polB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

polB

Gene Synonym(s)

ECK0061, b0060, JW0059, dinA[1], dinA

Product Desc.

DNA polymerase II[2][3]

DNA polymerase II, capable of translesion synthesis; role in the resumption of DNA synthesis after UV irradiation[4]

Product Synonyms(s)

DNA polymerase II[1], B0060[2][1], DinA[2][1], PolB[2][1] , dinA, ECK0061, JW0059, b0060

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): polB[2], dinA

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

polB

Mnemonic

Polymerase

Synonyms

ECK0061, b0060, JW0059, dinA[1], dinA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

1.37 minutes 

MG1655: 65780..63429
<gbrowseImage> name=NC_000913:63429..65780 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 68584..66233
<gbrowseImage> name=NC_012967:66233..68584 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 63429..65780
<gbrowseImage> name=NC_012759:63429..65780 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 65780..63429
<gbrowseImage> name=NC_007779:63429..65780 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

63432

Edman degradation

PMID:2034216
PMID:2217198


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

polB(del) (Keio:JW0059)

deletion

deletion

PMID:16738554

Shigen

polBG401D

G401D

(in allele POLB100)

Strain variation; seeded from UniProt:P21189

polB100

CGSC:6869

(araD-polB)(del)::omega

araD-polB was deleted and replaced with a omega cassette

rate of DNA replication

There was a significant delay in the resumption of replication.

PMID:10430924

See Figure 2.

polB(del)umuDC(del)

Deletion of araD-polB operon and replaced with omega cassette. Deletion of umuDC operon and replaced with chloramephenicol acetylransferase gene (cat)

Sensitivity to

Sensitivity toward UV radiation was increased by 2 to 3 fold.

PMID:10430924

See Figure 1.

polB(del)umuDC(del)

Deletion of araD-polB operon and replaced with omega cassette. Deletion of umuDC operon and replaced with chloramephenicol acetylransferase gene (cat)

rate of replication

There was a significant delay in the resumption of DNA replication after exposure to UV radiation.

PMID:10430924

See Figure 3.

dnaE486 (araD-polB)(del)::omega

Deletion of araD-polB operon and replaced with omega cassette. Fusion with rapid stop allele dnaE846

Sensitivity to

There is a significant delay in the resumption of DNA replication after exposure to UV radiation at 30 C

PMID:10430924

See figure 4 B.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0059

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCGCAGGCAGGTTTTATCTT

Primer 2:CCAAATAGCCCAAGTTGCCCGGT

8D2

Kohara Phage

Genobase

PMID:3038334

5H5

Kohara Phage

Genobase

PMID:3038334

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 30% [6]
Synonyms:car-96::Tn10

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[5]

est. P1 cotransduction: 48% [6]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10747

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10747

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000738

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944779

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0740

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000202

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PolB

Synonyms

DNA polymerase II[1], B0060[2][1], DinA[2][1], PolB[2][1] , dinA, ECK0061, JW0059, b0060

Product description

DNA polymerase II[2][3]

DNA polymerase II, capable of translesion synthesis; role in the resumption of DNA synthesis after UV irradiation[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006134

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006172

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017964

F

Seeded from EcoCyc (v14.0)

complete

GO:0009314

response to radiation

P

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006172

F

Seeded from EcoCyc (v14.0)

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012337

F

Seeded from EcoCyc (v14.0)

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017964

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006134

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003887

DNA-directed DNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006133

F

Seeded from EcoCyc (v14.0)

complete

GO:0003887

DNA-directed DNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006134

F

Seeded from EcoCyc (v14.0)

complete

GO:0003887

DNA-directed DNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006172

F

Seeded from EcoCyc (v14.0)

complete

GO:0003887

DNA-directed DNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017964

F

Seeded from EcoCyc (v14.0)

complete

GO:0003887

DNA-directed DNA polymerase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.7.7

F

Seeded from EcoCyc (v14.0)

complete

GO:0003887

DNA-directed DNA polymerase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0239

F

Seeded from EcoCyc (v14.0)

complete

GO:0006139

nucleobase, nucleoside, nucleotide and nucleic acid metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006172

P

Seeded from EcoCyc (v14.0)

complete

GO:0006139

nucleobase, nucleoside, nucleotide and nucleic acid metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017964

