{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	pbpC
Gene Synonym(s)	ECK2515, b2519, JW2503, yfgN^[1], yfgN
Product Desc.	putative peptidoglycan enzyme^[2]^[3] Penicillin-binding protein PBP1C murein transglycosylase^[4]
Product Synonyms(s)	fused transglycosylase^[1], transpeptidase^[1], B2519^[2]^[1], YfgN^[2]^[1], PbpC^[2]^[1], PBP1C^[2]^[1] , ECK2515, JW2503, yfgN, b2519
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pbpC^[2]
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

PbpC has a penicillin-insensitive transglycosylase N-terminal domain. PbpC has a transpeptidase-like C-terminal domain that does not seem to be functional. PbpC is a membrane-bound periplasmic enzyme that has an uncleaved signal anchor^[4]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	pbpC
Mnemonic	Penicillin-binding protein
Synonyms	ECK2515, b2519, JW2503, yfgN^[1], yfgN
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	56.97 minutes	MG1655: 2645347..2643035 <gbrowseImage> name=NC_000913:2643035..2645347 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2568144..2565832 <gbrowseImage> name=NC_012967:2565832..2568144 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2528840..2531152 <gbrowseImage> name=NC_012759:2528840..2531152 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2645981..2643669 <gbrowseImage> name=NC_007779:2643669..2645981 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2737112..2734800 <gbrowseImage> name=NC_010473:2734800..2737112 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔpbpC (Keio:JW2503)	deletion	deletion			PMID:16738554	Shigen
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2503	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCCTCGCTTGTTAACCAAACG Primer 2:CCTTGCATGACAAATTTCACTGT
2D5	Kohara Phage	Genobase	PMID:3038334
7F8	Kohara Phage	Genobase	PMID:3038334
purC80::Tn10	Linked marker	CAG18470 = CGSC7413^[5]	est. P1 cotransduction: 10% ^[6]
zfh-208::Tn10	Linked marker	CAG18481 = CGSC7417^[5]	est. P1 cotransduction: 48% ^[6] Synonyms:zff-208::Tn10
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7322	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14210	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003894	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947152	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3962	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008294	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PbpC
Synonyms	fused transglycosylase^[1], transpeptidase^[1], B2519^[2]^[1], YfgN^[2]^[1], PbpC^[2]^[1], PBP1C^[2]^[1] , ECK2515, JW2503, yfgN, b2519
Product description	putative peptidoglycan enzyme^[2]^[3] Penicillin-binding protein PBP1C murein transglycosylase^[4]
EC number (for enzymes)	2.4.1.-^[1] 3.4.-^[1]
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0030288	outer membrane-bounded periplasmic space				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008360	regulation of cell shape	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0133	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008658	penicillin binding	PMID:1103132	IDA: Inferred from Direct Assay		F	Table 3 shows ampicillin inhibiting cell division when binding to PBP 3.	complete
	GO:0009252	peptidoglycan biosynthetic process	PMID:7030331	IDA: Inferred from Direct Assay		P	Figure 2 shows formation of peptidoglycan when placed in a solution with penicillin binding protein 3.	complete
	GO:0009252	peptidoglycan biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0573	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009274	peptidoglycan-based cell wall	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001264	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9906	C	Seeded from EcoCyc (v14.0)	complete
	GO:0051781	positive regulation of cell division	PMID:1103132	IDA: Inferred from Direct Assay		P	Table 3 shows inhibition of cell division when penicillin binding protein 3 is competing with ampicillin.	complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	lon	PMID:16606699	Experiment(s):EBI-1143101
Protein	htpG	PMID:16606699	Experiment(s):EBI-1143101
Protein	rplB	PMID:16606699	Experiment(s):EBI-1143101
Protein	maeB	PMID:16606699	Experiment(s):EBI-1143101
Protein	groL	PMID:16606699	Experiment(s):EBI-1143101
Protein	rpsE	PMID:16606699	Experiment(s):EBI-1143101
Protein	clpB	PMID:16606699	Experiment(s):EBI-1143101
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
Inner membrane	PMID:10542235	EchoLocation:pbpC
membrane
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MPRLLTKRGC WITLAAAPFL LFLAAWGADK LWPLPLHEVN PARVVVAQDG TPLWRFADAD GIWRYPVTIE DVSPRYLEAL INYEDRWFWK HPGVNPFSVA RAAWQDLTSG RVISGGSTLT MQVARLLDPH PKTFGGKIRQ LWRALQLEWH LSKREILTLY LNRAPFGGTL QGIGAASWAY LGKSPANLSY SEAAMLAVLP QAPSRLRPDR WPERAEAARN KVLERMAVQG VWSREQVKES REEPIWLAPR QMPQLAPLFS RMMLGKSKSD KITTTLDAGL QRRLEELAQN WKGRLPPRSS LAMIVVDHTD MRVRGWVGSV DLNDDSRFGH VDMVNSIRSP GSVLKPFVYG LALDEGLIHP ASLLQDVPRR TGDYRPGNFD SGFHGPISMS EALVRSLNLP AVQVLEAYGP KRFAAKLRNV GLPLYLPNGA APNLSLILGG AGAKLEDMAA AYTAFARHGK AGKLRLQPDD PLLERPLMSS GAAWIIRRIM ADEAQPLPDS ALPRVAPLAW KTGTSYGYRD AWAIGVNARY VIGIWTGRPD GTPVVGQFGF ASAVPLLNQV NNILLSRSAN LPEDPRPNSV TRGVICWPGG QSLPEGDGNC RRRLATWLLD GSQPPTLLLP EQEGINGIRF PIWLDENGKR VAADCPQARQ EMINVWPLPL EPWLPASERR AVRLPPASTS CPPYGHDAQL PLQLTGVRDG AIIKRLPGAA EATLPLQSSG GAGERWWFLN GEPLTERGRN VTLHLTDKGD YQLLVMDDVG QIATVKFVMQ
Length	770
Mol. Wt	85.068 kDa
pI	9.9 (calculated)
Extinction coefficient	172,340 - 172,965 (calc based on 16 Y, 27 W, and 5 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	301..548	PF00905 Penicillin binding protein transpeptidase domain	PMID:19920124
Domain	49..227	PF00912 Transglycosylase	PMID:19920124
Domain	681..767	PF06832 Penicillin-Binding Protein C-terminus Family	PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pbpC taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130444	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947152	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008294	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P76577	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7322	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14210	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947152	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003894	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3962	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	18	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	11	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	2^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pbpC
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:2645327..2645367 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2519 (EcoliWiki Page)	NCBI GEO profiles for pbpC
microarray	GenExpDB:b2519 (EcoliWiki Page)	Summary of data for pbpC from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to pbpC Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7322	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3962	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2519
EcoGene	EcoGene:EG14210	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003894	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008294	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000040947 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	PBPC	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00905 Penicillin binding protein transpeptidase domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:56601
Pfam (EcoliWiki Page)	PF00912 Transglycosylase
Pfam (EcoliWiki Page)	PF06832 Penicillin-Binding Protein C-terminus Family
EcoCyc	EcoCyc:G7322	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14210	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003894	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3962	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008294	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

pbpC:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to pbpC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search