prc:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
prc |
|---|---|
| Mnemonic |
PBP protease, C terminal |
| Synonyms |
ECK1829, b1830, JW1819, tsp[1], tsp |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
41.18 minutes |
MG1655: 1912840..1910792 |
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NC_012967: 1893393..1891345 |
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NC_012759: 1802851..1804899 |
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W3110 |
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W3110: 1916530..1914482 |
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DH10B: 2003411..2001363 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
1910795 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
prc(del) (Keio:JW1819) |
deletion |
deletion |
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prc::Tn5KAN-2 (FB20464) |
Insertion at nt 405 in Plus orientation |
contains pKD46 | |||||
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prcK477R |
K477R |
Loss of activity. Perturbs protein structure |
seeded from UniProt:P23865 | ||||
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prcK477A,H |
K477A,H |
Loss of activity. No apparent effect on protein structure |
seeded from UniProt:P23865 | ||||
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prcG397A |
G397A |
Loss of activity. Perturbs protein structure |
seeded from UniProt:P23865 | ||||
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prcG398A |
G398A |
Loss of activity. Perturbs protein structure |
seeded from UniProt:P23865 | ||||
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prcS452A |
S452A |
Loss of activity |
seeded from UniProt:P23865 | ||||
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prcS452C |
S452C |
Reduces activity by over 90% |
seeded from UniProt:P23865 | ||||
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prcE455A |
E455A |
Loss of activity. Perturbs protein structure |
seeded from UniProt:P23865 | ||||
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prcD463A |
D463A |
Loss of activity |
seeded from UniProt:P23865 | ||||
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prcD463N |
D463N |
Reduces activity by 90% |
seeded from UniProt:P23865 | ||||
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prcT474A |
T474A |
Loss of activity. Perturbs protein structure |
seeded from UniProt:P23865 | ||||
|
prc-755(del)::kan |
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prc755(del)::FRT |
Mutagenesis rate |
Decreased stress-induced mutagenesis (SIM) phenotype. |
Parental Strain: SMR4562 Experimental Strain: SMR12251 |
Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population. | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1819 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAACATGTTTTTTAGGCTTAC Primer 2:CCtTTGACGGGAGCGGGTTGTTC | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 17% [9] | ||
|
Linked marker |
est. P1 cotransduction: 50% [9] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Silber, KR et al. (1992) Tsp: a tail-specific protease that selectively degrades proteins with nonpolar C termini. Proc. Natl. Acad. Sci. U.S.A. 89 295-9 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
