ptsA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

ptsA

Gene Synonym(s)

ECK3939, b3947, JW5555, pt1A, frwA, yijH[1], yijH

Product Desc.

PEP-protein phosphotransferase system enzyme I[2][3]

Putative phosphotransferase system enzyme I; also has N-terminal Hpr-like domain and C-terminal Enzyme IIA domain[4]

Product Synonyms(s)

fused predicted PTS enzymes: Hpr component[1], enzyme I component[1], enzyme IIA component[1], B3947[2][1], FrwA[2][1], YijH[2][1], PtsA[2][1] , ECK3939, frwA, JW5555, yijH, b3947

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): gldA[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ptsA

Mnemonic

Phosphotransferase system

Synonyms

ECK3939, b3947, JW5555, pt1A, frwA, yijH[1], yijH

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

89.18 minutes, 89.18 minutes 

MG1655: 4140244..4137743
<gbrowseImage> name=NC_000913:4137743..4140244 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4122328..4119827
<gbrowseImage> name=NC_012967:4119827..4122328 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4027412..4029913
<gbrowseImage> name=NC_012759:4027412..4029913 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3494460..3496961
<gbrowseImage> name=NC_007779:3494460..3496961 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4239941..4237440
<gbrowseImage> name=NC_010473:4237440..4239941 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔptsA (Keio:JW5555)

deletion

deletion

PMID:16738554

Shigen
CGSC11461[5]

ΔptsA734::kan

PMID:16738554

CGSC:102516


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5555

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCCCTGATTGTGGAATTTAT

Primer 2:CCaAGTTCCAGTTCATGTTGCAG

8H10

Kohara Phage

Genobase

PMID:3038334

6D8

Kohara Phage

Genobase

PMID:3038334

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[5]

est. P1 cotransduction: 1% [6]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[5]

est. P1 cotransduction: 83% [6]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11906

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11906

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001835

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948437

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1851

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012920

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PtsA

Synonyms

fused predicted PTS enzymes: Hpr component[1], enzyme I component[1], enzyme IIA component[1], B3947[2][1], FrwA[2][1], YijH[2][1], PtsA[2][1] , ECK3939, frwA, JW5555, yijH, b3947

Product description

PEP-protein phosphotransferase system enzyme I[2][3]

Putative phosphotransferase system enzyme I; also has N-terminal Hpr-like domain and C-terminal Enzyme IIA domain[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015813

F

Seeded from EcoCyc (v14.0)

complete

GO:0005215

transporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016152

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000032

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001020

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002178

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005698

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008731

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008731

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001020

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002178

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016152

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0008643

carbohydrate transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0762

P

Seeded from EcoCyc (v14.0)

complete

GO:0008965

phosphoenolpyruvate-protein phosphotransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008731

F

Seeded from EcoCyc (v14.0)

complete

GO:0008965

phosphoenolpyruvate-protein phosphotransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.3.9

F

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000032

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001020

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002178

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005698

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008731

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0598

P

Seeded from EcoCyc (v14.0)

complete

GO:0016301

kinase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc (v14.0)

complete

GO:0016310

phosphorylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000121

P

Seeded from EcoCyc (v14.0)

complete

GO:0016310

phosphorylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008279

P

Seeded from EcoCyc (v14.0)

complete

GO:0016310

phosphorylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018274

P

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0016772

transferase activity, transferring phosphorus-containing groups

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000121

F

Seeded from EcoCyc (v14.0)

complete

GO:0016772

transferase activity, transferring phosphorus-containing groups

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006318

F

Seeded from EcoCyc (v14.0)

complete

GO:0016772

transferase activity, transferring phosphorus-containing groups

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008279

F

Seeded from EcoCyc (v14.0)

