pstS:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pstS

Mnemonic

P-specific transport

Synonyms

ECK3721, b3728, JW3706, R2pho, nmpA, phoR2a, phoS, phoS,T, t[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

84.24 minutes 

MG1655: 3909548..3908508
<gbrowseImage> name=NC_000913:3908508..3909548 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3871891..3870851
<gbrowseImage> name=NC_012967:3870851..3871891 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3796841..3797881
<gbrowseImage> name=NC_012759:3796841..3797881 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3725156..3726196
<gbrowseImage> name=NC_007779:3725156..3726196 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4007132..4006092
<gbrowseImage> name=NC_010473:4006092..4007132 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3908511

Edman degradation

PMID:6321434[3]
PMID:6365894[4]
PMID:9298646[5]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

pstS(del) (Keio:JW3706)

deletion

deletion

PMID:16738554[6]

Shigen

CGSC10710[7]

phoS-25

CGSC:6395

phoS-28

CGSC:6410

phoS-27

CGSC:6414

phoS-104

CGSC:6822

phoS-65

CGSC:7367

phoS-164

CGSC:7387

phoS-21

CGSC:7987

phoS-3

CGSC:11510

phoS-24

CGSC:42457

pstS605(del)::cat

PMID:10829079[8]

CGSC:65388

pstS625(del)::kan

PMID:10829079[8]

CGSC:65391

pstS624(del)(::FRT)

CGSC:65913

pstS759(del)::kan

PMID:16738554[6]

CGSC:102510

phoS-

Resistant to

Resistance to arsenate

PMID:331084[9]

pstS in strain 10B5

Resistant to

Resistant to Arsenate

PMID:765745[10]

Strain: 10B5

pstS in strain UR1

Resistant to

Resistant to Arsenate

PMID:4570598[11]

Strain: UR1


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3706

Plasmid clone

Shigen

PMID:16769691[12]

Status:Clone OK

Primer 1:GCCAAAGTTATGCGTACCACCGT

Primer 2:CCGTACAGCGGCTTACCGCTACT

2A1

Kohara Phage

Genobase

PMID:3038334[13]

7A1

Kohara Phage

Genobase

PMID:3038334[13]

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[7]

est. P1 cotransduction: 25% [14]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[7]

est. P1 cotransduction: 46% [14]
Synonyms:zie-296::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10734

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10734

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000725

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948237

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0727

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012190

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Surin, BP et al. (1984) Structural gene for the phosphate-repressible phosphate-binding protein of Escherichia coli has its own promoter: complete nucleotide sequence of the phoS gene. J. Bacteriol. 157 772-8 PubMed
  4. Magota, K et al. (1984) Nucleotide sequence of the phoS gene, the structural gene for the phosphate-binding protein of Escherichia coli. J. Bacteriol. 157 909-17 PubMed
  5. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  6. 6.0 6.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  7. 7.0 7.1 7.2 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed
  9. Yagil, E & Be'eri, H (1977) Arsenate-resistant alkaline phosphatase-constitutive mutants of Escherichia coli. Mol. Gen. Genet. 154 185-9 PubMed
  10. Sprague, GF Jr et al. (1975) A mutant of Escherichia coli auxotrophic for organic phosphates: evidence for two defects in inorganic phosphate transport. Mol. Gen. Genet. 143 71-7 PubMed
  11. Willsky, GR et al. (1973) Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation. J. Bacteriol. 113 529-39 PubMed
  12. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  13. 13.0 13.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  14. 14.0 14.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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