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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	potG
Gene Synonym(s)	ECK0846, b0855, JW5818^[1], JW5818
Product Desc.	PotG^[2]^[3]; Component of putrescine ABC transporter^[2]^[3] Putrescine ABC transporter ATP-binding protein^[4]
Product Synonyms(s)	putrescine transporter subunit: ATP-binding component of ABC superfamily^[1], B0855^[2]^[1], PotG^[2]^[1] , ECK0846, JW5818, b0855
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): potFGHI^[2]
Regulation/Activity
Quick Links
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Notes

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PotIHGF putrescine ABC transport system.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	potG
Mnemonic	Putrescine-ornithine transporter
Synonyms	ECK0846, b0855, JW5818^[1], JW5818
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	19.27 minutes	MG1655: 894214..895347 <gbrowseImage> name=NC_000913:894214..895347 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 911698..912831 <gbrowseImage> name=NC_012967:911698..912831 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 797182..798315 <gbrowseImage> name=NC_012759:797182..798315 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 895413..896546 <gbrowseImage> name=NC_007779:895413..896546 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 948142..949275 <gbrowseImage> name=NC_010473:948142..949275 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔpotG (Keio:JW5818)	deletion	deletion	PMID:16738554	Shigen
potG::Tn5KAN-2 (FB20243)	Insertion at nt 1110 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20243	does not contain pKD46
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5818	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAATGACGCTATCCCTCGCCC Primer 2:CCAACCGTCAGCACCACACAGCT
5F4	Kohara Phage	Genobase	PMID:3038334
zbh-29::Tn10	Linked marker	CAG18493 = CGSC7353^[5]	est. P1 cotransduction: 1% ^[6] Synonyms:zbi-29::Tn10
zca-1230::Tn10	Linked marker	CAG18478 = CGSC7356^[5]	est. P1 cotransduction: 8% ^[6] Synonyms:zbj-1230::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11630	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11630	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001578	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945476	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1587	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002915	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PotG
Synonyms	putrescine transporter subunit: ATP-binding component of ABC superfamily^[1], B0855^[2]^[1], PotG^[2]^[1] , ECK0846, JW5818, b0855
Product description	PotG^[2]^[3]; Component of putrescine ABC transporter^[2]^[3] Putrescine ABC transporter ATP-binding protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003593	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003439	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008995	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013611	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017871	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	PMID:8416922			F	Seeded from EcoCyc (v14.0)	Missing: evidence
	GO:0005737	cytoplasm	PMID:8416922			C	Seeded from EcoCyc (v14.0)	Missing: evidence
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0015594	putrescine-importing ATPase activity	PMID:8416922	IMP: Inferred from Mutant Phenotype		F	Seeded from EcoCyc (v14.0)	complete
	GO:0015594	putrescine-importing ATPase activity	PMID:8416922			F	Seeded from EcoCyc (v14.0)	Missing: evidence
	GO:0015847	putrescine transport	PMID:8416922	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005893	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0019898	extrinsic to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9903	C	Seeded from EcoCyc (v14.0)	complete
	GO:0043190	ATP-binding cassette (ABC) transporter complex	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008995	C	Seeded from EcoCyc (v14.0)	complete
	GO:0043190	ATP-binding cassette (ABC) transporter complex	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013611	C	Seeded from EcoCyc (v14.0)	complete
	GO:0043190	ATP-binding cassette (ABC) transporter complex	PMID:8416922			C	Seeded from EcoCyc (v14.0)	Missing: evidence
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of putrescine ABC transporter	could be indirect
Protein	yrfF		PMID:19402753	LCMS(ID Probability):98.7
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MPEGRTTPAG NSHHYGALAH IQCRRAAVND AIPRPQAKTR KALTPLLEIR NLTKSYDGQH AVDDVSLTIY KGEIFALLGA SGCGKSTLLR MLAGFEQPSA GQIMLDGVDL SQVPPYLRPI NMMFQSYALF PHMTVEQNIA FGLKQDKLPK AEIASRVNEM LGLVHMQEFA KRKPHQLSGG QRQRVALARS LAKRPKLLLL DEPMGALDKK LRDRMQLEVV DILERVGVTC VMVTHDQEEA MTMAGRIAIM NRGKFVQIGE PEEIYEHPTT RYSAEFIGSV NVFEGVLKER QEDGLVLDSP GLVHPLKVDA DASVVDNVPV HVALRPEKIM LCEEPPANGC NFAVGEVIHI AYLGDLSVYH VRLKSGQMIS AQLQNAHRHR KGLPTWGDEV RLCWEVDSCV VLTV
Length	404
Mol. Wt	44.783 kDa
pI	7.4 (calculated)
Extinction coefficient	24,410 - 25,285 (calc based on 9 Y, 2 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	59..178	PF00005 ABC transporter	PMID:19920124
Domain	295..375	PF08402 TOBE domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=potG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111177	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945476	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002915	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P31134	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11630	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11630	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945476	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001578	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1587	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	75	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	223	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	21^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
potFGHI
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:894194..894234 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0855 (EcoliWiki Page)	NCBI GEO profiles for potG
microarray	GenExpDB:b0855 (EcoliWiki Page)	Summary of data for potG from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to potG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11630	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1587	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0855
EcoGene	EcoGene:EG11630	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001578	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002915	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000022559 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0219981 (score: 1.000; bootstrap: 52%) DDB0215337 (score: 0.090) DDB0201653 (score: 0.088) DDB0191529 (score: 0.074)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0031362 (score: 1.000; bootstrap: 69%) FBgn0032167 (score: 0.229) FBgn0025683 (score: 0.225) FBgn0020762 (score: 0.211) FBgn0031516 (score: 0.208) FBgn0031220 (score: 0.184) FBgn0052091 (score: 0.175) FBgn0031449 (score: 0.159) FBgn0031515 (score: 0.153) FBgn0003996 (score: 0.077) FBgn0003515 (score: 0.074) FBgn0020445 (score: 0.059)	From Inparanoid:20070104
Drosophila pseudoobscura	GA14594-PA (score: 1.000; bootstrap: 57%) GA16356-PA (score: 0.236) GA15545-PA (score: 0.235) GA19180-PA (score: 0.225) GA18458-PA (score: 0.185) GA16671-PA (score: 0.176) GA16397-PA (score: 0.154) GA15454-PA (score: 0.057)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000130824 (score: 1.000; bootstrap: 57%) NEWSINFRUP00000130826 (score: 0.180) NEWSINFRUP00000167567 (score: 0.137) NEWSINFRUP00000182359 (score: 0.125) NEWSINFRUP00000177047 (score: 0.125) NEWSINFRUP00000168237 (score: 0.097) NEWSINFRUP00000171182 (score: 0.092)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00014344001 (score: 1.000; bootstrap: 57%) GSTENP00014343001 (score: 0.105) GSTENP00020877001 (score: 0.101) GSTENP00038511001 (score: 0.091) GSTENP00014937001 (score: 0.051)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000050780 (score: 1.000; bootstrap: 75%)	From Inparanoid:20070104
Shigella flexneri	POTG	From SHIGELLACYC
E. coli O157	POTG	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00005 ABC transporter
Superfamily (EcoliWiki Page)	SUPERFAMILY:50331
Superfamily (EcoliWiki Page)	SUPERFAMILY:52540
Pfam (EcoliWiki Page)	PF08402 TOBE domain
EcoCyc	EcoCyc:EG11630	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11630	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001578	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1587	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002915	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

potG:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to potG Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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