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Standard Name	recO
Gene Synonym(s)	ECK2563, b2565, JW2549^[1], JW2549
Product Desc.	protein interacts with RecR and possibly RecF proteins^[2]^[3]; Component of RecFOR complex^[2]^[3] Conjugational recombination and repair; DNA-binding protein; RecA-like strand assimilation^[4]
Product Synonyms(s)	gap repair protein^[1], B2565^[2]^[1], RecO^[2]^[1] , ECK2563, JW2549, b2565
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): rnc-era-recO-pdxJ-acpS^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	recO
Mnemonic	Recombination
Synonyms	ECK2563, b2565, JW2549^[1], JW2549
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	58.19 minutes	MG1655: 2700491..2699763 <gbrowseImage> name=NC_000913:2699763..2700491 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2623961..2623233 <gbrowseImage> name=NC_012967:2623233..2623961 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2585568..2586296 <gbrowseImage> name=NC_012759:2585568..2586296 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2701125..2700397 <gbrowseImage> name=NC_007779:2700397..2701125 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2792256..2791528 <gbrowseImage> name=NC_010473:2791528..2792256 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔrecO (Keio:JW2549)	deletion	deletion			PMID:16738554	Shigen CGSC10028^[5]
ΔrecO737::kan					PMID:16738554	CGSC:102630
recO	deletion		Sensitivity to	increases sensitivity to Bicyclomycin	PMID:21357484		fig 2
recO1504::Tn5 (KD2240)			Resistant to	Mutation increases resistance to Thymineless Death (TLD). Figure 1.	PMID:2848620		Parent Strain: KD2197
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID:16769691 Status: Primer 1: Primer 2:
7G4	Kohara Phage	Genobase	PMID:3038334
zfh-208::Tn10	Linked marker	CAG18481 = CGSC7417^[5]	est. P1 cotransduction: 22% ^[6] Synonyms:zff-208::Tn10
nadB51::Tn10	Linked marker	CAG18480 = CGSC7419^[5]	est. P1 cotransduction: 72% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10832	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10832	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000823	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947038	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0825	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008438	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	RecO
Synonyms	gap repair protein^[1], B2565^[2]^[1], RecO^[2]^[1] , ECK2563, JW2549, b2565
Product description	protein interacts with RecR and possibly RecF proteins^[2]^[3]; Component of RecFOR complex^[2]^[3] Conjugational recombination and repair; DNA-binding protein; RecA-like strand assimilation^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006281	DNA repair	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00201	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006281	DNA repair	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003717	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006281	DNA repair	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0234	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00201	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003717	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0233	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0227	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of RecFOR complex	could be indirect
Protein	rpsE		PMID:16606699	Experiment(s):EBI-1143287
Protein	slt		PMID:16606699	Experiment(s):EBI-1143287
Protein	era		PMID:16606699	Experiment(s):EBI-1143287
Protein	aegA		PMID:16606699	Experiment(s):EBI-1143287
Protein	secD		PMID:16606699	Experiment(s):EBI-1143287
Protein	rplO		PMID:16606699	Experiment(s):EBI-1143287
Protein	rpsC		PMID:16606699	Experiment(s):EBI-1143287
Protein	rpsG		PMID:16606699	Experiment(s):EBI-1143287
Protein	rpsJ		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MEGWQRAFVL HSRPWSETSL MLDVFTEESG RVRLVAKGAR SKRSTLKGAL QPFTPLLLRF GGRGEVKTLR SAEAVSLALP LSGITLYSGL YINELLSRVL EYETRFSELF FDYLHCIQSL AGVTGTPEPA LRRFELALLG HLGYGVNFTH CAGSGEPVDD TMTYRYREEK GFIASVVIDN KTFTGRQLKA LNAREFPDAD TLRAAKRFTR MALKPYLGGK PLKSRELFRQ FMPKRTVKTH YE
Length	242
Mol. Wt	27.391 kDa
pI	10.2 (calculated)
Extinction coefficient	24,410 - 24,660 (calc based on 9 Y, 2 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	80..226	PF02565 Recombination protein O C terminal	PMID:19920124
Domain	1..78	PF11967 Recombination protein O N terminal	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=recO taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130490	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947038	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008438	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A7H3	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10832	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10832	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947038	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000823	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0825	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	0.286+/-0.14	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.039148352	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	85	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	34	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	29^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
rnc-era-recO-pdxJ-acpS
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2700471..2700511 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2565 (EcoliWiki Page)	NCBI GEO profiles for recO
microarray	GenExpDB:b2565 (EcoliWiki Page)	Summary of data for recO from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to recO Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10832	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0825	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2565
EcoGene	EcoGene:EG10832	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000823	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008438	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	RECO	From SHIGELLACYC
E. coli O157	RECO	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:50249
Superfamily (EcoliWiki Page)	SUPERFAMILY:57863
Pfam (EcoliWiki Page)	PF02565 Recombination protein O C terminal
Pfam (EcoliWiki Page)	PF11967 Recombination protein O N terminal
EcoCyc	EcoCyc:EG10832	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10832	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000823	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0825	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008438	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

recO:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to recO Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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