complete

GO:0016772

transferase activity, transferring phosphorus-containing groups

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018274

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MALIVEFICE LPNGVHARPA SHVETLCNTF SSQIEWHNLR TDRKGNAKSA LALIGTDTLA
GDNCQLLISG ADEQEAHQRL SQWLRDEFPH CDAPLAEVKS DELEPLPVSL TNLNPQIIRA
RTVCSGSAGG ILTPISSLDL NALGNLPAAK GVDAEQSALE NGLTLVLKNI EFRLLDSDGA
TSAILEAHRS LAGDTSLREH LLAGVSAGLS CAEAIVASAN HFCEEFSRSS SSYLQERALD
VRDVCFQLLQ QIYGEQRFPA PGKLTQPAIC MADELTPSQF LELDKNHLKG LLLKSGGTTS
HTVILARSFN IPTLVGVDID ALTPWQQQTI YIDGNAGAIV VEPGEAVARY YQQEARVQDA
LREQQRVWLT QQARTADGIR IEIAANIAHS VEAQAAFGNG AEGVGLFRTE MLYMDRTSAP
GESELYNIFC QALESANGRS IIVRTMDIGG DKPVDYLNIP AEANPFLGYR AVRIYEEYAS
LFTTQLRSIL RASAHGSLKI MIPMISSMEE ILWVKEKLAE AKQQLRNEHI PFDEKIQLGI
MLEVPSVMFI IDQCCEEIDF FSIGSNDLTQ YLLAVDRDNA KVTRHYNSLN PAFLRALDYA
VQAVHRQGKW IGLCGELGAK GSVLPLLVGL GLDELSMSAP SIPAAKARMA QLDSRECRKL
LNQAMACRTS LEVEHLLAQF RMTQQDAPLV TAECITLESD WRSKEEVLKG MTDNLLLAGR
CRYPRKLEAD LWAREAVFST GLGFSFAIPH SKSEHIEQST ISVARLQAPV RWGDDEAQFI
IMLTLNKHAA GDQHMRIFSR LARRIMHEEF RNALVNAASA DAIASLLQHE LEL
Length

833

Mol. Wt

91.775 kDa

pI

4.4 (calculated)

Extinction coefficient

71,850 - 73,975 (calc based on 15 Y, 9 W, and 17 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Modification Site

669

phosphorylation site at T669

probability less than 75%

PMID:17938405

Modification Site

670

phosphorylation site at S670

probability less than 75%

PMID:17938405

Domain

259..337

PF00391 PEP-utilising enzyme, mobile domain

PMID:19920124

Domain

362..654

PF02896 PEP-utilising enzyme, TIM barrel domain

PMID:19920124

Domain

2..87

PF00381 PTS HPr component phosphorylation site

PMID:19920124

Domain

688..830

PF00359 Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2

PMID:19920124

Domain

118..238

PF05524 PEP-utilising enzyme, N-terminal

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ptsA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:49176446

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948437

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012920

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P32670

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11906

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11906

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948437

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001835

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1851

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

4a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

2a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

gldA

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4140224..4140264 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3947 (EcoliWiki Page)

NCBI GEO profiles for ptsA

microarray

GenExpDB:b3947 (EcoliWiki Page)

Summary of data for ptsA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (4139354..4139469) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ16; Well:D8[7]

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Notes

Accessions Related to ptsA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11906

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1851

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3947

EcoGene

EcoGene:EG11906

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001835

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012920

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments
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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00391 PEP-utilising enzyme, mobile domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:47831

Superfamily (EcoliWiki Page)

SUPERFAMILY:51621

Superfamily (EcoliWiki Page)

SUPERFAMILY:52009

Superfamily (EcoliWiki Page)

SUPERFAMILY:55594

Superfamily (EcoliWiki Page)

SUPERFAMILY:55804

Pfam (EcoliWiki Page)

PF02896 PEP-utilising enzyme, TIM barrel domain

Pfam (EcoliWiki Page)

PF00381 PTS HPr component phosphorylation site

Pfam (EcoliWiki Page)

PF00359 Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2

Pfam (EcoliWiki Page)

PF05524 PEP-utilising enzyme, N-terminal

EcoCyc

EcoCyc:EG11906

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11906

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001835

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1851

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012920

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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