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Expression|

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Standard Name	ratB
Gene Synonym(s)	ECK2614, b2618, JW2599^[1], yfjF, JW2599
Product Desc.	"RatB is predicted to be the antitoxin for the RatA toxin, but no antitoxin activity was observed (Zhang & Inouye 2011)."^[2] Member of UPF0125 family.^[2]
Product Synonyms(s)	predicted protein^[1], B2618^[3]^[1], YfjF^[3]^[1] , RatB, ECK2614, JW2599, b2618
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yfjF^[3]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ratB
Mnemonic
Synonyms	ECK2614, b2618, JW2599^[1], yfjF, JW2599
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	59.32 minutes	MG1655: 2752320..2752030 <gbrowseImage> name=NC_000913:2752030..2752320 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2675593..2675303 <gbrowseImage> name=NC_012967:2675303..2675593 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2637842..2638132 <gbrowseImage> name=NC_012759:2637842..2638132 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2752954..2752664 <gbrowseImage> name=NC_007779:2752664..2752954 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2844085..2843795 <gbrowseImage> name=NC_010473:2843795..2844085 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔyfjF (Keio:JW2599)	deletion	deletion	PMID:16738554	Shigen CGSC10056^[4]
ΔyfjF774::kan			PMID:16738554	CGSC:104820
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2599	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCCAGGTAAAATTGCCGTTGA Primer 2:CCTTTATTCGCTGATTTTTCTGC
pheA18::Tn10	Linked marker	CAG12158 = CGSC7421^[4]	est. P1 cotransduction: 60% ^[5]
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[4]	est. P1 cotransduction: 1% ^[5] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7357	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13192	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003925	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945078	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2984	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008616	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	RatB
Synonyms	predicted protein^[1], B2618^[3]^[1], YfjF^[3]^[1] , RatB, ECK2614, JW2599, b2618
Product description	"RatB is predicted to be the antitoxin for the RatA toxin, but no antitoxin activity was observed (Zhang & Inouye 2011)."^[2] Member of UPF0125 family.^[2]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rplB	PMID:16606699	Experiment(s):EBI-1143500
Protein	rpsI	PMID:16606699	Experiment(s):EBI-1143500
Protein	pepD	PMID:16606699	Experiment(s):EBI-1143500
Protein	rpsG	PMID:16606699	Experiment(s):EBI-1143500
Protein	yhgF	PMID:16606699	Experiment(s):EBI-1143500
Protein	rpsD	PMID:16606699	Experiment(s):EBI-1143500
Protein	rpoB	PMID:16606699	Experiment(s):EBI-1143500
Protein	slyD	PMID:16606699	Experiment(s):EBI-1143500
Protein	putA	PMID:16606699	Experiment(s):EBI-1143500
Protein	rpsC	PMID:16606699	Experiment(s):EBI-1143500
Protein	rplD	PMID:16606699	Experiment(s):EBI-1143500
Protein	hsdR	PMID:16606699	Experiment(s):EBI-1143500
Protein	dnaK	PMID:16606699	Experiment(s):EBI-1143500
Protein	rhsD	PMID:16606699	Experiment(s):EBI-1143500
Protein	rplF	PMID:16606699	Experiment(s):EBI-1143500
Protein	nfnB	PMID:16606699	Experiment(s):EBI-1143500
Protein	rpoC	PMID:16606699	Experiment(s):EBI-1143500
Protein	lacZ	PMID:16606699	Experiment(s):EBI-1143500
Protein	rhsC	PMID:16606699	Experiment(s):EBI-1143500
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRKRFTVPGK IAVEVAYALP EKQYLQRVTL QEGATVEEAI RASGLLELRT DIDLTKNKVG IYSRPAKLSD SVHDGDRVEI YRPLIADPKE LRRQRAEKSA NK
Length	102
Mol. Wt	11.577 kDa
pI	10.0 (calculated)
Extinction coefficient	5,960 (calc based on 4 Y, 0 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	5..88	PF03658 Uncharacterised protein family (UPF0125)		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yfjF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111469	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945078	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008616	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P52119	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7357	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13192	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945078	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003925	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2984	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	1937	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	273	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	369	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yfjF
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2752300..2752340 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2618 (EcoliWiki Page)	NCBI GEO profiles for yfjF
microarray	GenExpDB:b2618 (EcoliWiki Page)	Summary of data for yfjF from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to yfjF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7357	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2984	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2618
EcoGene	EcoGene:EG13192	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003925	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008616	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	S2855	From SHIGELLACYC
E. coli O157	Z3911	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03658 Uncharacterised protein family (UPF0125)
EcoCyc	EcoCyc:G7357	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13192	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003925	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2984	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008616	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

ratB:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yfjF Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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