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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	phoB
Gene Synonym(s)	ECK0393, b0399, JW0389, phoRc, phoT^[1]
Product Desc.	PhoB transcriptional dual regulator^[2]^[3] Positive response regulator for pho regulon, two-component system^[4]
Product Synonyms(s)	B0399^[2], PhoT^[2], positive response regulator for pho regulon (and asr gene), sensor is PhoR (or CreC)^[2], phosphate regulon transcriptional regulatory protein PhoB^[2], , ECK0393, JW0389, phoRc, b0399
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): phoBR^[2], OP00072, phoB
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	phoB
Mnemonic	Phosphate
Synonyms	ECK0393, b0399, JW0389, phoRc, phoT^[1]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	8.97 minutes	MG1655: 416366..417055 <gbrowseImage> name=NC_000913:416366..417055 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 385565..386254 <gbrowseImage> name=NC_012967:385565..386254 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 319125..319814 <gbrowseImage> name=NC_012759:319125..319814 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 416366..417055 <gbrowseImage> name=NC_007779:416366..417055 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 355697..356386 <gbrowseImage> name=NC_010473:355697..356386 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Transcription start site	416325	source: RegulonDB
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
phoB(del) (Keio:JW0389)	deletion	deletion			PMID:16738554	Shigen CGSC8565^[5]
phoB62						CGSC:7691
phoB52						CGSC:7693
phoB54						CGSC:7695
phoB60						CGSC:7699
phoB63						CGSC:7701
phoB55						CGSC:7703
phoB763(del)::kan					PMID:16738554	CGSC:102352
phoB in strain KT202			Resistant to	Resistant to Tellurite	PMID:2944476	Strain: KT202	table 1
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0389	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCGAGACGTATTCTGGTCGT Primer 2:CCAAAGCGGGTTGAAAAACGATA
6A12	Kohara Phage	Genobase	PMID:3038334
9G9	Kohara Phage	Genobase	PMID:3038334
zai-3053::Tn10	Linked marker	CAG18091 = CGSC7336^[5]	est. P1 cotransduction: 44% ^[6] Synonyms:zaj-3053::Tn10 nnnCAG18091 also carries proC9999 (CGSC).
tsx-247::Tn10	Linked marker	CAG12148 = CGSC7337^[5]	est. P1 cotransduction: 59% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10728	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10728	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000719	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945046	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0721	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001389	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PhoB
Synonyms	B0399^[2], PhoT^[2], positive response regulator for pho regulon (and asr gene), sensor is PhoR (or CreC)^[2], phosphate regulon transcriptional regulatory protein PhoB^[2], , ECK0393, JW0389, phoRc, b0399
Product description	PhoB transcriptional dual regulator^[2]^[3] Positive response regulator for pho regulon, two-component system^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000156	two-component response regulator activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001789	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000156	two-component response regulator activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001867	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000156	two-component response regulator activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011879	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000160	two-component signal transduction system (phosphorelay)	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001789	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000160	two-component signal transduction system (phosphorelay)	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001867	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000160	two-component signal transduction system (phosphorelay)	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011879	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000160	two-component signal transduction system (phosphorelay)	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0902	P	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001867	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011879	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006350	transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0804	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001789	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001867	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006817	phosphate transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011879	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006817	phosphate transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0592	P	Seeded from EcoCyc (v14.0)	complete
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Interactions

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	nadE	PMID:16606699	Experiment(s):EBI-1136460
Protein	moaC	PMID:16606699	Experiment(s):EBI-1136460
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MARRILVVED EAPIREMVCF VLEQNGFQPV EAEDYDSAVN QLNEPWPDLI LLDWMLPGGS GIQFIKHLKR ESMTRDIPVV MLTARGEEED RVRGLETGAD DYITKPFSPK ELVARIKAVM RRISPMAVEE VIEMQGLSLD PTSHRVMAGE EPLEMGPTEF KLLHFFMTHP ERVYSREQLL NHVWGTNVYV EDRTVDVHIR RLRKALEPGG HDRMVQTVRG TGYRFSTRF
Length	229
Mol. Wt	26.432 kDa
pI	5.5 (calculated)
Extinction coefficient	23,950 - 24,075 (calc based on 5 Y, 3 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	149..225	PF00486 Transcriptional regulatory protein, C terminal	PMID:19920124
Domain	5..117	PF00072 Response regulator receiver domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=phoB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128384	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945046	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001389	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AFJ5	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10728	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10728	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945046	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000719	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0721	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	212	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	212	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	163	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
phoBR
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:416346..416386 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0399 (EcoliWiki Page)	NCBI GEO profiles for phoB
microarray	GenExpDB:b0399 (EcoliWiki Page)	Summary of data for phoB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (416077..416435) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ20; Well:H9^[7]
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Notes

Accessions Related to phoB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10728	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0721	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0399
EcoGene	EcoGene:EG10728	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000719	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001389	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT5G61380 (score: 1.000; bootstrap: 100%) AT5G60100 (score: 0.083)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0183884 (score: 1.000; bootstrap: 71%) DDB0191388 (score: 1.000; bootstrap: 62%) DDB0220512 (score: 0.106) DDB0231984 (score: 0.080) DDB0185194 (score: 0.077) DDB0187191 (score: 0.076) DDB0191306 (score: 0.075) DDB0204819 (score: 0.075) DDB0218392 (score: 0.066) DDB0218263 (score: 0.065) DDB0187593 (score: 0.065) DDB0185325 (score: 0.061) DDB0217795 (score: 0.060) DDB0220485 (score: 0.058) DDB0191407 (score: 0.058) DDB0188118 (score: 0.058) DDB0216671 (score: 0.056) DDB0206390 (score: 0.052)	From Inparanoid:20070104
Oryza gramene	Q6H805 (score: 1.000; bootstrap: 63%) Q94LW1 (score: 0.703) Q5SML5 (score: 0.284) Q6K8X6 (score: 0.277) Q67W50 (score: 0.231) Q948L2 (score: 0.203) Q7Y0W5 (score: 0.111) Q7Y0W3 (score: 0.111) Q7Y0W2 (score: 0.111) Q7Y0W4 (score: 0.108) Q6AU68 (score: 0.069) Q8H908 (score: 0.057)	From Inparanoid:20070104
Saccharomyces cerevisiae	YHR206W (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC8C94 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000033403 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	PHOB	From SHIGELLACYC
E. coli O157	PHOB	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00486 Transcriptional regulatory protein, C terminal
Superfamily (EcoliWiki Page)	SUPERFAMILY:52172
Pfam (EcoliWiki Page)	PF00072 Response regulator receiver domain
EcoCyc	EcoCyc:EG10728	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10728	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000719	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0721	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001389	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

phoB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to phoB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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