PMID:8320240
| Citation |
McDowall, KJ, Hernandez, RG, Lin-Chao, S and Cohen, SN (1993) The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus. J. Bacteriol. 175:4245-9 |
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| Abstract |
Two temperature-sensitive mutations, ams-1 and rne-3071, in the ams (altered mRNA stability) gene have been used extensively to investigate the processing and decay of RNA in Escherichia coli. We have sequenced these temperature-sensitive alleles and found that the mutations are separated by only 6 nucleotides and cause conservative amino acid substitutions next to a possible nucleotide-binding site within the N-terminal domain of the Ams protein. Computer analysis revealed that the region altered by the mutations has extensive sequence similarity to a predicted gene product from the mre (murein pathway cluster e) locus of E. coli, which has been implicated previously in determining bacterial cell shape. |
| Links | |
| Keywords |
Adenosine Triphosphate/metabolism; Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; Carrier Proteins/genetics; Cyclic AMP Receptor Protein; Endoribonucleases; Escherichia coli/genetics; Escherichia coli Proteins; Hot Temperature; Molecular Sequence Data; Mutation; RNA, Messenger/metabolism; Receptors, Cyclic AMP/genetics; Sequence Alignment/methods; Sequence Analysis, DNA; Sequence Homology, Amino Acid |
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