purL:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

purL

Gene Synonym(s)

ECK2555, b2557, JW2541, purI[1], purG, purI

Product Desc.

Phosphoribosylformylglycinamide synthase; contains a synthase domain (N) and a glutamine amidotransferase domain (C)[2]

Product Synonyms(s)

phosphoribosylformyl-glycineamide synthetase[1], B2557[3][1], PurI[3][1], PurL[3][1] , ECK2555, JW2541, purG, purI, b2557

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): purL[3], OP00079

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

purL

Mnemonic

Purine

Synonyms

ECK2555, b2557, JW2541, purI[1], purG, purI

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

57.97 minutes 

MG1655: 2693565..2689678
<gbrowseImage> name=NC_000913:2689678..2693565 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2616260..2612373
<gbrowseImage> name=NC_012967:2612373..2616260 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2575483..2579370
<gbrowseImage> name=NC_012759:2575483..2579370 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2694199..2690312
<gbrowseImage> name=NC_007779:2690312..2694199 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2785330..2781443
<gbrowseImage> name=NC_010473:2781443..2785330 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2689678

Edman degradation

PMID:2659070


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔpurL (Keio:JW2541)

deletion

deletion

PMID:16738554

Shigen
CGSC10023[4]

purL66

CGSC:8952

ΔpurL731::kan

PMID:16738554

CGSC:102538


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2541

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCATGGAAATTCTGCGTGGTTC

Primer 2:CCgCCCAACTGCTTACGCGCATT

6H2

Kohara Phage

Genobase

PMID:3038334

7G4

Kohara Phage

Genobase

PMID:3038334

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[4]

est. P1 cotransduction: 36% [5]
Synonyms:zff-208::Tn10

nadB51::Tn10

Linked marker

CAG18480 = CGSC7419[4]

est. P1 cotransduction: 48% [5]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10797

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10797

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000788

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947032

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0790

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008412

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PurL

Synonyms

phosphoribosylformyl-glycineamide synthetase[1], B2557[3][1], PurI[3][1], PurL[3][1] , ECK2555, JW2541, purG, purI, b2557

Product description

Phosphoribosylformylglycinamide synthase; contains a synthase domain (N) and a glutamine amidotransferase domain (C)[2]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000728

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016188

F

Seeded from EcoCyc (v14.0)

complete

GO:0004642

phosphoribosylformylglycinamidine synthase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00419

F

Seeded from EcoCyc (v14.0)

complete

GO:0004642

phosphoribosylformylglycinamidine synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010073

F

Seeded from EcoCyc (v14.0)

complete

GO:0004642

phosphoribosylformylglycinamidine synthase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:6.3.5.3

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00419

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00419

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006164

purine nucleotide biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00419

P

Seeded from EcoCyc (v14.0)

complete

GO:0006164

purine nucleotide biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0658

P

Seeded from EcoCyc (v14.0)

complete

GO:0006189

'de novo' IMP biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010073

P

Seeded from EcoCyc (v14.0)

complete

GO:0006541

glutamine metabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0315

P

Seeded from EcoCyc (v14.0)

complete

GO:0016874

ligase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0436

F

Seeded from EcoCyc (v14.0)

