pliG:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pliG

Mnemonic

Systematic nomenclature

Synonyms

ycgK, ECK1166, b1178, JW1167[1], JW1167

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

26.43 minutes 

MG1655: 1226695..1226294
<gbrowseImage> name=NC_000913:1226294..1226695 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1227633..1227232
<gbrowseImage> name=NC_012967:1227232..1227633 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1115015..1115416
<gbrowseImage> name=NC_012759:1115015..1115416 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1229049..1228648
<gbrowseImage> name=NC_007779:1228648..1229049 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1266936..1266535
<gbrowseImage> name=NC_010473:1266535..1266936 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔycgK (Keio:JW1167)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9076[3]

ΔycgK::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938[4]

ΔycgK::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938[4]

ΔycgK767::kan

PMID:16738554[2]

CGSC:104022


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1167

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAAATCAAGAGCATCAGGAA

Primer 2:CCtTTAATTTGAATATCGACGTT

zcf-117::Tn10

Linked marker

CAG18463 = CGSC7363[3]

est. P1 cotransduction: 58% [6]

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[3]

est. P1 cotransduction: 77% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6615

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13892

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003210

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946963

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3651

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003948

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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