rna:Gene

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

<protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rna

Mnemonic

RNase A

Synonyms

ECK0604, b0611, JW0603, rnsA[1], rnsA

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

13.87 minutes 

MG1655: 644226..643420
<gbrowseImage> name=NC_000913:643420..644226 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 627054..626248
<gbrowseImage> name=NC_012967:626248..627054 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 546180..546986
<gbrowseImage> name=NC_012759:546180..546986 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 644226..643420
<gbrowseImage> name=NC_007779:643420..644226 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 583558..582752
<gbrowseImage> name=NC_010473:582752..583558 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 696818..696012
<gbrowseImage> name=NC_010473:696012..696818 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

643423

Edman degradation

PMID:2253883[2]


<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δrna (Keio:JW0603)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8718[4]

rna::Tn5KAN-2 (FB20214)

Insertion at nt 120 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB20214

does not contain pKD46

rna-1

CGSC:5434

rna-2

CGSC:5440

rna-19

CGSC:7374

rna-30::kan

PMID:1309522[6]

CGSC:40122

Δrna-749::kan

PMID:16738554[3]

CGSC:102742


<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0603

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAAAGCATTCTGGCGTAACGC

Primer 2:CCATAACCCGCTTTATCAATCAC

4A5

Kohara Phage

Genobase

PMID:3038334[8]

3G5

Kohara Phage

Genobase

PMID:3038334[8]

dsbG601::Tn10

Linked marker

CAG12149 = CGSC7346[4]

est. P1 cotransduction: 77% [9]
Synonyms:zbd-601::Tn10, zbe-601::Tn10

crcA280::Tn10

Linked marker

CAG12077 = CGSC7347[4]

est. P1 cotransduction: 69% [9]
Synonyms:zbe-280::Tn10

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10856

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10856

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000847

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:949065

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0849

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002107

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Meador, J 3rd & Kennell, D (1990) Cloning and sequencing the gene encoding Escherichia coli ribonuclease I: exact physical mapping using the genome library. Gene 95 1-7 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Srivastava, SK et al. (1992) Broad-specificity endoribonucleases and mRNA degradation in Escherichia coli. J. Bacteriol. 174 56-62 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories