pheP:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

pheP

Gene Synonym(s)

ECK0568, b0576, JW0565[1], JW0565

Product Desc.

PheP phenylalanine APC transporter[2][3]

Phenylalanine-specific permease[4]

Product Synonyms(s)

phenylalanine transporter[1], B0576[2][1], PheP[2][1] , ECK0568, JW0565, b0576

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): pheP[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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The pheP GTG start was verified by start codon mutagenesis (Pi, 1991).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pheP

Mnemonic

Phenylalanine

Synonyms

ECK0568, b0576, JW0565[1], JW0565

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

12.96 minutes 

MG1655: 601182..602558
<gbrowseImage> name=NC_000913:601182..602558 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 582862..584238
<gbrowseImage> name=NC_012967:582862..584238 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 503942..505318
<gbrowseImage> name=NC_012759:503942..505318 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 601182..602558
<gbrowseImage> name=NC_007779:601182..602558 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 540514..541890
<gbrowseImage> name=NC_010473:540514..541890 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 653774..655150
<gbrowseImage> name=NC_010473:653774..655150 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔpheP (Keio:JW0565)

deletion

deletion

PMID:16738554

Shigen
CGSC8690[5]

ΔpheP785::kan

PMID:16738554

CGSC:102324


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0565

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAAAACGCGTCAACCGTATC

Primer 2:CCTTTCCGACGCAGCGTTTTAAA

21A9S

Kohara Phage

Genobase

PMID:3038334

purK79::Tn10

Linked marker

CAG12171 = CGSC7342[5]

est. P1 cotransduction: 10% [6]
Synonyms:purE79::Tn10

dsbG601::Tn10

Linked marker

CAG12149 = CGSC7346[5]

est. P1 cotransduction: 25% [6]
Synonyms:zbd-601::Tn10, zbe-601::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10708

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10708

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000700

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945199

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0702

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001976

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PheP

Synonyms

phenylalanine transporter[1], B0576[2][1], PheP[2][1] , ECK0568, JW0565, b0576

Product description

PheP phenylalanine APC transporter[2][3]

Phenylalanine-specific permease[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004841

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0006865

amino acid transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002293

P

Seeded from EcoCyc (v14.0)

complete

GO:0006865

amino acid transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004840

P

Seeded from EcoCyc (v14.0)

complete

GO:0006865

amino acid transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0029

P

Seeded from EcoCyc (v14.0)

complete

GO:0015171

amino acid transmembrane transporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002293

F

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002293

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004841

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004840

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0055085

transmembrane transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002293

P

Seeded from EcoCyc (v14.0)

complete

GO:0055085

transmembrane transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004840

P

Seeded from EcoCyc (v14.0)

complete

GO:0055085

transmembrane transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004841

P

Seeded from EcoCyc (v14.0)

complete

GO:0015823

phenylalanine transport

PMID:14702302

IGI: Inferred from Genetic Interaction

EcoliWiki:aroP EcoliWiki:livHMGF


P

Strain with the AroP and LIV-I/LS transport systems inactivated is able to transport phenylalanine.

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 12 predicted transmembrane domains

Daley et al. (2005) [7]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKNASTVSED TASNQEPTLH RGLHNRHIQL IALGGAIGTG LFLGIGPAIQ MAGPAVLLGY
GVAGIIAFLI MRQLGEMVVE EPVSGSFAHF AYKYWGPFAG FLSGWNYWVM FVLVGMAELT
AAGIYMQYWF PDVPTWIWAA AFFIIINAVN LVNVRLYGET EFWFALIKVL AIIGMIGFGL
WLLFSGHGGE KASIDNLWRY GGFFATGWNG LILSLAVIMF SFGGLELIGI TAAEARDPEK
SIPKAVNQVV YRILLFYIGS LVVLLALYPW VEVKSNSSPF VMIFHNLDSN VVASALNFVI
LVASLSVYNS GVYSNSRMLF GLSVQGNAPK FLTRVSRRGV PINSLMLSGA ITSLVVLINY
LLPQKAFGLL MALVVATLLL NWIMICLAHL RFRAAMRRQG RETQFKALLY PFGNYLCIAF
LGMILLLMCT MDDMRLSAIL LPVWIVFLFM AFKTLRRK
Length

458

Mol. Wt

50.679 kDa

pI

10.0 (calculated)

Extinction coefficient

95,340 - 95,715 (calc based on 16 Y, 13 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

27..458

PF00324 Amino acid permease

PMID:19920124

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<motif_map/>

tmhmm.php?gene=pheP&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pheP taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128559

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945199

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001976

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P24207

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10708

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10708

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945199

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000700

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0702

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

720

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

99

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

254

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

pheP

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:601162..601202 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0576 (EcoliWiki Page)

NCBI GEO profiles for pheP

microarray

GenExpDB:b0576 (EcoliWiki Page)

Summary of data for pheP from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (600996..601184) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ16; Well:E6[8]

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Notes

Accessions Related to pheP Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10708

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0702

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0576

EcoGene

EcoGene:EG10708

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000700

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001976

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000013870 (score: 1.000; bootstrap: 100%)
  • ENSANGP00000016244 (score: 0.231)
  • YP_026258 (score: 0.364)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000020051 (score: 1.000; bootstrap: 100%)
  • ENSBTAP00000019910 (score: 1.000; bootstrap: 100%)
  • ENSBTAP00000021266 (score: 0.708)
  • ENSBTAP00000009736 (score: 0.672)
  • ENSBTAP00000023826 (score: 0.660)
  • ENSBTAP00000020054 (score: 0.651)
  • ENSBTAP00000020048 (score: 0.629)
  • ENSBTAP00000020050 (score: 0.613)
  • ENSBTAP00000004741 (score: 0.610)
  • ENSBTAP00000012324 (score: 0.593)
  • ENSBTAP00000015329 (score: 0.578)
  • ENSBTAP00000017752 (score: 0.568)
  • ENSBTAP00000019058 (score: 0.481)
  • ENSBTAP00000013304 (score: 0.471)
  • ENSBTAP00000019911 (score: 0.432)
  • ENSBTAP00000010274 (score: 0.417)
  • ENSBTAP00000004740 (score: 0.396)
  • ENSBTAP00000012086 (score: 0.363)
  • ENSBTAP00000031960 (score: 0.327)
  • ENSBTAP00000004738 (score: 0.324)
  • ENSBTAP00000019053 (score: 0.240)
  • ENSBTAP00000004742 (score: 0.216)
  • ENSBTAP00000033212 (score: 0.199)
  • ENSBTAP00000013958 (score: 0.112)
  • ENSBTAP00000007774 (score: 0.101)
  • YP_026258 (score: 0.320)

From Inparanoid:20070104

Caenorhabditis briggsae

  • YP_026258 (score: 0.354)
  • WBGene00029162 (score: 1.000; bootstrap: 89%)
  • WBGene00029182 (score: 1.000; bootstrap: 72%)
  • WBGene00038808 (score: 0.573)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000022120 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000009958 (score: 0.316)
  • ENSCAFP00000024875 (score: 0.315)
  • ENSCAFP00000010331 (score: 0.302)
  • ENSCAFP00000004025 (score: 0.286)
  • ENSCAFP00000021989 (score: 0.260)
  • ENSCAFP00000009940 (score: 0.239)
  • ENSCAFP00000018083 (score: 0.233)
  • ENSCAFP00000023985 (score: 0.175)
  • YP_026258 (score: 0.216)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000015092 (score: 1.000; bootstrap: 100%)
  • ENSCINP00000009114 (score: 0.481)
  • ENSCINP00000015061 (score: 0.376)
  • YP_026258 (score: 0.307)

From Inparanoid:20070104

Drosophila melanogaster

  • YP_026258 (score: 0.367)
  • FBgn0031064 (score: 1.000; bootstrap: 100%)
  • FBgn0037203 (score: 0.228)
  • FBgn0036764 (score: 0.225)
  • FBgn0036984 (score: 0.197)
  • FBgn0036493 (score: 0.193)

From Inparanoid:20070104

Drosophila pseudoobscura

  • YP_026258 (score: 0.366)
  • GA11681-PA (score: 1.000; bootstrap: 100%)
  • GA18954-PA (score: 0.224)
  • GA10777-PA (score: 0.221)
  • GA12151-PA (score: 0.196)
  • GA20214-PA (score: 0.157)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000015210 (score: 1.000; bootstrap: 100%)
  • ENSGALP00000006942 (score: 1.000; bootstrap: 100%)
  • ENSGALP00000022132 (score: 0.447)
  • ENSGALP00000027546 (score: 0.405)
  • ENSGALP00000009182 (score: 0.352)
  • YP_026258 (score: 0.316)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000231706 (score: 1.000; bootstrap: 100%)
  • ENSP00000298085 (score: 0.285)
  • ENSP00000004531 (score: 0.249)
  • ENSP00000340324 (score: 0.218)
  • ENSP00000215750 (score: 0.200)
  • YP_026258 (score: 0.220)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000018385 (score: 1.000; bootstrap: 78%)
  • YP_026258 (score: 0.220)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000017922 (score: 1.000; bootstrap: 95%)
  • ENSMODP00000023784 (score: 0.295)
  • ENSMODP00000006229 (score: 0.289)
  • ENSMODP00000011481 (score: 0.267)
  • ENSMODP00000020049 (score: 0.217)
  • YP_026258 (score: 0.204)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000026872 (score: 1.000; bootstrap: 100%)
  • ENSPTRP00000034277 (score: 0.262)
  • ENSPTRP00000009787 (score: 0.219)
  • ENSPTRP00000024285 (score: 0.199)
  • ENSPTRP00000037822 (score: 0.092)
  • YP_026258 (score: 0.220)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000002560 (score: 1.000; bootstrap: 100%)
  • ENSRNOP00000001234 (score: 0.318)
  • ENSRNOP00000014809 (score: 0.301)
  • ENSRNOP00000005580 (score: 0.298)
  • ENSRNOP00000038036 (score: 0.296)
  • ENSRNOP00000035746 (score: 0.085)
  • YP_026258 (score: 0.353)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000137252 (score: 1.000; bootstrap: 86%)
  • NEWSINFRUP00000169769 (score: 0.552)
  • NEWSINFRUP00000147106 (score: 0.495)
  • NEWSINFRUP00000168050 (score: 0.290)
  • YP_026258 (score: 0.360)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00012714001 (score: 1.000; bootstrap: 100%)
  • GSTENP00006821001 (score: 0.266)
  • GSTENP00003539001 (score: 0.263)
  • GSTENP00033801001 (score: 0.244)
  • GSTENP00035216001 (score: 0.241)
  • GSTENP00018927001 (score: 0.238)
  • GSTENP00008630001 (score: 0.236)
  • GSTENP00019046001 (score: 0.207)
  • GSTENP00007050001 (score: 0.173)
  • GSTENP00017133001 (score: 0.077)
  • YP_026258 (score: 0.231)

From Inparanoid:20070104

Shigella flexneri

PHEP

From SHIGELLACYC

E. coli O157

PHEP

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00324 Amino acid permease

EcoCyc

EcoCyc:EG10708

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10708

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000700

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0702

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001976

Escherichia coli str. K-12 substr. MG1655

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  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  8. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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