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Expression|

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Standard Name	recX
Gene Synonym(s)	ECK2693, b2698, JW2668, oraA^[1], oraA
Product Desc.	inhibitor of RecA^[2]^[3] Blocks RecA filament extension; inhibitor of RecA ATPase, recombinase and coprotease activities; null mutant and over-expressors have reduced resistance to DNA damage; binding to RecA is antagonized by RecF^[4]
Product Synonyms(s)	regulatory protein for RecA^[1], B2698^[2]^[1], RecX^[2]^[1], OraA^[2]^[1] , ECK2693, JW2668, oraA, b2698
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): recAX^[2]
Regulation/Activity
Quick Links
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Notes

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RecX (negative) directly competes with DinI (positive) as modulators of RecA function. lexA regulon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	recX
Mnemonic	Recombination
Synonyms	ECK2693, b2698, JW2668, oraA^[1], oraA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	60.78 minutes	MG1655: 2820661..2820161 <gbrowseImage> name=NC_000913:2820161..2820661 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2717498..2716998 <gbrowseImage> name=NC_012967:2716998..2717498 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2705973..2706473 <gbrowseImage> name=NC_012759:2705973..2706473 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2821295..2820795 <gbrowseImage> name=NC_007779:2820795..2821295 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2913203..2912703 <gbrowseImage> name=NC_010473:2912703..2913203 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔrecX (Keio:JW2668)	deletion	deletion	PMID:16738554	Shigen
oraA::Tn5KAN-I-SceI (FB20882)	Insertion at nt 265 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20882	does not contain pKD46
oraA::Tn5KAN-I-SceI (FB20883)	Insertion at nt 265 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20883	contains pKD46
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2668	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACAGAATCAACATCCCGTCG Primer 2:CCGTCGGCAAAATTTCGCCAAAT
23B7	Kohara Phage	Genobase	PMID:3038334
pheA18::Tn10	Linked marker	CAG12158 = CGSC7421^[5]	est. P1 cotransduction: % ^[6]
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[5]	est. P1 cotransduction: 84% ^[6] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12080	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12080	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001986	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947172	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2005	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008872	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	RecX
Synonyms	regulatory protein for RecA^[1], B2698^[2]^[1], RecX^[2]^[1], OraA^[2]^[1] , ECK2693, JW2668, oraA, b2698
Product description	inhibitor of RecA^[2]^[3] Blocks RecA filament extension; inhibitor of RecA ATPase, recombinase and coprotease activities; null mutant and over-expressors have reduced resistance to DNA damage; binding to RecA is antagonized by RecF^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01114	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006281	DNA repair	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0234	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006282	regulation of DNA repair	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01114	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006282	regulation of DNA repair	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003783	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0227	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009432	SOS response	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0742	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	greB	PMID:16606699	Experiment(s):EBI-1143689
Protein	rimJ	PMID:16606699	Experiment(s):EBI-1143689
Protein	matC	PMID:16606699	Experiment(s):EBI-1143689
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTESTSRRPA YARLLDRAVR ILAVRDHSEQ ELRRKLAAPI MGKNGPEEID ATAEDYERVI AWCHEHGYLD DSRFVARFIA SRSRKGYGPA RIRQELNQKG ISREATEKAM RECDIDWCAL ARDQATRKYG EPLPTVFSEK VKIQRFLLYR GYLMEDIQEI WRNFAD
Length	166
Mol. Wt	19.424 kDa
pI	8.8 (calculated)
Extinction coefficient	26,930 - 27,305 (calc based on 7 Y, 3 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	46..166	PF02631 RecX family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=recX taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130605	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947172	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008872	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P33596	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12080	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12080	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947172	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001986	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2005	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	71	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	17	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	51^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
recAX
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2820641..2820681 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2698 (EcoliWiki Page)	NCBI GEO profiles for recX
microarray	GenExpDB:b2698 (EcoliWiki Page)	Summary of data for recX from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2820562..2820788) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ06; Well:D7^[7]
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Notes

Accessions Related to recX Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12080	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2005	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2698
EcoGene	EcoGene:EG12080	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001986	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008872	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT3G13226 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	ORAA	From SHIGELLACYC
E. coli O157	ORAA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02631 RecX family
EcoCyc	EcoCyc:EG12080	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12080	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001986	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2005	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008872	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

recX:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to recX Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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