ptsA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PtsA

Synonyms

fused predicted PTS enzymes: Hpr component[1], enzyme I component[1], enzyme IIA component[1], B3947[2][1], FrwA[2][1], YijH[2][1], PtsA[2][1] , ECK3939, frwA, JW5555, yijH, b3947

Product description

PEP-protein phosphotransferase system enzyme I[2][3]

Putative phosphotransferase system enzyme I; also has N-terminal Hpr-like domain and C-terminal Enzyme IIA domain[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015813

F

Seeded from EcoCyc (v14.0)

complete

GO:0005215

transporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016152

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000032

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001020

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002178

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005698

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008731

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008731

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001020

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002178

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016152

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0008643

carbohydrate transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0762

P

Seeded from EcoCyc (v14.0)

complete

GO:0008965

phosphoenolpyruvate-protein phosphotransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008731

F

Seeded from EcoCyc (v14.0)

complete

GO:0008965

phosphoenolpyruvate-protein phosphotransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.3.9

F

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000032

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001020

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002178

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005698

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008731

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0598

P

Seeded from EcoCyc (v14.0)

complete

GO:0016301

kinase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc (v14.0)

complete

GO:0016310

phosphorylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000121

P

Seeded from EcoCyc (v14.0)

complete

GO:0016310

phosphorylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008279

P

Seeded from EcoCyc (v14.0)

complete

GO:0016310

phosphorylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018274

P

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0016772

transferase activity, transferring phosphorus-containing groups

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000121

F

Seeded from EcoCyc (v14.0)

complete

GO:0016772

transferase activity, transferring phosphorus-containing groups

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006318

F

Seeded from EcoCyc (v14.0)

complete

GO:0016772

transferase activity, transferring phosphorus-containing groups

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008279

F

Seeded from EcoCyc (v14.0)

complete

GO:0016772

transferase activity, transferring phosphorus-containing groups

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018274

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MALIVEFICE LPNGVHARPA SHVETLCNTF SSQIEWHNLR TDRKGNAKSA LALIGTDTLA
GDNCQLLISG ADEQEAHQRL SQWLRDEFPH CDAPLAEVKS DELEPLPVSL TNLNPQIIRA
RTVCSGSAGG ILTPISSLDL NALGNLPAAK GVDAEQSALE NGLTLVLKNI EFRLLDSDGA
TSAILEAHRS LAGDTSLREH LLAGVSAGLS CAEAIVASAN HFCEEFSRSS SSYLQERALD
VRDVCFQLLQ QIYGEQRFPA PGKLTQPAIC MADELTPSQF LELDKNHLKG LLLKSGGTTS
HTVILARSFN IPTLVGVDID ALTPWQQQTI YIDGNAGAIV VEPGEAVARY YQQEARVQDA
LREQQRVWLT QQARTADGIR IEIAANIAHS VEAQAAFGNG AEGVGLFRTE MLYMDRTSAP
GESELYNIFC QALESANGRS IIVRTMDIGG DKPVDYLNIP AEANPFLGYR AVRIYEEYAS
LFTTQLRSIL RASAHGSLKI MIPMISSMEE ILWVKEKLAE AKQQLRNEHI PFDEKIQLGI
MLEVPSVMFI IDQCCEEIDF FSIGSNDLTQ YLLAVDRDNA KVTRHYNSLN PAFLRALDYA
VQAVHRQGKW IGLCGELGAK GSVLPLLVGL GLDELSMSAP SIPAAKARMA QLDSRECRKL
LNQAMACRTS LEVEHLLAQF RMTQQDAPLV TAECITLESD WRSKEEVLKG MTDNLLLAGR
CRYPRKLEAD LWAREAVFST GLGFSFAIPH SKSEHIEQST ISVARLQAPV RWGDDEAQFI
IMLTLNKHAA GDQHMRIFSR LARRIMHEEF RNALVNAASA DAIASLLQHE LEL
Length

833

Mol. Wt

91.775 kDa

pI

4.4 (calculated)

Extinction coefficient

71,850 - 73,975 (calc based on 15 Y, 9 W, and 17 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Modification Site

669

phosphorylation site at T669

probability less than 75%

PMID:17938405[5]

Modification Site

670

phosphorylation site at S670

probability less than 75%

PMID:17938405[5]

Domain

259..337

PF00391 PEP-utilising enzyme, mobile domain

PMID:19920124[6]

Domain

362..654

PF02896 PEP-utilising enzyme, TIM barrel domain

PMID:19920124[6]

Domain

2..87

PF00381 PTS HPr component phosphorylation site

PMID:19920124[6]

Domain

688..830

PF00359 Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2

PMID:19920124[6]

Domain

118..238

PF05524 PEP-utilising enzyme, N-terminal

PMID:19920124[6]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ptsA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:49176446

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948437

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012920

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P32670

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11906

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11906

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948437

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001835

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1851

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 Macek, B et al. (2008) Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol. Cell Proteomics 7 299-307 PubMed
  6. 6.0 6.1 6.2 6.3 6.4 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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