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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	pheA
Gene Synonym(s)	ECK2596, b2599, JW2580^[1], JW2580
Product Desc.	PheA^[2]^[3]; Component of CHORISMUTPREPHENDEHYDRAT-CPLX^[2] Phenylalanine synthesis, bifunctional: chorismate mutase (N) and prephenate dehydratase (central); also contains Phe-binding regulatory domain (C); FPA resistance^[4]
Product Synonyms(s)	fused chorismate mutase P^[1], prephenate dehydratase^[1], B2599^[2]^[1], PheA^[2]^[1] , ECK2596, JW2580, b2599
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pheLA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	pheA
Mnemonic	Phenylalanine
Synonyms	ECK2596, b2599, JW2580^[1], JW2580
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	58.96 minutes	MG1655: 2735767..2736927 <gbrowseImage> name=NC_000913:2735767..2736927 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2659152..2660312 <gbrowseImage> name=NC_012967:2659152..2660312 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2621579..2622739 <gbrowseImage> name=NC_012759:2621579..2622739 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2736401..2737561 <gbrowseImage> name=NC_007779:2736401..2737561 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2827532..2828692 <gbrowseImage> name=NC_010473:2827532..2828692 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
pheA(del) (Keio:JW2580)	deletion	deletion			PMID:16738554	Shigen CGSC10048^[5]
pheA18::Tn10	Insertion at 2,736,078 bp in MG1655 (NC_000913)				adapted from Nichols et al.^[6]	CAG12158 = CGSC7421^[5]	Synonyms:
pheA3::Mu						CGSC:4849
pheA18::Tn10						CGSC:7100
pheA97						CGSC:7678
pheA7						CGSC:9947
pheA905						CGSC:10750
pheA2::Mu						CGSC:11369
pheA3141::Tn10kan					PMID:2540407	CGSC:40092
pheA762(del)::kan					PMID:16738554	CGSC:102318
pheA			Resistant to	Fluoro(4)phenylalanine resistance	PMID:4934063		table 2
pheA30			Resistant to	Resistant to Fluoro(4)phenylalanine	PMID:1514806
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2580	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACATCGGAAAACCCGTTACT Primer 2:CCGGTTGGATCAACAGGCACTAC
21D7	Kohara Phage	Genobase	PMID:3038334
E8F2	Kohara Phage	Genobase	PMID:3038334
nadB51::Tn10	Linked marker	CAG18480 = CGSC7419^[5]	est. P1 cotransduction: 37% ^[6]
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[5]	est. P1 cotransduction: % ^[6] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10707	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10707	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000699	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947081	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0701	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008545	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PheA
Synonyms	fused chorismate mutase P^[1], prephenate dehydratase^[1], B2599^[2]^[1], PheA^[2]^[1] , ECK2596, JW2580, b2599
Product description	PheA^[2]^[3]; Component of CHORISMUTPREPHENDEHYDRAT-CPLX^[2] Phenylalanine synthesis, bifunctional: chorismate mutase (N) and prephenate dehydratase (central); also contains Phe-binding regulatory domain (C); FPA resistance^[4]
EC number (for enzymes)	5.4.99.5^[1] 4.2.1.51^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004106	chorismate mutase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008242	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004106	chorismate mutase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010952	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004106	chorismate mutase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:5.4.99.5	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004106	chorismate mutase activity	PMID:7030214	IMP: Inferred from Mutant Phenotype		F	Seeded from EcoCyc (v14.0)	complete
	GO:0004106	chorismate mutase activity	PMID:9497350	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0004664	prephenate dehydratase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001086	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004664	prephenate dehydratase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008242	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004664	prephenate dehydratase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018528	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004664	prephenate dehydratase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.2.1.51	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004664	prephenate dehydratase activity	PMID:7030214	IMP: Inferred from Mutant Phenotype		F	Seeded from EcoCyc (v14.0)	complete
	GO:0004664	prephenate dehydratase activity	PMID:9497350	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008242	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010952	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006571	tyrosine biosynthetic process	PMID:7030214	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc (v14.0)	complete
	GO:0009094	L-phenylalanine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001086	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009094	L-phenylalanine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008242	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009094	L-phenylalanine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010952	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009094	L-phenylalanine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018528	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009094	L-phenylalanine biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0584	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009094	L-phenylalanine biosynthetic process	PMID:7030214	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc (v14.0)	complete
	GO:0042803	protein homodimerization activity	PMID:4557843	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of CHORISMUTPREPHENDEHYDRAT-CPLX	could be indirect
Protein	eutD		PMID:16606699	Experiment(s):EBI-1143449
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTSENPLLAL REKISALDEK LLALLAERRE LAVEVGKAKL LSHRPVRDID RERDLLERLI TLGKAHHLDA HYITRLFQLI IEDSVLTQQA LLQQHLNKIN PHSARIAFLG PKGSYSHLAA RQYAARHFEQ FIESGCAKFA DIFNQVETGQ ADYAVVPIEN TSSGAINDVY DLLQHTSLSI VGEMTLTIDH CLLVSGTTDL STINTVYSHP QPFQQCSKFL NRYPHWKIEY TESTSAAMEK VAQAKSPHVA ALGSEAGGTL YGLQVLERIE ANQRQNFTRF VVLARKAINV SDQVPAKTTL LMATGQQAGA LVEALLVLRN HNLIMTRLES RPIHGNPWEE MFYLDIQANL ESAEMQKALK ELGEITRSMK VLGCYPSENV VPVDPT
Length	386
Mol. Wt	43.111 kDa
pI	6.7 (calculated)
Extinction coefficient	27,390 - 27,890 (calc based on 11 Y, 2 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	11..91	PF01817 Chorismate mutase type II	PMID:19920124
Domain	106..288	PF00800 Prephenate dehydratase	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pheA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130520	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947081	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008545	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A9J8	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10707	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10707	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947081	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000699	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0701	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	1783	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	862	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1224	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pheLA
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2735747..2735787 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2599 (EcoliWiki Page)	NCBI GEO profiles for pheA
microarray	GenExpDB:b2599 (EcoliWiki Page)	Summary of data for pheA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to pheA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10707	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0701	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2599
EcoGene	EcoGene:EG10707	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000699	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008545	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT3G07630 (score: 1.000; bootstrap: 100%) AT1G11790 (score: 0.416) AT1G08250 (score: 0.410) AT2G27820 (score: 0.401) AT5G22630 (score: 0.358) AT3G44720 (score: 0.349)	From Inparanoid:20070104
Oryza gramene	Q6Z3Y3 (score: 1.000; bootstrap: 100%) Q650W1 (score: 0.440) Q650V6 (score: 0.438) Q7XLY2 (score: 0.426) Q8H0A1 (score: 0.152)	From Inparanoid:20070104
Saccharomyces cerevisiae	YNL316C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC30D106 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000018766 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	PHEA	From SHIGELLACYC
E. coli O157	PHEA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01817 Chorismate mutase type II
Superfamily (EcoliWiki Page)	SUPERFAMILY:48600
Panther (EcoliWiki Page)	PTHR21022:SF0
Superfamily (EcoliWiki Page)	SUPERFAMILY:55021
Pfam (EcoliWiki Page)	PF00800 Prephenate dehydratase
EcoCyc	EcoCyc:EG10707	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10707	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000699	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0701	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008545	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 ^6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

pheA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to pheA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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