puuA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PuuA

Synonyms

gamma-Glu-putrescine synthase[1], B1297[2][1], YcjK[2][1], PuuA[2][1] , ECK1292, JW5201, ycjK, b1297

Product description

γ-glutamylputrescine synthetase[2][3]

gamma-Glutamylputrescine synthase, ATP-dependent; putrescine utilization pathway; glutamate-putrescine ligase[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0004356

glutamate-ammonia ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008146

F

Seeded from EcoCyc (v14.0)

complete

GO:0004356

glutamate-ammonia ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008147

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0006542

glutamine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008147

P

Seeded from EcoCyc (v14.0)

complete

GO:0034024

glutamate-putrescine ligase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:6.3.1.11

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MHTDSNGGHY ILRFDNERKT NQRRRIMETN IVEVENFVQQ SEERRGSAFT QEVKRYLERY
PNTQYVDVLL TDLNGCFRGK RIPVSSLKKL EKGCYFPASV FAMDILGNVV EEAGLGQEMG
EPDRTCVPVL GSLTPSAADP EFIGQMLLTM VDEDGAPFDV EPRNVLNRLW QQLRQRGLFP
VVAVELEFYL LDRQRDAEGY LQPPCAPGTD DRNTQSQVYS VDNLNHFADV LNDIDELAQL
QLIPADGAVA EASPGQFEIN LYHTDNVLEA CDDALALKRL VRLMAEKHKM HATFMAKPYE
EHAGSGMHIH ISMQNNRGEN VLSDAEGEDS PLLKKMLAGM IDLMPSSMAL LAPNVNSYRR
FQPGMYVPTQ ASWGHNNRTV ALRIPCGDRH NHRVEYRVAG ADANPYLVMA AIFAGILHGL
DNELPLQEEV EGNGLEQEGL PFPIRQSDAL GEFIENDHLR RYLGERFCHV YHACKNDELL
QFERLITETE IEWMLKNA
Length

498

Mol. Wt

56.374 kDa

pI

4.9 (calculated)

Extinction coefficient

40,340 - 41,340 (calc based on 16 Y, 3 W, and 8 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

133..390

PF00120 Glutamine synthetase, catalytic domain

PMID:19920124[5]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=puuA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111244

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946202

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004365

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P78061

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6644

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13908

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946202

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003239

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3667

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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