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Standard Name	pphA
Gene Synonym(s)	ECK1837, b1838, JW1827, prpA, yebX^[1], yebX
Product Desc.	protein phosphatase 1 modulates phosphoproteins, signals protein misfolding^[2]^[3] Ser/Thr protein phosphatase 1; signals periplasmic protein misfolding; heat shock regulon^[4]
Product Synonyms(s)	serine/threonine-specific protein phosphatase 1^[1], B1838^[2]^[1], PrpA^[2]^[1], YebX^[2]^[1], PphA^[2]^[1] , ECK1837, JW1827, prpA, yebX, b1838
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pphA^[2], prpA
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	pphA
Mnemonic	Phosphoprotein phosphatase
Synonyms	ECK1837, b1838, JW1827, prpA, yebX^[1], yebX
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	41.39 minutes, 41.39 minutes	MG1655: 1920993..1920337 <gbrowseImage> name=NC_000913:1920337..1920993 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1901546..1900890 <gbrowseImage> name=NC_012967:1900890..1901546 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1812396..1813052 <gbrowseImage> name=NC_012759:1812396..1813052 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1924683..1924027 <gbrowseImage> name=NC_007779:1924027..1924683 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2011564..2010908 <gbrowseImage> name=NC_010473:2010908..2011564 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔpphA (Keio:JW1827)	deletion	deletion		PMID:16738554	Shigen CGSC9524^[5]
pphAD24V		D24V	Loss of function			seeded from UniProt:P55798
pphAH26L		H26L	Loss of function			seeded from UniProt:P55798
pphAH26N		H26N	Loss of function			seeded from UniProt:P55798
pphAL107Q		L107Q	Loss of function			seeded from UniProt:P55798
prpA1					CGSC:7587
prpA3					CGSC:7595
prpA12					CGSC:10846
ΔpphA763::kan				PMID:16738554	CGSC:102424
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1827	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAACAGCCTGCGCCCGTTTA Primer 2:CCTTGCAATTGCACAAGAGTCAG
19H3	Kohara Phage	Genobase	PMID:3038334
zea-225::Tn10	Linked marker	CAG18465 = CGSC7388^[5]	est. P1 cotransduction: 9% ^[6] Synonyms:zdj-225::Tn10
eda-51::Tn10	Linked marker	CAG18486 = CGSC7392^[5]	est. P1 cotransduction: 72% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7011	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14026	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003598	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946356	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3780	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006114	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PphA
Synonyms	serine/threonine-specific protein phosphatase 1^[1], B1838^[2]^[1], PrpA^[2]^[1], YebX^[2]^[1], PphA^[2]^[1] , ECK1837, JW1827, prpA, yebX, b1838
Product description	protein phosphatase 1 modulates phosphoproteins, signals protein misfolding^[2]^[3] Ser/Thr protein phosphatase 1; signals periplasmic protein misfolding; heat shock regulon^[4]
EC number (for enzymes)	3.1.3.16^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004721	phosphoprotein phosphatase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.1.3.16	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004721	phosphoprotein phosphatase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0904	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005506	iron ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004843	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006186	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030145	manganese ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0464	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	groL		PMID:16606699	Experiment(s):EBI-1140955
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMKQPAPVYQ RIAGHQWRHI WLSGDIHGCL EQLRRKLWHC RFDPWRDLLI SVGDVIDRGP QSLRCLQLLE QHWVCAVRGN HEQMAMDALA SQQMSLWLMN GGDWFIALAD NQQKQAKTAL EKCQHLPFIL EVHSRTGKHV IAHADYPDDV YEWQKDVDLH QVLWSRSRLG ERQKGQGITG ADHFWFGHTP LRHRVDIGNL HYIDTGAVFG GELTLVQLQ
Length	219
Mol. Wt	25.404 kDa
pI	7.7 (calculated)
Extinction coefficient	60,960 - 61,585 (calc based on 4 Y, 10 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	17..205	PF00149 Calcineurin-like phosphoesterase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pphA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111344	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946356	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006114	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P55798	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7011	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14026	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946356	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003598	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3780	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	8^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	13	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	8^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pphA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1920973..1921013 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1838 (EcoliWiki Page)	NCBI GEO profiles for pphA
microarray	GenExpDB:b1838 (EcoliWiki Page)	Summary of data for pphA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1920922..1921435) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ17; Well:G7^[7]
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Notes

Accessions Related to pphA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7011	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3780	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1838
EcoGene	EcoGene:EG14026	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003598	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006114	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Homo sapiens	ENSP00000341648 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000007531 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	PPHA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00149 Calcineurin-like phosphoesterase
Panther (EcoliWiki Page)	PTHR11668:SF153
Superfamily (EcoliWiki Page)	SUPERFAMILY:56300
PFAM (EcoliWiki Page)	PFAM:PF00149
EcoCyc	EcoCyc:G7011	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14026	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003598	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3780	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006114	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

pphA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to pphA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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