recO:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

recO

Mnemonic

Recombination

Synonyms

ECK2563, b2565, JW2549[1], JW2549

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

58.19 minutes 

MG1655: 2700491..2699763
<gbrowseImage> name=NC_000913:2699763..2700491 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2623961..2623233
<gbrowseImage> name=NC_012967:2623233..2623961 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2585568..2586296
<gbrowseImage> name=NC_012759:2585568..2586296 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2701125..2700397
<gbrowseImage> name=NC_007779:2700397..2701125 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2792256..2791528
<gbrowseImage> name=NC_010473:2791528..2792256 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrecO (Keio:JW2549)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10028[3]

ΔrecO737::kan

PMID:16738554[2]

CGSC:102630

recO

deletion

Sensitivity to

increases sensitivity to Bicyclomycin

PMID:21357484[4]

fig 2

recO1504::Tn5 (KD2240)

Resistant to

Mutation increases resistance to Thymineless Death (TLD). Figure 1.

PMID:2848620[5]

Parent Strain: KD2197

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[6]

Status:

Primer 1:

Primer 2:

7G4

Kohara Phage

Genobase

PMID:3038334[7]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[3]

est. P1 cotransduction: 22% [8]
Synonyms:zff-208::Tn10

nadB51::Tn10

Linked marker

CAG18480 = CGSC7419[3]

est. P1 cotransduction: 72% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10832

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10832

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000823

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947038

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0825

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008438

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
  5. Nakayama, K et al. (1988) Thymineless death in Escherichia coli mutants deficient in the RecF recombination pathway. Can. J. Microbiol. 34 905-7 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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