complete

GO:0004642

phosphoribosylformylglycinamidine synthase activity

PMID:6343356

IMP: Inferred from Mutant Phenotype

F

complete

GO:0006189

'de novo' IMP biosynthetic process

PMID:6343356

IC: Inferred by Curator

GO:0004642


P

FGAR to FGAM activity

complete

GO:0006541

glutamine metabolic process

PMID:2659070

IDA: Inferred from Direct Assay

P

complete

GO:0042623

ATPase activity, coupled

PMID:2659070

IDA: Inferred from Direct Assay

F

complete

GO:0005737

cytoplasm

PMID:2659070

IDA: Inferred from Direct Assay

C

complete

GO:0005524

ATP binding

PMID:2659070

IDA: Inferred from Direct Assay

F

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

dnaJ

PMID:16606699

Experiment(s):EBI-1143250

Protein

lon

PMID:16606699

Experiment(s):EBI-1143250

Protein

narG

PMID:16606699

Experiment(s):EBI-1143250

Protein

narZ

PMID:16606699

Experiment(s):EBI-1143250

Protein

prs

PMID:16606699

Experiment(s):EBI-1143250

Protein

hdeB

PMID:19402753

LCMS(ID Probability):99.6

Protein

hns

PMID:19402753

LCMS(ID Probability):99.6

Protein

nadC

PMID:19402753

LCMS(ID Probability):99.6

Protein

purA

PMID:19402753

LCMS(ID Probability):99.6

Protein

ybhR

PMID:19402753

LCMS(ID Probability):99.4

Protein

yigB

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MMEILRGSPA LSAFRINKLL ARFQAARLPV HNIYAEYVHF ADLNAPLNDD EHAQLERLLK
YGPALASHAP QGKLLLVTPR PGTISPWSSK ATDIAHNCGL QQVNRLERGV AYYIEAGTLT
NEQWQQVTAE LHDRMMETVF FALDDAEQLF AHHQPTPVTS VDLLGQGRQA LIDANLRLGL
ALAEDEIDYL QDAFTKLGRN PNDIELYMFA QANSEHCRHK IFNADWVIDG EQQPKSLFKM
IKNTFETTPD HVLSAYKDNA AVMEGSEVGR YFADHETGRY DFHQEPAHIL MKVETHNHPT
AISPWPGAAT GSGGEIRDEG ATGRGAKPKA GLVGFSVSNL RIPGFEQPWE EDFGKPERIV
TALDIMTEGP LGGAAFNNEF GRPALNGYFR TYEEKVNSHN GEELRGYHKP IMLAGGIGNI
RADHVQKGEI NVGAKLVVLG GPAMNIGLGG GAASSMASGQ SDADLDFASV QRDNPEMERR
CQEVIDRCWQ LGDANPILFI HDVGAGGLSN AMPELVSDGG RGGKFELREI LSDEPGMSPL
EIWCNESQER YVLAVAADQL PLFDELCKRE RAPYAVIGEA TEELHLSLHD RHFDNQPIDL
PLDVLLGKTP KMTRDVQTLK AKGDALAREG ITIADAVKRV LHLPTVAEKT FLVTIGDRSV
TGMVARDQMV GPWQVPVANC AVTTASLDSY YGEAMAIGER APVALLDFAA SARLAVGEAL
TNIAATQIGD IKRIKLSANW MAAAGHPGED AGLYEAVKAV GEELCPALGL TIPVGKDSMS
MKTRWQEGNE EREMTSPLSL VISAFARVED VRHTITPQLS TEDNALLLID LGKGNNALGA
TALAQVYRQL GDKPADVRDV AQLKGFYDAI QALVAQRKLL AYHDRSDGGL LVTLAEMAFA
GHCGIDADIA TLGDDRLAAL FNEELGAVIQ VRAADREAVE SVLAQHGLAD CVHYVGQAVS
GDRFVITANG QTVFSESRTT LRVWWAETTW QMQRLRDNPE CADQEHQAKS NDADPGLNVK
LSFDINEDVA APYIATGARP KVAVLREQGV NSHVEMAAAF HRAGFDAIDV HMSDLLTGRT
GLEDFHALVA CGGFSYGDVL GAGEGWAKSI LFNDRVRDEF ATFFHRPQTL ALGVCNGCQM
MSNLRELIPG SELWPRFVRN TSDRFEARFS LVEVTQSPSL LLQGMVGSQM PIAVSHGEGR
VEVRDAAHLA ALESKGLVAL RYVDNFGKVT ETYPANPNGS PNGITAVTTE SGRVTIMMPH
PERVFRTVSN SWHPENWGED GPWMRIFRNA RKQLG
Length

1,295

Mol. Wt

141.403 kDa

pI

5.2 (calculated)

Extinction coefficient

137,740 - 139,490 (calc based on 26 Y, 18 W, and 14 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

257..389

PF00586 AIR synthase related protein, N-terminal domain

PMID:19920124

Domain

431..591

PF02769 AIR synthase related protein, C-terminal domain

PMID:19920124

Domain

821..969

PF02769 AIR synthase related protein, C-terminal domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=purL taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:49176239

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947032

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008412

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P15254

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10797

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10797

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947032

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000788

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0790

Escherichia coli str. K-12 substr. MG1655

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Notes

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

6.94E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

39.44+/-0.329

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.112940571

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

1132

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1417

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

339

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

purL

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2693545..2693585 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2557 (EcoliWiki Page)

NCBI GEO profiles for purL

microarray

GenExpDB:b2557 (EcoliWiki Page)

Summary of data for purL from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to purL Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10797

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0790

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2557

EcoGene

EcoGene:EG10797

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000788

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008412

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

PURL

From SHIGELLACYC

E. coli O157

PURL

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00586 AIR synthase related protein, N-terminal domain

Pfam (EcoliWiki Page)

PF02769 AIR synthase related protein, C-terminal domain

Pfam (EcoliWiki Page)

PF02769 AIR synthase related protein, C-terminal domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:52317

Panther (EcoliWiki Page)

PTHR10099:SF0

Superfamily (EcoliWiki Page)

SUPERFAMILY:55326

Superfamily (EcoliWiki Page)

SUPERFAMILY:55326

Superfamily (EcoliWiki Page)

SUPERFAMILY:56042

Superfamily (EcoliWiki Page)

SUPERFAMILY:56042

Superfamily (EcoliWiki Page)

SUPERFAMILY:82697

Superfamily (EcoliWiki Page)

SUPERFAMILY:109736

EcoCyc

EcoCyc:EG10797

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10797

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000788

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0790

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008412

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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