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Standard Name	pmbA
Gene Synonym(s)	ECK4230, b4235, JW4194, mcb, tldE^[1], tldE
Product Desc.	protease involved in Microcin B17 maturation and in sensitivity to the DNA gyrase inhibitor LetD^[2]^[3] Probable peptidase required for the maturation and secretion of the antibiotic peptide MccB17; involved in CcdA antidote degradation; tolerance for effects on DNA gyrase by sex factor F gene letD^[4]
Product Synonyms(s)	predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17^[1], B4235^[2]^[1], Mcb^[2]^[1], TldE^[2]^[1], PmbA^[2]^[1] , ECK4230, JW4194, mcb, tldE, b4235
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pmbA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	pmbA
Mnemonic	Peptide MccB17
Synonyms	ECK4230, b4235, JW4194, mcb, tldE^[1], tldE
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	96.04 minutes	MG1655: 4455982..4457334 <gbrowseImage> name=NC_000913:4455982..4457334 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4441210..4442562 <gbrowseImage> name=NC_012967:4441210..4442562 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4394717..4396069 <gbrowseImage> name=NC_012759:4394717..4396069 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4462639..4463991 <gbrowseImage> name=NC_007779:4462639..4463991 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4557790..4559142 <gbrowseImage> name=NC_010473:4557790..4559142 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔpmbA (Keio:JW4194)	deletion	deletion	PMID:16738554	Shigen CGSC11017^[5]
ΔpmbA782::kan			PMID:16738554	CGSC:102382
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4194	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCACTTGCAATGAAAGTAAT Primer 2:CCtTGTCCGGCGATTTTCATCTC
zjf-920::Tn10	Linked marker	CAG12019 = CGSC7484^[5]	est. P1 cotransduction: 68% ^[6] Synonyms:zjh-901::Tn10, zjg-920::Tn10
mdoB202::Tn10	Linked marker	CAG18430 = CGSC7488^[5]	est. P1 cotransduction: % ^[6] Synonyms:zjj-202::Tn10, zji-202::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10741	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10741	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000732	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948750	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0734	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013854	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PmbA
Synonyms	predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17^[1], B4235^[2]^[1], Mcb^[2]^[1], TldE^[2]^[1], PmbA^[2]^[1] , ECK4230, JW4194, mcb, tldE, b4235
Product description	protease involved in Microcin B17 maturation and in sensitivity to the DNA gyrase inhibitor LetD^[2]^[3] Probable peptidase required for the maturation and secretion of the antibiotic peptide MccB17; involved in CcdA antidote degradation; tolerance for effects on DNA gyrase by sex factor F gene letD^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	yjiR	PMID:16606699	Experiment(s):EBI-1147715
Protein	idnR	PMID:16606699	Experiment(s):EBI-1147715
Protein	argF	PMID:16606699	Experiment(s):EBI-1147715
Protein	groL	PMID:16606699	Experiment(s):EBI-1147715
Protein	argI	PMID:16606699	Experiment(s):EBI-1147715
Protein	argR	PMID:19402753	LCMS(ID Probability):99.2
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MALAMKVISQ VEAQRKILEE AVSTALELAS GKSDGAEVAV SKTTGISVST RYGEVENVEF NSDGALGITV YHQNRKGSAS STDLSPQAIA RTVQAALDIA RYTSPDPCAG VADKELLAFD APDLDLFHPA EVSPDEAIEL AARAEQAALQ ADKRITNTEG GSFNSHYGVK VFGNSHGMLQ GYCSTRHSLS SCVIAEENGD MERDYAYTIG RAMSDLQTPE WVGADCARRT LSRLSPRKLS TMKAPVIFAN EVATGLFGHL VGAIAGGSVY RKSTFLLDSL GKQILPDWLT IEEHPHLLKG LASTPFDSEG VRTERRDIIK DGILTQWLLT SYSARKLGLK STGHAGGIHN WRIAGQGLSF EQMLKEMGTG LVVTELMGQG VSAITGDYSR GAAGFWVENG EIQYPVSEIT IAGNLKDMWR NIVTVGNDIE TRSNIQCGSV LLPEMKIAGQ
Length	450
Mol. Wt	48.369 kDa
pI	5.4 (calculated)
Extinction coefficient	49,390 - 50,015 (calc based on 11 Y, 6 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	34..327	PF01523 Putative modulator of DNA gyrase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pmbA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16132057	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948750	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013854	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AFK0	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10741	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10741	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948750	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000732	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0734	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	4.35E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	2444	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	615	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1353	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pmbA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4455962..4456002 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4235 (EcoliWiki Page)	NCBI GEO profiles for pmbA
microarray	GenExpDB:b4235 (EcoliWiki Page)	Summary of data for pmbA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4455170..4455403) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ08; Well:H11^[7]
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Notes

Accessions Related to pmbA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10741	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0734	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4235
EcoGene	EcoGene:EG10741	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000732	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013854	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000010763 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	PMBA	From SHIGELLACYC
E. coli O157	PMBA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:111283
Pfam (EcoliWiki Page)	PF01523 Putative modulator of DNA gyrase
EcoCyc	EcoCyc:EG10741	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10741	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000732	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0734	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013854	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

pmbA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to pmbA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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