rne:Gene
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
rne |
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Mnemonic |
RNase E |
Synonyms |
ECK1069, b1084, JW1071, ams, hmp1, rne_1, smbB[1], smbB |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
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MG1655 |
24.58 minutes |
MG1655: 1143590..1140405 |
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NC_012967: 1158936..1155751 |
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NC_012759: 1044330..1047515 |
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W3110 |
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W3110: 1145944..1142759 |
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DH10B: 1199035..1195850 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
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Coding Start (SO:0000323) |
1140405 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
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rne-3071(ts) |
temperature sensitive |
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rne3071 |
mRNA degredation |
RNase E deficient mutation causes accumulation of P1-t1 in strain due to lack of RNase E cleave of 3' terminal hairpin stricture, Figure 4. |
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rne3071 |
mRNA degredation |
This temperature sensitive mutation up regulates mutL-miaA which are co transcribed, and promoters Pmia and P1hfq by a factor of 3, 7 and 8 fold. Table 2. |
Strain:N3431 |
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
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JW1071 |
Plasmid clone |
Status:Clone OK Primer 1:gccAAAAGAATGTTAATCAACGCAACTCAG Primer 2:cctTCAACAGGTTGCGGACGCGCAGGAGCG | |
Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: % [10] | ||
Linked marker |
est. P1 cotransduction: 59% [10] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
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Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
Name | URL | Comments |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Chauhan, AK et al. (1991) Sequencing and expression of the rne gene of Escherichia coli. Nucleic Acids Res. 19 125-9 PubMed
- ↑ McDowall, KJ et al. (1993) The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus. J. Bacteriol. 175 4245-9 PubMed
- ↑ Babitzke, P & Kushner, SR (1991) The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 88 1-5 PubMed
- ↑ Hajnsdorf, E et al. (1994) Roles of RNase E, RNase II and PNPase in the degradation of the rpsO transcripts of Escherichia coli: stabilizing function of RNase II and evidence for efficient degradation in an ams pnp rnb mutant. EMBO J. 13 3368-77 PubMed
- ↑ Tsui, HC & Winkler, ME (1994) Transcriptional patterns of the mutL-miaA superoperon of Escherichia coli K-12 suggest a model for posttranscriptional regulation. Biochimie 76 1168-77 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 CGSC: The Coli Genetics Stock Center
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).