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Expression|

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Standard Name	plsX
Gene Synonym(s)	ECK1076, b1090, JW5156^[1], JW5156
Product Desc.	fatty acid/phospholipid synthesis protein^[2]^[3] Probable phosphate:acyl-ACP acyltransferase; confers glycerol phosphate auxotrophy, when combined with a plsB Km missense mutant^[4]
Product Synonyms(s)	fatty acid/phospholipid synthesis protein^[1], B1090^[2]^[1], PlsX^[2]^[1] , ECK1076, JW5156, b1090
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): plsX-fabHDG^[2], rpmF-plsX-fabHDG^[2]
Regulation/Activity
Quick Links
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Notes

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PlsX is homologous to the S. pneumoniae phosphate:acyl-ACP acyltransferase PlsX (UniProtKB:Q97TA6) (Lu, 2006). ygiH(plsY) and plsX are synthetic lethals (Yoshimura, 2007). S. pneumoniae PlsX and PlsY work together as a two-step glycerol-3-phosphate acylation, the first step in phosphatidic acid synthesis, an intermediate in phospholipid synthesis (Lu, 2006). PlsXY homologs are present in E. coli, but PlsXY apparently cannot substitute for the single step reaction catalyzed by PlsB, which is essental in E. coli.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	plsX
Mnemonic	Phospholipid synthesis
Synonyms	ECK1076, b1090, JW5156^[1], JW5156
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	24.72 minutes	MG1655: 1146844..1147914 <gbrowseImage> name=NC_000913:1146844..1147914 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1162190..1163260 <gbrowseImage> name=NC_012967:1162190..1163260 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1050769..1051839 <gbrowseImage> name=NC_012759:1050769..1051839 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1149198..1150268 <gbrowseImage> name=NC_007779:1149198..1150268 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1202289..1203359 <gbrowseImage> name=NC_010473:1202289..1203359 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔplsX (Keio:JW5156)	deletion	deletion	PMID:16738554	Shigen CGSC11211^[5]
plsX::Tn5KAN-2 (FB20100)	Insertion at nt 409 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20100	does not contain pKD46
plsX50			PMID:6094487	CGSC:11834
ΔplsX757::kan			PMID:16738554	CGSC:102380
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5156	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACACGTCTAACCCTGGCGTT Primer 2:CCaCGCAGAGTTCCGCTTTTGCC
14C1	Kohara Phage	Genobase	PMID:3038334
zcc-282::Tn10	Linked marker	CAG18466 = CGSC7359^[5]	est. P1 cotransduction: % ^[6]
zce-726::Tn10	Linked marker	CAG12078 = CGSC7361^[5]	est. P1 cotransduction: 75% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11437	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11437	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001401	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946165	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1407	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003692	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PlsX
Synonyms	fatty acid/phospholipid synthesis protein^[1], B1090^[2]^[1], PlsX^[2]^[1] , ECK1076, JW5156, b1090
Product description	fatty acid/phospholipid synthesis protein^[2]^[3] Probable phosphate:acyl-ACP acyltransferase; confers glycerol phosphate auxotrophy, when combined with a plsB Km missense mutant^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003664	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006633	fatty acid biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00019	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006633	fatty acid biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003664	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006633	fatty acid biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012281	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008654	phospholipid biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0594	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016747	transferase activity, transferring acyl groups other than amino-acyl groups	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.3.1	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	lysS		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MGGDFGPSVT VPAALQALNS NSQLTLLLVG NSDAITPLLA KADFEQRSRL QIIPAQSVIA SDARPSQAIR ASRGSSMRVA LELVKEGRAQ ACVSAGNTGA LMGLAKLLLK PLEGIERPAL VTVLPHQQKG KTVVLDLGAN VDCDSTMLVQ FAIMGSVLAE EVVEIPNPRV ALLNIGEEEV KGLDSIRDAS AVLKTIPSIN YIGYLEANEL LTGKTDVLVC DGFTGNVTLK TMEGVVRMFL SLLKSQGEGK KRSWWLLLLK RWLQKSLTRR FSHLNPDQYN GACLLGLRGT VIKSHGAANQ RAFAVAIEQA VQAVQRQVPQ RIAARLESVY PAGFELLDGG KSGTLR
Length	346
Mol. Wt	37.1 kDa
pI	9.6 (calculated)
Extinction coefficient	22,460 - 22,960 (calc based on 4 Y, 3 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	4..331	PF02504 Fatty acid synthesis protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=plsX taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111212	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946165	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003692	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P27247	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11437	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11437	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946165	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001401	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1407	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	18.22+/-0.084	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.211682243	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	204	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	117	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	217	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
plsX-fabHDG
rpmF-plsX-fabHDG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1146824..1146864 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1090 (EcoliWiki Page)	NCBI GEO profiles for plsX
microarray	GenExpDB:b1090 (EcoliWiki Page)	Summary of data for plsX from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1146691..1146892) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ09; Well:D4^[7]
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Notes

Accessions Related to plsX Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11437	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1407	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1090
EcoGene	EcoGene:EG11437	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001401	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003692	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000033246 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	PLSX	From SHIGELLACYC
E. coli O157	PLSX	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02504 Fatty acid synthesis protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:53659
EcoCyc	EcoCyc:EG11437	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11437	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001401	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1407	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003692	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

plsX:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to plsX Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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