnt::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938

Δrnt-730::kan

PMID:16738554

CGSC:102758


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1644

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCCGATAACGCTCAACTTAC

Primer 2:CCaACCTCTTCGGCGGCAGATAG

6F11

Kohara Phage

Genobase

PMID:3038334

4H6

Kohara Phage

Genobase

PMID:3038334

zdd-235::Tn10

Linked marker

CAG18461 = CGSC7378[5]

est. P1 cotransduction: % [6]
Synonyms:zdc-235::Tn10

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[5]

est. P1 cotransduction: 9% [6]
Synonyms:zdh-925::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11547

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11547

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001501

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946159

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1509

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005523

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Rnt

Synonyms

ribonuclease T (RNase T)[1], B1652[2][1] , ECK1648, JW1644, b1652

Product description

Rnt[2][3];

Component of RNase T, degrades tRNA[3]

RNase T; RNA processing[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012337

F

Seeded from EcoCyc (v14.0)

complete

GO:0004518

nuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0540

F

Seeded from EcoCyc (v14.0)

complete

GO:0004527

exonuclease activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006055

F

Seeded from EcoCyc (v14.0)

complete

GO:0004527

exonuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0269

F

Seeded from EcoCyc (v14.0)

complete

GO:0004540

ribonuclease activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005987

F

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006055

C

Seeded from EcoCyc (v14.0)

complete

GO:0006396

RNA processing

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005987

P

Seeded from EcoCyc (v14.0)

complete

GO:0008033

tRNA processing

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00157

P

Seeded from EcoCyc (v14.0)

complete

GO:0008033

tRNA processing

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0819

P

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0016896

exoribonuclease activity, producing 5'-phosphomonoesters

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00157

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of RNase T, degrades tRNA

could be indirect

Protein

aceF

PMID:15690043

Experiment(s):EBI-888598

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSDNAQLTGL CDRFRGFYPV VIDVETAGFN AKTDALLEIA AITLKMDEQG WLMPDTTLHF
HVEPFVGANL QPEALAFNGI DPNDPDRGAV SEYEALHEIF KVVRKGIKAS GCNRAIMVAH
NANFDHSFMM AAAERASLKR NPFHPFATFD TAALAGLALG QTVLSKACQT AGMDFDSTQA
HSALYDTERT AVLFCEIVNR WKRLGGWPLS AAEEV
Length

215

Mol. Wt

23.522 kDa

pI

5.2 (calculated)

Extinction coefficient

20,970 - 21,470 (calc based on 3 Y, 3 W, and 4 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

19..194

PF00929 Exonuclease

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rnt taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129610

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946159

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005523

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P30014

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11547

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11547

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946159

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001501

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1509

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

1089

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

204

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

345

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

rnt-lhr

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1726351..1726391 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1652 (EcoliWiki Page)

NCBI GEO profiles for rnt

microarray

GenExpDB:b1652 (EcoliWiki Page)

Summary of data for rnt from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (1726191..1726403) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ04; Well:E6[7]

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Notes

Accessions Related to rnt Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11547

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1509

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1652

EcoGene

EcoGene:EG11547

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001501

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005523

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

RNT

From SHIGELLACYC

E. coli O157

RNT

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00929 Exonuclease

Superfamily (EcoliWiki Page)

SUPERFAMILY:53098

EcoCyc

EcoCyc:EG11547

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11547

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001501

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1509

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005523

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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