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Expression|

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Standard Name	puuA
Gene Synonym(s)	ECK1292, b1297, JW5201, ycjK^[1], ycjK
Product Desc.	γ-glutamylputrescine synthetase^[2]^[3] gamma-Glutamylputrescine synthase, ATP-dependent; putrescine utilization pathway; glutamate-putrescine ligase^[4]
Product Synonyms(s)	gamma-Glu-putrescine synthase^[1], B1297^[2]^[1], YcjK^[2]^[1], PuuA^[2]^[1] , ECK1292, JW5201, ycjK, b1297
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): puuA^[2]
Regulation/Activity
Quick Links
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Notes

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puuA is transiently induced after a cold shock. AFMB Structural Genomics target No. 77 (http://afmb.cnrs-mrs.fr/article171.html).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	puuA
Mnemonic	PUtrescine Utilization
Synonyms	ECK1292, b1297, JW5201, ycjK^[1], ycjK
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	29.26 minutes	MG1655: 1358932..1357514 <gbrowseImage> name=NC_000913:1357514..1358932 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1357858..1359276 <gbrowseImage> name=NC_012967:1357858..1359276 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1248363..1249781 <gbrowseImage> name=NC_012759:1248363..1249781 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1362622..1361204 <gbrowseImage> name=NC_007779:1361204..1362622 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1448328..1446910 <gbrowseImage> name=NC_010473:1446910..1448328 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔpuuA (Keio:JW5201)	deletion	deletion	PMID:16738554	Shigen CGSC11235^[5]
ΔpuuA733::kan			PMID:16738554	CGSC:102554
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5201	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGAAACCAATATCGTTGAAGT Primer 2:CCtGCGTTTTTCAACATCCACTC
E14F6	Kohara Phage	Genobase	PMID:3038334
1C2	Kohara Phage	Genobase	PMID:3038334
trpC83::Tn10	Linked marker	CAG18455 = CGSC7371^[5]	est. P1 cotransduction: 19% ^[6] Synonyms:trpB83::Tn10
zcj-233::Tn10	Linked marker	CAG12028 = CGSC7373^[5]	est. P1 cotransduction: 68% ^[6] Synonyms:zci-233::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6644	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13908	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003239	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946202	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3667	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004365	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PuuA
Synonyms	gamma-Glu-putrescine synthase^[1], B1297^[2]^[1], YcjK^[2]^[1], PuuA^[2]^[1] , ECK1292, JW5201, ycjK, b1297
Product description	γ-glutamylputrescine synthetase^[2]^[3] gamma-Glutamylputrescine synthase, ATP-dependent; putrescine utilization pathway; glutamate-putrescine ligase^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004356	glutamate-ammonia ligase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008146	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004356	glutamate-ammonia ligase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008147	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006542	glutamine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008147	P	Seeded from EcoCyc (v14.0)	complete
	GO:0034024	glutamate-putrescine ligase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:6.3.1.11	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MHTDSNGGHY ILRFDNERKT NQRRRIMETN IVEVENFVQQ SEERRGSAFT QEVKRYLERY PNTQYVDVLL TDLNGCFRGK RIPVSSLKKL EKGCYFPASV FAMDILGNVV EEAGLGQEMG EPDRTCVPVL GSLTPSAADP EFIGQMLLTM VDEDGAPFDV EPRNVLNRLW QQLRQRGLFP VVAVELEFYL LDRQRDAEGY LQPPCAPGTD DRNTQSQVYS VDNLNHFADV LNDIDELAQL QLIPADGAVA EASPGQFEIN LYHTDNVLEA CDDALALKRL VRLMAEKHKM HATFMAKPYE EHAGSGMHIH ISMQNNRGEN VLSDAEGEDS PLLKKMLAGM IDLMPSSMAL LAPNVNSYRR FQPGMYVPTQ ASWGHNNRTV ALRIPCGDRH NHRVEYRVAG ADANPYLVMA AIFAGILHGL DNELPLQEEV EGNGLEQEGL PFPIRQSDAL GEFIENDHLR RYLGERFCHV YHACKNDELL QFERLITETE IEWMLKNA
Length	498
Mol. Wt	56.374 kDa
pI	4.9 (calculated)
Extinction coefficient	40,340 - 41,340 (calc based on 16 Y, 3 W, and 8 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	133..390	PF00120 Glutamine synthetase, catalytic domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=puuA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111244	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946202	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004365	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P78061	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6644	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13908	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946202	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003239	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3667	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	109	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	822	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	152	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
puuA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1358912..1358952 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1297 (EcoliWiki Page)	NCBI GEO profiles for puuA
microarray	GenExpDB:b1297 (EcoliWiki Page)	Summary of data for puuA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1358940..1359208) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ02; Well:G12^[7]
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Notes

Accessions Related to puuA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6644	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3667	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1297
EcoGene	EcoGene:EG13908	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003239	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004365	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Bos taurus	ENSBTAP00000035459 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000003622 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0231552 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000026189 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000274922 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000007463 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000023318 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:2672844 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000031292 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000016316 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000156173 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00004895001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000049235 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Shigella flexneri	S1384	From SHIGELLACYC
E. coli O157	Z2491	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00120 Glutamine synthetase, catalytic domain
Panther (EcoliWiki Page)	PTHR20852:SF38
Superfamily (EcoliWiki Page)	SUPERFAMILY:54368
Superfamily (EcoliWiki Page)	SUPERFAMILY:55931
EcoCyc	EcoCyc:G6644	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13908	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003239	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3667	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004365	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

puuA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to puuA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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