pth:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pth

Mnemonic

Peptidyl tRNA hydrolase, recombination adept with plasmid

Synonyms

ECK1192, b1204, JW1195, rap, asuA?, G518[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

27.1 minutes 

MG1655: 1257736..1257152
<gbrowseImage> name=NC_000913:1257152..1257736 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1258663..1258079
<gbrowseImage> name=NC_012967:1258079..1258663 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1145873..1146457
<gbrowseImage> name=NC_012759:1145873..1146457 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1260090..1259506
<gbrowseImage> name=NC_007779:1259506..1260090 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1297977..1297393
<gbrowseImage> name=NC_010473:1297393..1297977 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1257152

Edman degradation

PMID:1833189[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

pth::Tn5KAN-2 (FB20267)

Insertion at nt 551 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20267

does not contain pKD46

pthG101D

G101D

Pth(ts) mutants synthesize thermosensitive PTH and die at 42 degrees Celsius from a defect in protein synthesis

seeded from UniProt:P0A7D1

pthR134H

R134H

Rap mutants do not support vegetative growth of bacteriophage lambda and die upon transcription of lambda DNA bar sites

seeded from UniProt:P0A7D1

pth-1

CGSC:12904


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1195

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCACGATTAAATTGATTGTCGG

Primer 2:CCTTGCGCTTTAAAGGCGTGCAA

12A3

Kohara Phage

Genobase

PMID:3038334[6]

4D10

Kohara Phage

Genobase

PMID:3038334[6]

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[7]

est. P1 cotransduction: 43% [8]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[7]

est. P1 cotransduction: 12% [8]
Synonyms:zch-506::Tn10, zci-506::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10785

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10785

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000776

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945765

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0778

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004043

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. García-Villegas, MR et al. (1991) Peptidyl-tRNA hydrolase is involved in lambda inhibition of host protein synthesis. EMBO J. 10 3549-55 PubMed
  4. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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