{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	pcm
Gene Synonym(s)	ECK2738, b2743, JW2713^[1], JW2713
Product Desc.	L-isoaspartate protein carboxylmethyltransferase type II^[2]^[3] Protein-L-isoaspartate O-methyltransferase, SAM-dependent; repair of isoaspartyl residues; mutant is suseptible to secondary stresses and has a competitive disadvantage in stationary phase; monomeric; overexpression confers tolerance to thermal str^[4]
Product Synonyms(s)	L-isoaspartate protein carboxylmethyltransferase type II^[1], B2743^[2]^[1], Pcm^[2]^[1] , ECK2738, JW2713, b2743
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): surE-pcm^[2]
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

Pcm may work with SurE: they are co-transcribed and have addictive effects on isoaspartyl accumulation during stationary phase. Pcm was originally thought to be required for stationary phase and heat-stress survival, but this was due to a secondary rpoS mutation. otherPDB (P. furiosus): 1JG1.^[4]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	pcm
Mnemonic	Protein carboxyl methyltransferase
Synonyms	ECK2738, b2743, JW2713^[1], JW2713
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	61.79 minutes	MG1655: 2867541..2866915 <gbrowseImage> name=NC_000913:2866915..2867541 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2764380..2763754 <gbrowseImage> name=NC_012967:2763754..2764380 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2752727..2753353 <gbrowseImage> name=NC_012759:2752727..2753353 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2868175..2867549 <gbrowseImage> name=NC_007779:2867549..2868175 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2960083..2959457 <gbrowseImage> name=NC_010473:2959457..2960083 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2866918	Edman degradation	PMID:1860862
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
Δpcm (Keio:JW2713)	deletion	deletion	PMID:16738554	Shigen CGSC10135^[5]
Δpcm-748::kan			PMID:16738554	CGSC:102262
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2713	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGTAAGCAGACGCGTACAAGC Primer 2:CCAGCCAGCTCACCCTTCACTAA
25D2	Kohara Phage	Genobase	PMID:3038334
1B5	Kohara Phage	Genobase	PMID:3038334
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[5]	est. P1 cotransduction: 17% ^[6] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
cysI95::Tn10	Linked marker	CAG12173 = CGSC7425^[5]	est. P1 cotransduction: 50% ^[6] Synonyms:cysC95::Tn10
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10689	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10689	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000682	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944923	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0683	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009006	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Pcm
Synonyms	L-isoaspartate protein carboxylmethyltransferase type II^[1], B2743^[2]^[1], Pcm^[2]^[1] , ECK2738, JW2713, b2743
Product description	L-isoaspartate protein carboxylmethyltransferase type II^[2]^[3] Protein-L-isoaspartate O-methyltransferase, SAM-dependent; repair of isoaspartyl residues; mutant is suseptible to secondary stresses and has a competitive disadvantage in stationary phase; monomeric; overexpression confers tolerance to thermal str^[4]
EC number (for enzymes)	2.1.1.77^[1]
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004719	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00090	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004719	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000682	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004719	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.1.1.77	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00090	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006464	protein modification process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00090	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006464	protein modification process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000682	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008168	methyltransferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0489	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030091	protein repair	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00090	P	Seeded from EcoCyc (v14.0)	complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MVSRRVQALL DQLRAQGIQD EQVLNALAAV PREKFVDEAF EQKAWDNIAL PIGQGQTISQ PYMVARMTEL LELTPQSRVL EIGTGSGYQT AILAHLVQHV CSVERIKGLQ WQARRRLKNL DLHNVSTRHG DGWQGWQARA PFDAIIVTAA PPEIPTALMT QLDEGGILVL PVGEEHQYLK RVRRRGGEFI IDTVEAVRFV PLVKGELA
Length	208
Mol. Wt	23.257 kDa
pI	7.0 (calculated)
Extinction coefficient	26,470 - 26,595 (calc based on 3 Y, 4 W, and 1 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0A7A5
Domain	4..207	PF01135 Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)		PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pcm taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130650	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944923	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009006	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A7A5	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10689	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10689	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944923	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000682	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0683	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	556	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	167	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	259	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
surE-pcm
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:2867521..2867561 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2743 (EcoliWiki Page)	NCBI GEO profiles for pcm
microarray	GenExpDB:b2743 (EcoliWiki Page)	Summary of data for pcm from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to pcm Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10689	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0683	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2743
EcoGene	EcoGene:EG10689	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000682	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009006	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000020776 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000008738 (score: 1.000; bootstrap: 100%) ENSAPMP00000002493 (score: 0.343)	From Inparanoid:20070104
Arabidopsis thaliana	AT3G48330 (score: 1.000; bootstrap: 100%) AT5G50240 (score: 0.292)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000013464 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00030890 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00003954 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000000571 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000014932 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-040426-1738 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040425-2678 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040801-279 (score: 0.660) ZDB-GENE-990415-134 (score: 0.660)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0214947 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0015276 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA15271-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000007384 (score: 1.000; bootstrap: 100%) ENSGALP00000020199 (score: 0.565)	From Inparanoid:20070104
Homo sapiens	ENSP00000239377 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000020973 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:97502 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q6ZJ17 (score: 1.000; bootstrap: 100%) Q7XUQ2 (score: 0.671) Q43209 (score: 0.671)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000031911 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000019623 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC8698 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000151732 (score: 1.000; bootstrap: 100%) NEWSINFRUP00000134699 (score: 0.528)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00032200001 (score: 1.000; bootstrap: 100%) GSTENP00035418001 (score: 0.639)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000026905 (score: 1.000; bootstrap: 87%)	From Inparanoid:20070104
Shigella flexneri	PCM	From SHIGELLACYC
E. coli O157	PCM	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR11579:SF0
Superfamily (EcoliWiki Page)	SUPERFAMILY:53335
Pfam (EcoliWiki Page)	PF01135 Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
EcoCyc	EcoCyc:EG10689	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10689	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000682	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0683	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009006	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

pcm:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to pcm Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search