rlmN:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rlmN

Mnemonic

rRNA large-subunit methylation

Synonyms

ECK2513, b2517, JW2501, yfgB[1], JW2501, yfgB

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

56.93 minutes 

MG1655: 2642305..2641151
<gbrowseImage> name=NC_000913:2641151..2642305 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2526956..2528110
<gbrowseImage> name=NC_012759:2526956..2528110 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2642939..2641785
<gbrowseImage> name=NC_007779:2641785..2642939 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔyfgB (Keio:JW2501)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9992[3]

yfgB::Tn5KAN-I-SceI (FB20783)

Insertion at nt 617 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20838

contains pKD46

yfgB::Tn5KAN-I-SceI (FB20783)

Insertion at nt 617 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20839

does not contain pKD46

ΔyfgB763::kan

PMID:16738554[2]

CGSC:104754


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2501

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCTCTGAACAATTAGTCACACC

Primer 2:CCGACCGCTTTAATGTCGATGGC

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[3]

est. P1 cotransduction: 12% [6]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[3]

est. P1 cotransduction: 44% [6]
Synonyms:zff-208::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12401

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12401

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002263

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946249

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2301

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008287

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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