rcsB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rcsB

Mnemonic

Regulator of capsule synthesis

Synonyms

ECK2210, b2217, JW2205[1], JW2205, viaA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

49.88 minutes 

MG1655: 2314199..2314849
<gbrowseImage> name=NC_000913:2314199..2314849 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2200004..2200654
<gbrowseImage> name=NC_012759:2200004..2200654 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2319511..2320161
<gbrowseImage> name=NC_007779:2319511..2320161 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2405187..2405837
<gbrowseImage> name=NC_010473:2405187..2405837 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrcsB (Keio:JW2205)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9783[3]

rcsB::Tn5KAN-2 (FB20713)

Insertion at nt 502 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20713

contains pKD46

ΔrcsB1320

CGSC:77155

ΔrcsB770::kan

PMID:16738554[2]

CGSC:102612

RcsB

A deletion 25 base pairs upstream of the ftsA1p -35 box

Gene Expression

A deletion within 25 base pairs upstream of the -35 box ceased RcsB activity, especially if it was within 15 base pairs or less of the box.

PMID:10564486[5]

See figure 1

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2205

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAACAATATGAACGTAATTAT

Primer 2:CCGTCTTTATCTGCCGGACTTAA

22A6

Kohara Phage

Genobase

PMID:3038334[7]

5A1

Kohara Phage

Genobase

PMID:3038334[7]

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[3]

est. P1 cotransduction: 64% [8]
Synonyms:zeg-722, zei-722::Tn10

atoS298::Tn10

Linked marker

CAG12177 gyrA+ = CGSC7525[3]

est. P1 cotransduction: 83% [8]
Synonyms:zeh-298::Tn10, zej-298::Tn10 nnnThe original CAG12177 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10821

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10821

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000812

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947441

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0814

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007333

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Carballès, F et al. (1999) Regulation of Escherichia coli cell division genes ftsA and ftsZ by the two-component system rcsC-rcsB. Mol. Microbiol. 34 442-50 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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