P

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006133

P

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006134

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc (v14.0)

complete

GO:0009432

SOS response

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0742

P

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0016779

nucleotidyltransferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0548

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

sdhA

PMID:16606699

Experiment(s):EBI-1135463

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MAQAGFILTR HWRDTPQGTE VSFWLATDNG PLQVTLAPQE SVAFIPADQV PRAQHILQGE
QGFRLTPLAL KDFHRQPVYG LYCRAHRQLM NYEKRLREGG VTVYEADVRP PERYLMERFI
TSPVWVEGDM HNGTIVNARL KPHPDYRPPL KWVSIDIETT RHGELYCIGL EGCGQRIVYM
LGPENGDASS LDFELEYVAS RPQLLEKLNA WFANYDPDVI IGWNVVQFDL RMLQKHAERY
RLPLRLGRDN SELEWREHGF KNGVFFAQAK GRLIIDGIEA LKSAFWNFSS FSLETVAQEL
LGEGKSIDNP WDRMDEIDRR FAEDKPALAT YNLKDCELVT QIFHKTEIMP FLLERATVNG
LPVDRHGGSV AAFGHLYFPR MHRAGYVAPN LGEVPPHASP GGYVMDSRPG LYDSVLVLDY
KSLYPSIIRT FLIDPVGLVE GMAQPDPEHS TEGFLDAWFS REKHCLPEIV TNIWHGRDEA
KRQGNKPLSQ ALKIIMNAFY GVLGTTACRF FDPRLASSIT MRGHQIMRQT KALIEAQGYD
VIYGDTDSTF VWLKGAHSEE EAAKIGRALV QHVNAWWAET LQKQRLTSAL ELEYETHFCR
FLMPTIRGAD TGSKKRYAGL IQEGDKQRMV FKGLETVRTD WTPLAQQFQQ ELYLRIFRNE
PYQEYVRETI DKLMAGELDA RLVYRKRLRR PLSEYQRNVP PHVRAARLAD EENQKRGRPL
QYQNRGTIKY VWTTNGPEPL DYQRSPLDYE HYLTRQLQPV AEGILPFIED NFATLMTGQL GLF
Length

783

Mol. Wt

90.054 kDa

pI

6.9 (calculated)

Extinction coefficient

137,170 - 138,045 (calc based on 33 Y, 16 W, and 7 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P21189

Domain

373..440

PF00136 DNA polymerase family B

PMID:19920124

Domain

444..779

PF00136 DNA polymerase family B

PMID:19920124

Domain

49..296

PF03104 DNA polymerase family B, exonuclease domain

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=polB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128054

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944779

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000202

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P21189

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10747

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10747

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944779

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000738

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0740

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

32

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

16

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

9a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

polB

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:65760..65800 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0060 (EcoliWiki Page)

NCBI GEO profiles for polB

microarray

GenExpDB:b0060 (EcoliWiki Page)

Summary of data for polB from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (65704..65932) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ20; Well:E6[7]

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Notes

Accessions Related to polB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10747

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0740

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0060

EcoGene

EcoGene:EG10747

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000738

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000202

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000018383 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000022139 (score: 1.000; bootstrap: 76%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G63960 (score: 1.000; bootstrap: 89%)
  • AT1G67500 (score: 0.090)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000010742 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00041727 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00012936 (score: 1.000; bootstrap: 78%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000019936 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000005791 (score: 1.000; bootstrap: 100%)
  • ENSCINP00000015719 (score: 0.108)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-030114-9 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0232274 (score: 1.000; bootstrap: 100%)
  • DDB0232268 (score: 1.000; bootstrap: 100%)
  • DDB0232271 (score: 0.096)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0004493 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA19530-PA (score: 1.000; bootstrap: 85%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000026266 (score: 1.000; bootstrap: 88%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000314415 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000028348 (score: 1.000; bootstrap: 53%)
  • ENSMMUP00000028356 (score: 0.109)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000009125 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:97741 (score: 1.000; bootstrap: 100%)
  • MGI:1337131 (score: 0.125)

From Inparanoid:20070104

Oryza gramene

  • Q9LRE6 (score: 1.000; bootstrap: 82%)
  • Q53P50 (score: 1.000; bootstrap: 84%)
  • Q53NH0 (score: 0.518)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000019473 (score: 1.000; bootstrap: 62%)
  • ENSPTRP00000031607 (score: 0.062)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000026797 (score: 1.000; bootstrap: 100%)
  • ENSRNOP00000000725 (score: 0.119)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YDL102W (score: 1.000; bootstrap: 100%)
  • YPL167C (score: 0.085)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC3364 (score: 1.000; bootstrap: 100%)
  • SPAC6880 (score: 0.077)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000160398 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00021892001 (score: 1.000; bootstrap: 97%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000047196 (score: 1.000; bootstrap: 77%)

From Inparanoid:20070104

Shigella flexneri

POLB

From SHIGELLACYC

E. coli O157

POLB

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF03104 DNA polymerase family B, exonuclease domain

Pfam (EcoliWiki Page)

PF00136 DNA polymerase family B

Pfam (EcoliWiki Page)

PF00136 DNA polymerase family B

Superfamily (EcoliWiki Page)

SUPERFAMILY:53098

Panther (EcoliWiki Page)

PTHR10322:SF12

Superfamily (EcoliWiki Page)

SUPERFAMILY:56672

EcoCyc

EcoCyc:EG10747

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10747

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000738

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0740

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000202

Escherichia coli str. K-12 substr. MG1655

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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