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Gene |

Product(s) |

Expression|

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Standard Name	pabA
Gene Synonym(s)	ECK3348, b3360, JW3323^[1], JW3323
Product Desc.	PabA^[2]^[3]; Component of aminodeoxychorismate synthase^[2]^[3]; para-aminobenzoate synthase multi-enzyme complex^[2]^[3] Sulfonamide resistance; p-aminobenzoate biosynthesis^[4]
Product Synonyms(s)	aminodeoxychorismate synthase, subunit II^[1], B3360^[2]^[1], PabA^[2]^[1], para-aminobenzoate synthase glutamine amidotransferase component II^[2]^[1] , ECK3348, JW3323, b3360
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pabA^[2], yhfG-fic-pabA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	pabA
Mnemonic	Para-Aminobenzoate
Synonyms	ECK3348, b3360, JW3323^[1], JW3323
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	75.18 minutes	MG1655: 3488851..3488288 <gbrowseImage> name=NC_000913:3488288..3488851 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3418734..3418171 <gbrowseImage> name=NC_012967:3418171..3418734 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3375445..3376008 <gbrowseImage> name=NC_012759:3375445..3376008 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4149587..4150150 <gbrowseImage> name=NC_007779:4149587..4150150 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3586596..3586033 <gbrowseImage> name=NC_010473:3586033..3586596 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
pabA(del) (Keio:JW3323)	deletion	deletion			PMID:16738554	Shigen CGSC10483^[5]
pabAC79S		C79S		10000-fold decrease in catalytic efficiency			seeded from UniProt:P00903
pabAH168Q		H168Q		Loss of activity			seeded from UniProt:P00903
pabAE170A		E170A		150-fold decrease in catalytic efficiency			seeded from UniProt:P00903
pabAE170D		E170D		4-fold decrease in catalytic efficiency			seeded from UniProt:P00903
pabAE170K,Q		E170K,Q		Loss of activity			seeded from UniProt:P00903
pabA1						CGSC:11334
pabA2						CGSC:13790
pabA768::kan					PMID:16738554	CGSC:102240
pabA in strain IB3			Resistant to	Resistant to Sulfonamide	PMID:6392473	Strain: IB3	Table 2
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3323	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATCCTGCTTATAGATAACTA Primer 2:CCGCGATGCAGGAAATTAGCCAG
15C3	Kohara Phage	Genobase	PMID:3038334
4C5	Kohara Phage	Genobase	PMID:3038334
smg-3082::Tn10	Linked marker	CAG12071 = CGSC7444^[5]	est. P1 cotransduction: 5% ^[6] Synonyms:zhb-3082::Tn10, zhd-3082::Tn10
zhf-3084::Tn10	Linked marker	CAG18456 = CGSC7576^[5]	est. P1 cotransduction: 60% ^[6] Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10682	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10682	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000675	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947873	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0676	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010983	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PabA
Synonyms	aminodeoxychorismate synthase, subunit II^[1], B3360^[2]^[1], PabA^[2]^[1], para-aminobenzoate synthase glutamine amidotransferase component II^[2]^[1] , ECK3348, JW3323, b3360
Product description	PabA^[2]^[3]; Component of aminodeoxychorismate synthase^[2]^[3]; para-aminobenzoate synthase multi-enzyme complex^[2]^[3] Sulfonamide resistance; p-aminobenzoate biosynthesis^[4]
EC number (for enzymes)	6.3.5.8^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000991	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001317	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006220	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011702	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004049	anthranilate synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006221	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006541	glutamine metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001317	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006541	glutamine metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011702	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006541	glutamine metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0315	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006221	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009058	biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006220	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046656	folic acid biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0289	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046820	4-amino-4-deoxychorismate synthase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.6.1.85	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of aminodeoxychorismate synthase	could be indirect
Protein	clpX		PMID:16606699	Experiment(s):EBI-1145690
Protein	groL		PMID:16606699	Experiment(s):EBI-1145690
Protein	hisA		PMID:16606699	Experiment(s):EBI-1145690
Protein	glpC		PMID:16606699	Experiment(s):EBI-1145690
Protein	Subunits of para-aminobenzoate synthase multi-enzyme complex	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MILLIDNYDS FTWNLYQYFC ELGADVLVKR NDALTLADID ALKPQKIVIS PGPCTPDEAG ISLDVIRHYA GRLPILGVCL GHQAMAQAFG GKVVRAAKVM HGKTSPITHN GEGVFRGLAN PLTVTRYHSL VVEPDSLPAC FDVTAWSETR EIMGIRHRQW DLEGVQFHPE SILSEQGHQL LANFLHR
Length	187
Mol. Wt	20.771 kDa
pI	6.6 (calculated)
Extinction coefficient	23,950 - 24,450 (calc based on 5 Y, 3 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	3..187	PF00117 Glutamine amidotransferase class-I		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pabA taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131239	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947873	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010983	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P00903	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10682	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10682	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947873	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000675	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0676	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	380	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	80	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	194	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pabA
yhfG-fic-pabA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3488831..3488871 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3360 (EcoliWiki Page)	NCBI GEO profiles for pabA
microarray	GenExpDB:b3360 (EcoliWiki Page)	Summary of data for pabA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to pabA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10682	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0676	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3360
EcoGene	EcoGene:EG10682	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000675	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010983	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT5G57890 (score: 1.000; bootstrap: 98%) AT1G25220 (score: 0.899) AT1G24909 (score: 0.662) AT1G25083 (score: 0.662) AT1G25155 (score: 0.662) AT1G24807 (score: 0.638)	From Inparanoid:20070104
Saccharomyces cerevisiae	YKL211C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC15399c (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Shigella flexneri	PABA	From SHIGELLACYC
E. coli O157	PABA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00117 Glutamine amidotransferase class-I
Superfamily (EcoliWiki Page)	SUPERFAMILY:52317
EcoCyc	EcoCyc:EG10682	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10682	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000675	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0676	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010983	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

pabA:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

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Alleles and Phenotypes

Notes

Genetic Interactions

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Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to pabA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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